A5018641343- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Platelet Disorders and Treatments
- Immunotherapy and Immune Responses
- Hemoglobinopathies and Related Disorders
- Blood disorders and treatments
- Hematopoietic Stem Cell Transplantation
- Neonatal Health and Biochemistry
- Advanced Adaptive Filtering Techniques
- Music and Audio Processing
- Cytomegalovirus and herpesvirus research
- Glycosylation and Glycoproteins Research
- Diabetes and associated disorders
- Speech and Audio Processing
- Autophagy in Disease and Therapy
- IL-33, ST2, and ILC Pathways
- Nitric Oxide and Endothelin Effects
- Neurological Disease Mechanisms and Treatments
- Chemokine receptors and signaling
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Genomics and Rare Diseases
- Genetic Syndromes and Imprinting
Institute for Infocomm Research
2025
Agency for Science, Technology and Research
2025
University of Nottingham Ningbo China
2016-2024
Yancheng Central Blood Station
2019-2021
Blood Center of Zhejiang Province
2009-2018
Shanghai Blood Center
2012-2014
Suzhou Vocational Health College
1987
The American Society for Histocompatibility and Immunogenetics HLA common well-documented (CWD) catalog, CWD 2.0.0 catalog European Federation (EFI) have been published, which are useful improving the accuracy of genotyping in laboratories. Here, we studied Chinese catalog. A total 812 211 unrelated volunteer donors from China Marrow Donor Program (CMDP) were analyzed. Six hundred seventy-six alleles at HLA-A, -B, -C, -DRB1, -DQB1 loci defined as population, including 159 517 alleles....
Immune thrombocytopenia (ITP) is an acquired and heterogeneous autoimmune-mediated hematological disease typically characterized by a low platelet count. Emerging evidence over the past several years suggests that biogenesis ageing are regulated, at least in part, apoptotic mechanisms. However, association between decreased platelets apoptosis ITP patients poorly understood. To better understand role of pathophysiology, we investigated markers from 40 chronic patients. Furthermore, results...
Summary The distribution of human leucocyte antigen (HLA) allele and haplotype is varied among different ethnic populations. In this study, HLA ‐A, ‐B ‐ DRB 1 frequencies were determined in 8333 volunteer bone marrow donors Zhejiang Han population using the polymerase chain reaction sequence‐based typing. A total 52 96 61 alleles found. Of these, top three frequent loci, respectively, A*11:01 (24.53%), A*24:02 (17.35%), A*02:01 (11.58%); B*40:01 (15.67%), B*46:01 (11.87%), B*58:01 (9.05%);...
In the present study, we have determined complete genomic sequence and analysed intron polymorphism of partial HLA-B HLA-C alleles in Chinese Han population. Over 3.0 kb DNA fragments loci were amplified by polymerase chain reaction from 5′ untranslated region to 3′ noncoding respectively, then products sequenced. Full-length nucleotide sequences 14 10 obtained been submitted GenBank IMGT/HLA database. Two novel HLA-B*52:01:01:02 HLA-B*59:01:01:02 identified, HLA-B*52:01:01:01 was firstly...
Immune thrombocytopenia (ITP) is an autoimmune disease characterized by a low platelet count and insufficient production. Previous studies identified that microRNAs (miRNAs/miRs) are important for function. However, the regulatory role of miRNAs in pathogenesis ITP remains unclear. The aim present study to isolate differentially expressed miRNAs, identify their roles platelets from ITP. A total 5 ml blood 22 patients with 8 healthy controls was isolated collection. microarray assay performed...
Abstract Major histocompatibility complex class I chain‐related gene A ( MICA ) is located 46 kb centromeric to HLA‐B locus and encodes a stress‐inducible protein. allelic variation thought be associated with disease susceptibility immune response transplants. In this study, polymerase chain reaction sequence‐based typing (PCR‐SBT) method for alleles has been established. Genomic DNAs from 100 healthy Chinese Han individuals were typed by method. The microsatellite polymorphism in the exon 5...
ABO grouping is the most important pretransfusion testing that directly related to safety of blood transfusion. A weak subgroup one causes an discrepancy. Here, we investigated characterization four novel variants including a B(A) subgroup.
To analyse the complete genomic sequences and investigate intron polymorphism of human leucocyte antigen (HLA)-A locus, full-length nucleotide each major allelic group HLA-A in Chinese Han population were determined, including HLA-A*01, A*02, A*03, A*11, A*23, A*24, A*26, A*29, A*30, A*31, A*32, A*33, A*34, A*68, A*69. More than 3.0-kb DNA fragment locus was amplified from 5'-untranslated region to 3'-noncoding for sequencing. Full-length alleles determined using an ABI BigDye((R))...
Currently for the majority of HLA-DRB1 alleles focus has been mainly on exon 2 and complete cDNA sequences are rare. In this study, we analyzed coding partial locus. The was amplified by polymerase chain reaction using group-specific primers located in 5'- 3'-untranslated regions to obtain sequences. amplification products were sequenced an ABI BigDye® Terminator Cycle Sequencing kit. allele phylogenetic tree dnaman software. Full-length 22 obtained study. HLA-DRB1*08:09, DRB1 *12:02:01,...
The para-Bombay phenotype is characterised by H-deficient or H partially deficient red blood cells (RBCs) in individuals who secrete ABH antigens their saliva. Samples from an individual whose RBCs had apparent and his family members were investigated a novel FUT1 allele was identified.RBCs' standard serologic technique. Genomic DNA sequenced with primers that amplified the coding sequence of FUT2, respectively. Routine ABO genotyping analysis performed. Haplotypes identified TOPO cloning...
Summary The DNA ‐based method is used widely for HLA genotyping in routine work, but some allele may be dropout the procedure. Here, we reported a case with ‐ A S anger PCR SBT test. initial commercial agent kit was not characterized, and result of L uminex technology indicated as *02 allele. Subsequently, sequences exons 2–4 were fully matched *02:07 *11:01:01 by group‐specific primer amplification . On further analysis, novel *02:07:07 identified, which has one nucleotide difference from...
Background Although more than 80 weak D types have been reported, many rare alleles probably remain unidentified. However, direct evidence that associates intronic mutations in the RHD gene with is lacking. Study Design and Methods Blood samples were obtained from S hanghai B lood C enter. – typed routine laboratories tested using a monoclonal immunoglobulin M reagent, an indirect antiglobulin test, commercial panel of anti‐ ( iagast ‐ creen). nucleotide sequencing included adjacent flanking...
The Diego blood group system plays an important role in transfusion medicine. Genotyping of DI1 and DI2 alleles is helpful for the investigation into haemolytic disease newborn (HDN) development rare databases. Here, we set up a polymerase chain reaction sequence-based typing (PCR-SBT) method genotyping alleles.Specific primers exon 19 solute carrier family 4, anion exchanger, member1 (SLC4A1) gene were designed, our PCR-SBT was established optimized genotyping. A total 1053 samples from...
Summary Currently, Luminex technology based on the PCR sequence‐specific oligonucleotide (SSO) probe method has been widely used for HLA genotyping in immunogenetics laboratories. Here, we reported a case with HLA‐B allele dropout by technology. The initial result of commercial agent kit was inconclusive, and then, PCR‐SBT indicated as HLA‐B*58 allele. Subsequently, full‐length sequence determined TOPO‐TA cloning, novel B*58:01:01:02 identified individual. Compared HLA‐B*58:01:01:01, showed...
Here, we report genomic full-length sequence of a novel HLA-A*11:01:01:02 allele identified in Chinese individual. has three nucleotide differences from HLA-A*11:01:01:01, including 99 C>G intron 1, 655 C>T and G deletion position 656 2.
HLA-A*11:57 allele was different from HLA-A*11:16 by single nucleotide substitution at codon 145(CAC>CGC), resulting in one amino acid change His to Arg.
The rapid advancements in large language models (LLMs) have significantly enhanced natural processing capabilities, facilitating the development of AudioLLMs that process and understand speech audio inputs alongside text. Existing typically combine a pre-trained encoder with LLM, which are subsequently finetuned on specific tasks. However, has constrained capacity to capture features for new tasks datasets. To address this, we propose incorporate mixtures `weak' encoders (MoWE) into AudioLLM...
Penetration testing is essential to ensure Web security, which can detect and fix vulnerabilities in advance, prevent data leakage serious consequences. The powerful inference capabilities of large language models (LLMs) have made significant progress various fields, the development potential LLM-based agents revolutionize cybersecurity penetration industry. In this work, we establish a comprehensive end-to-end benchmark using real-world environment explore domain. Our results reveal that...
We introduce MERaLiON-AudioLLM (Multimodal Empathetic Reasoning and Learning in One Network), the first speech-text model tailored for Singapore's multilingual multicultural landscape. Developed under National Large Language Models Funding Initiative, Singapore, integrates advanced speech text processing to address diverse linguistic nuances of local accents dialects, enhancing accessibility usability complex, environments. Our results demonstrate improvements both recognition task-specific...
To screen rare blood groups Fy(a-), s-, k-, Di(b-) and Js(b-) in an ethnic Zhuang population.Sequence-specific primers were designed based on single nucleotide polymorphism (SNP) sites of group antigens Fy(b) s. A specific multiplex PCR system I was established. Multiplex II applied to detect alleles Di(b), k, Js(b)1910 Js(b) 2019 at the same time. The two systems used for 4490 randomly selected healthy donors Guangxi origin.We successfully made I. We detected using system. There are five...
The authors have disclosed no conflicts of interest.