A5036601296- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Pelvic and Acetabular Injuries
- Pelvic floor disorders treatments
- SARS-CoV-2 detection and testing
- Neuroendocrine regulation and behavior
- RNA Research and Splicing
- Regulation of Appetite and Obesity
- Histone Deacetylase Inhibitors Research
- Biosensors and Analytical Detection
- Multiple Myeloma Research and Treatments
- Precipitation Measurement and Analysis
- Particle Dynamics in Fluid Flows
- Maternal and fetal healthcare
- Advanced Mathematical Theories and Applications
- Assisted Reproductive Technology and Twin Pregnancy
- Animal Genetics and Reproduction
- Ureteral procedures and complications
- Adipokines, Inflammation, and Metabolic Diseases
- Kruppel-like factors research
Washington University in St. Louis
2017-2022
Chan Zuckerberg Initiative (United States)
2020
The Korean Association for Public Administration
2019
Institute for Systems Biology
2019
Cork University Hospital
2016
The Open University
2008-2014
National Maternity Hospital
2013
Dalhousie University
1992-1997
McMaster University
1974
Abstract Background Rapid, reliable, and widespread testing is required to curtail the ongoing COVID-19 pandemic. Current gold-standard nucleic acid tests are hampered by supply shortages in critical reagents including nasal swabs, RNA extraction kits, personal protective equipment, instrumentation, labor. Methods To overcome these challenges, we developed a rapid colorimetric assay using reverse-transcription loop-mediated isothermal amplification (RT-LAMP) optimized on human saliva samples...
Abstract Rapid, reliable, and widespread testing is required to curtail the ongoing COVID-19 pandemic. Current gold standard nucleic acid tests are hampered by supply shortages in critical reagents including nasal swabs, RNA extraction kits, personal protective equipment (PPE), instrumentation, labor. Here we present an approach overcome these challenges with development of a rapid colorimetric assay using reverse-transcription loop-mediated isothermal amplification (RT-LAMP) optimized on...
Sex can be an important determinant of cancer phenotype, and exploring sex-biased tumor biology holds promise for identifying novel therapeutic targets new approaches to treatment. In established isogenic murine model glioblastoma (GBM), we discovered correlated transcriptome-wide sex differences in gene expression, H3K27ac marks, large Brd4-bound enhancer usage, Brd4 localization Myc p53 genomic binding sites. These expression patterns were also evident human stem cells (GSCs). observations...
The concept of mutually unbiased bases is studied for $N$ pairs continuous variables. To find reduces, specific states related to the Heisenberg-Weyl group, a problem symplectic geometry. Given single pair variables, three are identified while five such exhibited two For $N=2$, golden ratio occurs in definition these suggesting relevance number theory not only finite-dimensional setting.
The piggyBac (PB) transposon has been used in a number of biological applications. insertion PB transposons into the genome can disrupt genes or regulatory regions, impacting cellular function, so for many experiments it is important that transposition tightly controlled. Here, we systematically characterize three methods post-translational control four cell lines. We investigated fusions transposase with ERT2 and two degradation domains (FKBP-DD, DHFR-DD), multiple orientations, determined...
Transcription factors (TFs) enact precise regulation of gene expression through site-specific, genome-wide binding. Common methods for TF-occupancy profiling, such as chromatin immunoprecipitation, are limited by requirement TF-specific antibodies and provide only end-point snapshots TF Alternatively, TF-tagging techniques, in which a is fused to DNA-modifying enzyme that marks TF-binding events across the genome they occur, do not require offer potential unique applications, recording...
The excitatory amino-acid L-glutamate appears to be involved in the neural regulation of puberty. We have now characterized stimulatory effect glutamate agonist N-methyl-D-aspartic acid (NMDA) on secretion luteinizing hormone (LH) immature female rats. NMDA injections (subcutaneously) rapidly induce LH (maximum at 8 min) with an ED(50) 3.25 mg/kg determined postnatal day 30. response develops between 10 and 15 days after birth, reaches a maximum approximately 27 disappears again by 32, age...
Abstract In situ measurements of transcription factor (TF) binding are confounded by cellular heterogeneity and represent averaged profiles in complex tissues. Single cell RNA-seq (scRNA-seq) is capable resolving different types based on gene expression profiles, but no technology exists to directly link specific the pattern TFs those types. Here, we present self-reporting transposons (SRTs) their use single calling cards (scCC), a novel assay for simultaneously capturing mapping TF sites...
Introduction: Although older individuals are more prone to hypoglycaemia, it is not known if they have sufficient understanding of the risks hypoglycaemia or factors that predispose it. We evaluated effectiveness education and examined increased susceptibility among people with diabetes. Methods: Forty-five patients (male/female) aged >65 years diabetes were identified through outpatient clinics at a secondary care hospital. Information relating received, awareness associated risk was...
This paper discusses the evolution of droplet size distribution for a liquid-in-gas aerosol contained in Rayleigh-Bénard cell. It introduces non-collisional model broadening distribution, termed "convective ripening". The also considers initiation rainfall from ice-free cumulus clouds. is argued that while collisional mechanisms cannot explain production rain clouds with water diameters , convective ripening mechanism gives much faster route to increasing small fraction droplets grow into raindrops.
Sex can be an important determinant of cancer phenotype, and exploring sex-biased tumor biology holds promise for identifying novel therapeutic targets new approaches to treatment. In established isogenic murine model glioblastoma, we discovered correlated transcriptome-wide sex differences in gene expression, H3K27ac marks, large Brd4-bound enhancer usage, Brd4 localization Myc p53 genomic binding sites. These expression patterns were also evident human glioblastoma stem cells (GSCs)....
Abstract Transcription factors (TFs) play a central role in the regulation of gene expression, controlling everything from cell fate decisions to activity dependent expression. However, widely-used methods for TF profiling vivo (e.g. ChIP-seq) yield only an aggregated picture binding across all types present within harvested tissue; thus, it is challenging or impossible determine how same might bind different portions genome types, even identify its events at rare complex tissue such as...
A method is described for the preparation and growth of rat pineal monolayer cultures derived from both mature immature animals. The were observed to undergo profound but reversible morphological differentiations by addition dibutyryl-cAMP, monobutyryl-cAMP, papaverine, prostaglandins, adenosine, removal serum. Sodium butyrate theophylline had no effect. Time-lapse photography indicated that this transformation took place via a contraction-relaxation mechanism. Both colcemid cytochalasin B...
Single-cell RNA sequencing is a powerful tool for exploring gene expression heterogeneity, but the results may be obscured by technical noise inherent in experimental procedure. Here we introduce novel parametrisation of sc-RNA data, giving estimates probability activation and its peak transcription rate, which are agnostic about mechanism underlying fluctuations counts. Applying this approach to single cell mRNA counts across different tissues adult mice, find that levels approximately...
This protocol describes how to create calling card libraries from bulk RNA. assumes you have successfully transformed cells with piggyBac self-reporting transposons and either undirected transposase or your favorite transcription factor (YFTF) fused piggyBac. Your are now ready for RNA extraction, SRT amplification, library preparation.
Transposon calling cards can identify transcription factor (TF) binding sites. This involves fusing your favorite TF (YFTF) to the hyperactive piggyBac transposase (HyPBase). is delivered cells in conjunction with a transposon. The will visit sites genome and YFTF-HyPBase deposit transposons near We then generate sequencing libraries map genome-wide localization of transposons. Finally, we significant clusters insertions