A5065701788- Hair Growth and Disorders
- Connexins and lens biology
- Skin and Cellular Biology Research
- Carcinogens and Genotoxicity Assessment
- Hormonal and reproductive studies
- Effects and risks of endocrine disrupting chemicals
- Corneal Surgery and Treatments
- Curcumin's Biomedical Applications
- Advanced Glycation End Products research
- Cytomegalovirus and herpesvirus research
- Skin Protection and Aging
- Biochemical and Molecular Research
- Wound Healing and Treatments
- Dermatology and Skin Diseases
- Sexual Differentiation and Disorders
- Nanoparticle-Based Drug Delivery
- Porphyrin Metabolism and Disorders
- Biochemical effects in animals
- Aldose Reductase and Taurine
- Protease and Inhibitor Mechanisms
- Herpesvirus Infections and Treatments
- Estrogen and related hormone effects
- Retinoids in leukemia and cellular processes
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
Northwest University
2015-2022
Northwest University
2017-2018
Radboud University Nijmegen
1980-2015
China XD Group (China)
2015
KU Leuven
2010-2013
Molecular Oncology (United States)
2008
Inserm
1987-1989
Centre de Biologie Structurale
1987-1988
Fondation Sophia Antipolis
1988
Universitat de les Illes Balears
1986
Dextran-coated SPIONs (DSPIONs) are synthesized and used as drug magnetic carriers to form conjugates of Dox–DSPIONs. The cytotoxicity, intracellular uptake behavior anti-tumor activity the investigated.
For the first time a method for culturing human hair follicle cells is described. The bovine eye lens capsule, basement membrane-like structure, used as substrate cultures. In culture medium supplemented with hydrocortisone and insulin about 70% of original follicles will form growing colonies diploid keratinocytes.
Magnetic nanoparticles (NPs) loaded with antitumor drugs in combination an external magnetic field (EMF)-guided delivery can improve the efficacy of treatment and may decrease serious side effects. The purpose this study was 1) to investigate application PEG modified GMNPs (PGMNPs) as a drug carrier chemotherapy compound doxorubicin (DOX) vitro; 2) evaluate therapeutic efficiency DOX-conjugated PGMNPs (DOX-PGMNPs) using EMF-guided vivo.First, DOX-PGMNPs were synthesized cytotoxicity assessed...
Oxidized low-density lipoprotein (oxLDL) accumulates early in atherosclerotic lesions and plays an important role the progressive formation of plaques. Endothelial derived microparticles (EMPs) form a heterogeneous population <1-μm particles that shed from endothelial membranes upon activation. While EMPs are shown to be involved pathophysiology progression, there is no report regarding relationship between oxLDL EMPs. In this study, we aim determine influence on microparticle release...
The isolated lenticular plasma membrane-cytoskeleton complex, when analysed by sodium dodecylsulphate-polyacrylamide gel electrophoresis, shows reproducibly a significant amount of crystallins, mainly alpha-crystallin. Evidence is provided that purified membranes from the bovine lens also associate selectively with limited number newly synthesized polypeptides on cell-free translation calf polyribosomes and addition to incubation medium. This capability retained membrane junctions. are...
In the calf eye lens, four morphologically distinct cell types can be detected: three in epithelial monolayer and one cortical part. During differentiation, there is a quantitative change synthesis of crystallin subunits. A marked increase alphaA-chains several beta-crystallin polypeptides accompanies transition from to fiberlike lens cells while non-crystallin proteins diminishes significantly.
The PC (proprotein convertase) furin cleaves a large variety of proproteins and hence plays major role in many pathologies. Therefore inhibition might be good strategy for therapeutic intervention, several inhibitors have been generated, although none are entirely furin-specific. To reduce potential side effects caused by cross-reactivity with other proteases, dromedary heavy-chain-derived nanobodies against catalytically active were developed as specific inhibitors. bound only to but not...
The effect of topical application the androgen 5 alpha-dihydrotestosterone (DHT), both encapsulated in liposomes and solved acetone, has been evaluated using female hamster flank organ as a model system. Systemic absorption DHT was significant from acetone solution, but negligible liposome biological is, however, proportionally diminished when system is used. Under experimental conditions used, lited had no advantages over this model.
Journal Article Evaluation of the hamster flank organ test for screening anti‐androgens Get access A.J.M. Vermorken, Vermorken Research Unit Cellular Differentiation and Transformation, University Nijmegen, The Netherlands Search other works by this author on: Oxford Academic Google Scholar C.M.A.A. Goos, Goos P. Wirtz Department Cytology Histology, British Dermatology, Volume 106, Issue 1, 1 January 1982, Pages 99–101, https://doi.org/10.1111/j.1365-2133.1982.tb00908.x Published: 01 1982
Upon addition of lens polyribosomes to a reticulocyte-cell-free system, alpha, beta L-, and gamma crystallin are synthesized, while H is not formed. This phenomenon comparable the biosynthetic events in lens-cell-free system tissue culture. It shown that formation depends upon presence polypeptide B1 b which arises by posttranslational modification. The putative precursor for BU messenger with sedimentation coefficient 12.5 S has been isolated.
In this study, a lateral flow immunochromatographic assay (LFIA) system for the detection of immunoglobulin M (IgM) antibodies, related to TORCH [(T)oxoplasmosis, (O)ther agents, (R)ubella (also known as German Measles), (C)ytomegalovirus, and (H)erpes simplex virus infections], based on gold magnetic nanoparticles, was established. Following modification with poly(methacrylic acid), nanoparticles conjugated an anti‑human IgM antibody (μ‑chain specific) construct probe. A device constructed...
Curcumin, a polyphenol extracted from turmeric (Curcuma longa), has emerged as potent multimodal cancer-preventing agent. It may attenuate the spread of cancer and render chemotherapy more effective. However, curcumin is neither well absorbed nor retained in blood, resulting low efficacy. In an attempt to enhance potency improve bioavailability curcumin, new delivery agents, hydroxypropyl-beta-cyclodextrin (HP-β-CD)-modified GoldMag nanoparticles (CD-GMNs) were designed synthesized...
The significance of diet for disease prevention has long been recognised. Dietary recommendations have therefore integrated in health promotion messages. Gastrointestinal functioning is essential the digestion nutrients. Oxidative stress observed patients with constipation, as well those colorectal cancer, cardiovascular and other chronic illnesses associated constipation. coexistence neoplasia coronary artery incriminated exposure to common risk factors increased oxidative stress. It was...
Proprotein convertases are serine endoproteases implicated in the proteolytic processing of a large variety regulatory proteins. An important role proprotein tumorigenic processes has been suggested by various studies. In this study, convertase furin PLAG1 proto-oncogene-induced salivary gland tumorigenesis was investigated. overexpression glands previously shown to result tumors 100% mice within 5 weeks after birth. MMTV-cre-mediated inactivation fur without over-expression caused smaller...
Progesterone, topically applied, prevents the stimulation of hamster flank organ by testosterone, but not dihydrotestosterone. 5 alpha-Dihydroprogesterone does prevent either two androgenic hormones. Neither progesterone nor alpha-dihydroprogesterone stimulate to any extent. Implications on use in acne therapy are discussed.
Methods using fluorogenic peptide substrates have been proposed for screening of proprotein convertase (PC) inhibitors and they are attractive since offer the advantage being sensitive, cost-effective susceptible to miniaturization. Several polyphenols, including epigallocatechin gallate ((-)EGCG), main component green tea, quercetin, widely distributed in fruit vegetables, however, led false positive results when were used. Processing genuine furin was not inhibited by these polyphenols. In...