A5044676253- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- DNA and Nucleic Acid Chemistry
- Protein Kinase Regulation and GTPase Signaling
- Medical Imaging Techniques and Applications
- Immune cells in cancer
- Medical Imaging and Pathology Studies
- Bone health and treatments
- Bacteriophages and microbial interactions
- Radiopharmaceutical Chemistry and Applications
- Immune Response and Inflammation
- Heat shock proteins research
- Radiation Effects and Dosimetry
- Plant Virus Research Studies
- Protein Structure and Dynamics
- Viral gastroenteritis research and epidemiology
- Viral Infections and Outbreaks Research
- Nuclear Receptors and Signaling
- Advanced X-ray Imaging Techniques
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Electrochemical Analysis and Applications
- Advanced MRI Techniques and Applications
- Thermoregulation and physiological responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Melanoma and MAPK Pathways
National Defense Medical College
2023-2025
United States Army
2023
United States Army Medical Command
2021
Northeastern University
2013-2019
North Carolina State University
2011-2019
Scripps Research Institute
2014-2015
Various miniature Clark-type oxygen electrodes (COEs), which are typically used to measure dissolved (DO) concentration in cellular respiration, have been developed since the 1980s. Arrays with individually addressable that constitute sensor were for various applications. However, large number of leads and contact pads required connecting external instrument complicate electrode layout make operation integrated COE arrays challenging. Here, we fabricated closed bipolar electrochemical...
Heat-related illnesses cause multiple organ injuries, including acute kidney injury (AKI). Recent studies have reported that heat-induced AKI can progress to chronic disease (CKD). We previously urinary levels of liver fatty acid-binding protein (L-FABP) are elevated in patients with severe heat-related illness. In this study, we prospectively examined the detection ability L-FABP rapid assay kit (L-FABP Point-of-Care [POC] kit) for damage prehospital settings. After applying exclusion...
IntroductionRepeated injections of low-dose lipopolysaccharide (LPS preconditioning) augment the antibacterial activity liver macrophages. In this study, a mouse model acute kidney injury (AKI) induced by Staphylococcus aureus (S. aureus) bacteremia was used to investigate effects LPS preconditioning on renal macrophages.MethodsEight-week-old C57BL/6J mice were preconditioned with either (5 μg/kg) or vehicle for three consecutive days. Kidney immune cells isolated, and macrophages assessed...
A major complication of heat-related illness is the development acute kidney injury (AKI) and damage to tubular cells. Because cells use fatty acids as a energy source, impaired acid oxidation (FAO) may be associated with due heat stress. Carnitine essential in transportation into mitochondria for FAO. To date, there has been little attention given role carnitine AKI. evaluate relationship between inadequacy severity or AKI, we examined serum levels patients illness. We also used...
Abstract The mortality rate for acute kidney injury (AKI) due to sepsis remains high, and effective therapies based on its pathogenesis remain elusive. Macrophages are crucial clearing bacteria from vital organs, including the kidney, under septic conditions. Excessive macrophage activation results in organ injury. C-reactive protein (CRP) peptide (174-185), a functional product of proteolyzed CRP vivo, effectively activates macrophages. We investigated therapeutic efficacy synthetic AKI,...
Abstract Liver macrophages are critical components of systemic immune system defense mechanisms. F4/80 high Kupffer cells (KCs) the predominant liver‐resident and first to contact pathogens entering liver. low monocyte‐derived (MoMφs) essential that modulate liver functions. Here we report a novel method identifying subpopulations these two populations using traditional flow cytometry examine each subpopulation for its putative roles in pathogenesis an experimental non‐alcoholic...
Quasi‐equivalent viruses that infect animals and bacteria require a maturation process in which particles transition from initially assembled procapsids to infectious virions. Nudaurelia capensis ω virus (NωV) is T = 4, eukaryotic, single‐stranded ribonucleic acid has proved be an excellent model system for studying the mechanisms of viral maturation. Structures NωV (diameter 480 Å), intermediate (410 mature virion Å) were determined by electron cryo‐microscopy three‐dimensional image...
Water and ligand binding play critical roles in the structure function of proteins, yet their sites significance are difficult to predict a priori. Multiple solvent crystal structures (MSCS) is method where several X-ray solved, each unique environment, with organic molecules that serve as probes protein surface for evolved bind ligands, while first hydration shell essentially maintained. When superimposed, these contain vast amount information regarding hot spots protein-protein or...
Atomic models of small icosahedral RNA viruses were first derived from X-ray crystal structures between 1978 and 1990. In that same period, electron cryo-microscopy with image reconstructions (cryoEM) was being developed, among the early successful subjects study. By 1990 it clear many important virus-related (e.g. antibodies receptor molecules bound to viruses) would be difficult or impossible crystalize but could imaged by cryoEM at low moderate resolution. Combining Fab fragments, virus...