A5017327952- Gut microbiota and health
- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Cholesterol and Lipid Metabolism
- Protein Structure and Dynamics
- Aortic aneurysm repair treatments
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Diet and metabolism studies
- Metabolism and Genetic Disorders
- Livestock and Poultry Management
- Animal Nutrition and Physiology
- Cardiac and Coronary Surgery Techniques
- Peroxisome Proliferator-Activated Receptors
- Aortic Disease and Treatment Approaches
- Adsorption and Cooling Systems
- Sphingolipid Metabolism and Signaling
- Mycobacterium research and diagnosis
- Advanced Glycation End Products research
- Peripheral Artery Disease Management
- Atherosclerosis and Cardiovascular Diseases
- Diet, Metabolism, and Disease
- Cynara cardunculus studies
- Liver Disease Diagnosis and Treatment
- Machine Learning in Bioinformatics
- Graphene and Nanomaterials Applications
Durham VA Medical Center
2019
Mission Hospital
2019
Medical University of Graz
2017-2019
Mountain Area Health Education Center
2019
Cleveland Clinic Lerner College of Medicine
2018
Duke Medical Center
2017-2018
Duke University
2018
Cleveland Clinic
2009-2017
University of North Dakota
2016
Sanford Health
2016
Myeloperoxidase (MPO) and paraoxonase 1 (PON1) are high-density lipoprotein-associated (HDL-associated) proteins mechanistically linked to inflammation, oxidant stress, atherosclerosis. MPO is a source of ROS during inflammation can oxidize apolipoprotein A1 (APOA1) HDL, impairing its atheroprotective functions. In contrast, PON1 fosters systemic antioxidant effects promotes some the properties attributed HDL. Here, we demonstrate that MPO, PON1, HDL bind one another, forming ternary...
Summary Background Faecal microbiota transplantation is an experimental approach for the treatment of patients with ulcerative colitis. Although there growing evidence that faecal effective in this disease, factors affecting its response are unknown. Aims To establish a protocol colitis patients, and to investigate which patient or donor responsible success. Methods This open controlled trial repeated after antibiotic pre‐treatment ( FMT ‐group, n = 17) vs only AB 10) 27 therapy refractory...
Using an untargeted metabolomics approach in initial (N = 99 subjects) and replication cohorts 1,162), we discovered structurally identified a plasma metabolite associated with cardiovascular disease (CVD) risks, N6,N6,N6-trimethyl-L-lysine (trimethyllysine, TML). Stable-isotope-dilution tandem mass spectrometry analyses of independent validation cohort 2,140) confirmed TML levels are independently incident (3-year) major adverse event risks (hazards ratio [HR], 2.4; 95% CI, 1.7-3.4)...
High density lipoprotein (HDL), the carrier of so-called “good” cholesterol, serves as major athero-protective and has emerged a key therapeutic target for cardiovascular disease. We applied small angle neutron scattering (SANS) with contrast variation selective isotopic deuteration to study nascent HDL obtain low resolution structure in solution overall time-averaged conformation apolipoprotein AI (apoA-I) versus lipid (acyl chain) core particle. Remarkably, apoA-I is observed possess an...
Tongue weakness, like all weakness in Duchenne muscular dystrophy (DMD), occurs as a result of contraction-induced muscle damage and deficient repair. Although membrane fragility is known to potentiate injury DMD, whether stem cells are implicated repair remains unclear. We hypothesized that DMD myoblasts less sensitive cues the extracellular matrix designed structure-function relationships healthy muscle. To test this hypothesis, we drew inspiration from tongue engineered contractile human...
Background The optimal surgical approach for management of acute type A aortic dissection remains controversial. This study aimed to assess outcomes reoperation after repair help guide decision making around index operative strategy. Methods and Results All reoperations (n=129) at a single referral institution from August 2005 April 2016 prior were reviewed. primary outcome was 30‐day or in‐hospital mortality. Secondary included organ‐specific morbidity 1‐ 5‐year as estimated using the...
Spherical high density lipoprotein (sHDL), a key player in reverse cholesterol transport and the most abundant form of HDL, is associated with cardiovascular diseases. Small angle neutron scattering contrast variation was used to determine solution structure protein lipid components reconstituted sHDL. Apolipoprotein A1, major sHDL, forms hollow that cradles central compact core. Three apoA1 chains are arranged within low resolution component as one three possible global architectures: (i)...
Persister cells are a subpopulation of bacteria capable surviving high doses antibiotics and various stressors, contributing to chronic infections antibiotic resistance. Ethanol exposure is known induce significant morphological physiological changes, such as alterations in fatty acid composition, ion leakage, increased membrane fluidity, which may promote biofilm formation. In the current study, Escherichia coli Staphylococcus epidermidis were cultured LB broth supplemented with varying...
Increased circulating levels of apolipoprotein A-I (apoA-I), the major protein high-density lipoprotein (HDL), by genetic manipulation or infusion, protects against melanoma growth and metastasis. Herein, we explored potential roles in tumorigenesis for host scavenger receptor class B, type 1 (SR-B1), ATP-binding cassette transporters A1 (ABCA1) G1 (ABCG1), all mediators apoA-I HDL sterol lipid transport function. In a syngeneic murine tumor model, B16F10, mice with global deletion SR-B1...
Protein structural annotation and classification is an important challenging problem in bioinformatics. Research towards analysis of sequence–structure correspondences critical for better understanding a protein's structure, function, its interaction with other molecules. Clustering protein domains based on their similarities provides valuable information schemes. In this article, we attempt to determine whether structure alone sufficient adequately classify structures. We present algorithm...
Small-angle neutron scattering (SANS) with contrast variation was used to obtain the low-resolution structure of nascent HDL (nHDL) reconstituted dimyristoyl phosphatidylcholine (DMPC) in absence and presence cholesterol, [apoA1:DMPC (1:80, mol:mol) apoA1:DMPC:cholesterol (1:86:9, mol:mol:mol)]. The overall shape both particles is discoidal apoA1 visualized as an open, contorted, out plane conformation three arms HDL/dimyristoyl without cholesterol (nHDLDMPC) two (nHDLDMPC+Chol). lipid phase...
This work examines pluronic F-127 poloxamer for cell protection during injection through a syringe needle. Direct is minimally invasive method transplantation; however, it often results in poor viability. We proposed that encapsulating cells this hydrogel would protect from detrimental mechanical forces and increase The was tested at multiple weights carbon nanobrush concentrations to determine how gel weight affects viability as well allow the gels remain electrically conductive scaffolds....
Background: A healthy 21 year old female developed severe fatigue and peripheral edema in the upper extremities after an extreme exercise workout session. 4 ½ months later she participated a similar session with personal trainer experienced same symptoms.