A5029536190- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Reproductive tract infections research
- Photodynamic Therapy Research Studies
- Nail Diseases and Treatments
- Synthesis and biological activity
- Medical and Biological Ozone Research
- Plant Pathogens and Fungal Diseases
- Synthesis and Biological Evaluation
- Immunodeficiency and Autoimmune Disorders
- Bee Products Chemical Analysis
- Redox biology and oxidative stress
- Nanoplatforms for cancer theranostics
- Breastfeeding Practices and Influences
- Parasitic Diseases Research and Treatment
- Hematopoietic Stem Cell Transplantation
- Mycobacterium research and diagnosis
- Click Chemistry and Applications
- Antimicrobial agents and applications
- Oral health in cancer treatment
- Trypanosoma species research and implications
- Mycotoxins in Agriculture and Food
- Biotechnology and Related Fields
- Advanced Drug Delivery Systems
- Oral microbiology and periodontitis research
Universidade Estadual de Maringá
2014-2025
Aim: To evaluate the efficacy of photodynamic inactivation (PDI) mediated by hypericin encapsulated in P-123 copolymeric micelles (P123-Hyp) alone and combination with fluconazole (FLU) against planktonic cells biofilm formation Candida species Materials & methods: PDI was performed using P123-Hyp an LED device irradiance 3.0 mW/cm2 . Results: Most isolates (70%) were completely inhibited concentrations up to 2.0 μmol/l HYP light fluence 16.2 J/cm2. FLU-resistant strains had synergic effect...
Candida infections have become a serious public health problem with high mortality rates, especially in immunocompromised patients, being albicans the major opportunistic pathogen responsible for systemic or invasive candidiasis. Commercially available antifungal agents are restricted and fungal resistance to such drugs has increased; therefore, development of more specific is necessary. Using assays activity, here we report that two new compounds 1,3,4-oxadiazoles class (LMM5 LMM11), which...
Vulvovaginal candidiasis (VVC) is a common vaginitis that affects women, especially in childbearing age, caused by Candida albicans almost 80% of cases. Considering the limited drug arsenal available and increasing fungal resistance profile, search for new therapeutic sources with low toxicity easy administration should be supported. Propolis has been used as traditional medicine multiple diseases, considering its particular composition pharmaceutical properties permits wide applicability;...
Paracoccidioidomycosis (PCM) is a neglected disease present in Latin America with difficulty treatment and occurrence of serious sequelae. Thus, the development alternative therapies imperative. In current work, two oxadiazole compounds (LMM5 LMM11) presented fungicidal activity against Paracoccidioides spp. The minimum inhibitory concentration values ranged from 1 to 32 μg/mL, synergic effect was observed for both when combined Amphotericin B. LMM5 LMM11 were able reduce CFU counts (≥2...
The present study aimed to characterize cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies. It was evaluated 12 clinical of C. samples: 4 asymptomatic (AS), a single episode vulvovaginal candidiasis (VVC) and recurrent (RVVC). We the ability adhere human cervical cancer cells (SiHa), yeast-SiHa interactions damage. All presented high adhesion capacity on SiHa cells. However, symptomatic (VVC RVVC) had higher filamentation after contact (24 h)...
Candida albicans is the most common species isolated from nosocomial bloodstream infections. Due to limited therapeutic arsenal and increase of drug resistance, there an urgent need for new antifungals. Therefore, antifungal activity against C. in vivo toxicity a 1,3,4-oxadiazole compound (LMM6) was evaluated. This selected by silico approach based on chemical similarity. LMM6 highly effective several clinical isolates, with minimum inhibitory concentration values ranging 8 32 µg/mL. also...
The aim of this study was to investigate possible associations between genetic polymorphisms IL17A G197A (rs2275913) and IL17F T7488C (rs763780) with Chagas Disease (CD) and/or the severity left ventricular systolic dysfunction (LVSD) in patients chronic cardiomyopathy (CCC). 260 150 controls conducted South Southeast regions Brazil. genotyping performed by PCR-RFLP. A allele A/A genotype were significantly increased their subgroups (patients CCC; CCC LVSD; severe LVSD) when compared control...
Invasive aspergillosis is one of the major causes morbidity and mortality among invasive fungal infections. The search for new antifungal drugs becomes imperative when existing are not able to efficiently treat these Ebselen, an organoselenium compound, already successfully approved in clinical trials as a repositioned drug treatment bipolar disorder prevention noise-induced hearing loss. In this study, we aimed reposition ebselen by showing effectiveness murine model. For this, BALB/c mice...
Candida krusei is one of the most common agents invasive candidiasis and candidemia worldwide, leading to high morbidity mortality rates. This species has become a problem due its intrinsic resistance reduced susceptibility azoles polyenes. Moreover, number antifungal drugs available for treatment limited, demonstrating urgent need discovery novel alternative therapies. In this work, in vivo vitro activities new oxadiazole (LMM11) were evaluated against C. krusei. The minimum inhibitory...
Candida albicans is the major pathogen isolated from nosocomial bloodstream infections, leading to higher mortality rates. Thus, due its clinical relevance, studies aiming understand host–pathogen interactions in C. infection are necessary. Therefore, we performed proteomic analysis using a murine model of serial systemic by evaluate possible changes protein profile over time. Firstly, observed reduction median survival time infected animals with increasing passage number, suggesting...
Aim: To study the behavior of Candida albicans in women with vulvovaginal candidiasis (VVC), recurrent VVC (RVVC) and asymptomatic (AS), regarding adhesion on HeLa cells their ability to express secreted aspartic proteinases (SAP) genes, agglutinin-like sequence (ALS) genes HWP1. Materials & methods: The was evaluated by colony-forming units, expressed were qRT-PCR. Results: AS isolates showed greater adhere when compared RVVC isolate. Nevertheless, isolate exhibited upregulation a large...
Aim: To compare the pathogenesis of vulvovaginal candidiasis by three Candida species in diabetic mice. Materials & methods: Estrogenized and mice were challenged with C. albicans, tropicalis glabrata. Results: Diabetic animals infected albicans maintained highest fungal burden, despite high levels proinflammatory cytokines (IL-6 TNF-α), respectively. For glabrata, results similar nondiabetic groups. Conclusion:C. was as invasive both more effective than This ability attributed to...
Aim: This study evaluated the antifungal efficacy of gentian violet (GV) in an experimental vulvovaginal candidiasis (VVC) model. Materials & methods: In vitro susceptibility and cytotoxicity assays were performed to validate potential safety GV. The was then vivo through comparative analysis fungal burden following treatment with GV or nystatin, as well assessment vaginal tissue by histology electron microscopy. Results: demonstrated a safe profile against C. albicans, significant decrease...