A5003273710- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- interferon and immune responses
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Biochemical and Structural Characterization
- Invertebrate Immune Response Mechanisms
- Protein Hydrolysis and Bioactive Peptides
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Chemical Synthesis and Analysis
- vaccines and immunoinformatics approaches
- Infant Nutrition and Health
- Protease and Inhibitor Mechanisms
- Neuropeptides and Animal Physiology
- HIV Research and Treatment
- Force Microscopy Techniques and Applications
- Neonatal Respiratory Health Research
- Immune cells in cancer
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Machine Learning in Bioinformatics
- Bioinformatics and Genomic Networks
- Inflammasome and immune disorders
- RNA modifications and cancer
UPMC Hillman Cancer Center
2020-2024
University of Pittsburgh
2020-2024
Central Drug Research Institute
2017-2024
Institute of Structural and Molecular Biology
2018
University of Lucknow
2017
Abstract To become clinically effective, antimicrobial peptides (AMPs) should be non-cytotoxic to host cells. Piscidins are a group of fish-derived AMPs with potent and antiendotoxin activities but limited by extreme cytotoxicity. We conjectured that introduction cationic residue(s) at the interface polar non-polar faces piscidins may control their insertion into hydrophobic mammalian cell membrane thereby reducing have designed several novel analogs piscidin-1 substituting threonine L...
Abstract Marine fish antimicrobial peptide, chrysophsin-1 possesses versatile biological activities but its non-selective nature restricts therapeutic possibilities. Often small alterations in structural motifs result significant changes the properties of concerned proteins/peptides. We have identified GXXXXG motif chrysophsin-1. Glycine residue(s) this Chrysophsin-1 was/were replaced with alanine, valine and proline residue(s). Of these, proline-substituted analogs exhibited significantly...
A technology for systemic and repeated administration of osteogenic factors orthopedic use is an unmet medical need. Lactoferrin (∼80 kDa), present in milk, known to support bone growth. We discovered a lactoferrin-mimetic peptide, LP2 (an 18-residue fragment from the N-terminus N-lobe human lactoferrin), which self-assembles into nano-globular assembly with β-sheet structure aqueous environment. non-hemolytic non-cytotoxic against red blood cells 3T3 fibroblasts, respectively, appreciably...
Over the last few decades, anticancer peptides (ACPs) have turned into potential warheads against cancer. Apart from small molecules and monoclonal antibodies, ACPs been proven to be effective cancer cells. are cationic that selectively bind negatively charged cell membrane kill them by various mechanisms. In present study, we prepared a random scrambled library of 1200 100 known virtually screened for their properties. From in silico-predicted ACPs, 27 were prioritized based on support...
Abstract Lysosome has been long understood as a vital digestive organelle. Increasing reports indicate that the lysosome also plays crucial role in pathogenesis of variety neurodegenerative diseases, including Huntington's disease, Alzheimer's Parkinson's disease and amyotrophic lateral sclerosis. Abnormal protein degradation deposition stimulated by lysosomal dysfunction may cause age‐related neurodegeneration. Enormous efforts have devoted to development effective therapeutics against most...
Myeloid differentiation factor 2 (MD2), the TLR4 coreceptor, has been shown to possess opsonic activity and implicated in phagocytosis intracellular killing of Gram-negative bacteria. However, any MD2 protein segment involved bacteria is not yet known. A short synthetic segment, MD54 (amino acid regions 54 69), was interact with a bacterial outer membrane component, LPS, earlier. Furthermore, peptide induced aggregation LPS facilitated its internalization THP-1 cells. Currently, it...
Antimicrobial peptides are promising molecules in uprising consequences of drug-resistant bacteria. The prodomain furin, a serine protease, expressed all vertebrates including humans, is known to be important for physiological functions. Here, potent antimicrobial were mapped by extensive analyses overlapping peptide fragments the human furin. Two peptides, YR26 and YR23, active against bacterial cells MRSA-resistant Staphylococcus aureus epidermis 51625. Peptides largely devoid hemolytic...
Aβ29-40 residues with tryptophan in place of the lone methionine residue and three arginine added to its C-terminus exhibited augmented antibacterial activities protected mice against a lethal dose LPS. The results show conversion segment into cell-selective antimicrobial/anti-endotoxin peptide nanostructure cation-π interaction.
Immunomodulatory variants that lead to the loss or gain of specific protein interactions often manifest only as organismal phenotypes in infectious disease. Here, we propose a network-based approach integrate genetic variation with structurally resolved human interactome network prioritize immunomodulatory COVID-19. We find that, addition pass genome-wide significance thresholds, at interface protein-protein interactions, even though they do not meet are equally immunomodulatory. The...
Abstract The translational arrest of protein synthesis is often a cellular response to the virus infection. However, some antiviral proteins continue be translated through unknown mechanisms during this shutdown. Here, we report mechanism by which effectors are upregulated We found that interferon (IFN) stimulated gene, Oligoadenylate Synthetase 1 (OAS1), binds AU-rich elements (ARE) specific mRNA, including IFNβ, prolonging half-life and continued expression. This increased IFN expression...
Oligoadenylate synthetases (OAS) are a family of interferon (IFN)-stimulated genes known to inhibit viral replication through the enzymatic synthesis 2'-5' oligoadenylates and activation Ribonuclease L. However, this canonical mechanism does not explain antiviral properties enzymatically inactive OAS proteins. Here we describe an enzyme activity-independent function OAS1 that restricts West Nile virus (WNV) growth. The human P46 isoform, generated by alternative splicing G allele rs10774671...
Abstract Interferons inhibit virus replication through the expression of interferon stimulated genes (ISGs). We have found that a specific isoform one such ISG, Oligoadenylate Synthetase 1 (OAS1) limits host susceptibility to West Nile Virus (WNV) infection non-canonical mechanism. This OAS1 (OAS1 P46) in humans is generated due an alternative splice acceptor site at C-terminus gene. The SNP rs10774671 this has been associated with disease severity WNV. show human OAS1-KO cells lower basal...