A5107519282- Adenosine and Purinergic Signaling
- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Neuroendocrine regulation and behavior
- Nicotinic Acetylcholine Receptors Study
- Medicinal plant effects and applications
- Tryptophan and brain disorders
- Cannabis and Cannabinoid Research
- Biochemical Analysis and Sensing Techniques
- Drug Transport and Resistance Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Substance Abuse Treatment and Outcomes
- Biological Activity of Diterpenoids and Biflavonoids
- Renal function and acid-base balance
- Ion channel regulation and function
- Obsessive-Compulsive Spectrum Disorders
- Diet and metabolism studies
- Alcohol Consumption and Health Effects
- Gut microbiota and health
- Natural product bioactivities and synthesis
- Amino Acid Enzymes and Metabolism
- Photoreceptor and optogenetics research
- Prenatal Substance Exposure Effects
University of Southern California
2015-2024
Southern California University for Professional Studies
2004-2020
Royal Free London NHS Foundation Trust
2020
University of California, Los Angeles
2017
Royal Liverpool University Hospital
2015
University of Liverpool
2015
Stanford University
2011-2014
Stanford Medicine
2012-2014
University of Pittsburgh
2014
Amgen (United States)
2014
The depletion of striatal dopamine (DA) that can occur after methamphetamine (METH) administration has been linked to METH-induced hyperthermia. relationship between hyperthermia, neurotoxicity (striatal DA depletions) and compounds protect against METH was further investigated in this study. Typically, rats exposed die when their body temperatures exceed 41.3 degrees C but such hyperthermic be saved by hypothermic intervention. Subsequently, intervention have greater at an earlier time...
The macrophage is a major phagocytic cell type, and its impaired function primary cause of immune paralysis, organ injury, death in sepsis. An incomplete understanding the endogenous molecules that regulate bactericidal activity barrier for developing effective therapies Using an vitro killing assay, we report here purine ATP augments sepsis-causing bacteria by macrophages through P2X4 receptors (P2X4Rs). newly developed transgenic mice expressing bioluminescent probe on surface, found...
Anxiety disorders are the most prevalent mental illnesses in U.S. and estimated to consume one-third of country's health treatment cost. Although anxiolytic therapies available, many patients still exhibit resistance, relapse, or substantial side effects. Further, due COVID-19 pandemic stay-at-home order, social isolation, fear pandemic, unprecedented times, incidence anxiety has dramatically increased. Previously, we have demonstrated dihydromyricetin (DHM), major bioactive flavonoid...
Background Excess alcohol (ethanol, EtOH) consumption is a significant cause of chronic liver disease, accounting for nearly half the cirrhosis‐associated deaths in United States. EtOH‐induced toxicity linked to EtOH metabolism and its associated increase proinflammatory cytokines, oxidative stress, subsequent activation Kupffer cells. Dihydromyricetin (DHM), bioflavonoid isolated from Hovenia dulcis , can reduce intoxication potentially protect against chemical‐induced injuries. But there...
P2X4 receptors are ATP-gated cation channels that widely expressed in the nervous system. To identify receptor-expressing cells, we generated BAC transgenic mice expressing tdTomato under control of receptor gene (<i>P2rx4</i>). We found sparse populations tdTomato-positive neurons most brain areas with patterns matched mRNA distribution. expression within microglia was low but increased by an experimental manipulation triggered microglial activation. surprisingly high hypothalamic arcuate...
Excessive alcohol consumption leads to a myriad of detrimental health effects, including alcohol-associated liver disease (ALD). Unfortunately, no available treatments exist combat the progression ALD beyond corticosteroid administration and/or transplants. Dihydromyricetin (DHM) is bioactive polyphenol and flavonoid that has traditionally been used in Chinese herbal medicine for its robust antioxidant anti-inflammatory properties. It derived from many plants, Hovenia dulcis found as active...
Abstract Considerable evidence indicates that ethanol acts on specific residues in the transmembrane domains of glycine receptors (GlyRs). In this study, we tested hypothesis extracellular domain is also a target for action by investigating effect cysteine substitutions at positions 52 (extracellular domain) and 267 (transmembrane responses to n ‐alcohols propyl methanethiosulfonate (PMTS) α1GlyRs expressed Xenopus oocytes. support hypothesis: (i) The A52C mutation changed sensitivity...
Purinergic P2X4 receptors (P2X4Rs) belong to the P2X superfamily of ion channels regulated by ATP. We recently demonstrated that P2X4R knockout (KO) mice exhibited deficits in sensorimotor gating, social interaction, and ethanol drinking behavior. Dopamine (DA) dysfunction may underlie these behavioral changes, but there is no direct evidence for P2X4Rs' role DA neurotransmission. To test this hypothesis, we measured markers function dependent behaviors KO mice. altered density pre-synaptic...
ATP-gated purinergic P2X4 receptors (P2X4Rs) are expressed in the central nervous system and sensitive to ethanol at intoxicating concentrations. P2XRs trimeric; each subunit consists of two transmembrane (TM) α-helical segments, a large extracellular domain, intracellular amino carboxyl terminals. Recent work indicates that position 336 (Met336) TM2 segment is critical for modulation P2X4Rs. The anthelmintic medication ivermectin (IVM) positively modulates P2X4Rs believed act same region as...
Purinergic ionotropic P2X receptors are a family of cation-permeable channels that bind extracellular adenosine 5'-triphosphate. In particular, convergent lines evidence have recently highlighted P2X(4) as potentially critical target in the regulation multiple nervous and behavioural functions, including pain, neuroendocrine hippocampal plasticity. Nevertheless, role receptor organization remains poorly investigated. To study effects activation, we tested acute its potent positive allosteric...
P2X receptors (P2XRs) are ion channels gated by synaptically released ATP. The P2X4 is the most abundant P2XR subtype expressed in central nervous system and to date ethanol-sensitive. In addition, genomic findings suggest that P2X4Rs may play a role alcohol intake/preference. However, little known regarding how ethanol causes inhibition of ATP-gated currents P2X4Rs. We begin address this issue investigating effects wild-type mutant D331A M336A human embryonic kidney (HEK) 293 cells using...