A5100372269- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- Bone Tissue Engineering Materials
- Medical Imaging and Pathology Studies
- Immune Response and Inflammation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Autophagy in Disease and Therapy
- Cancer-related molecular mechanisms research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Parasites and Host Interactions
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Orthopaedic implants and arthroplasty
- Phagocytosis and Immune Regulation
- Occupational and environmental lung diseases
- MicroRNA in disease regulation
- Lung Cancer Diagnosis and Treatment
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer, Hypoxia, and Metabolism
- COVID-19 Clinical Research Studies
- Dental Implant Techniques and Outcomes
- Ferroptosis and cancer prognosis
- Mesenchymal stem cell research
- Analytical chemistry methods development
- Reproductive tract infections research
Affiliated Eye Hospital of Wenzhou Medical College
2025
Wenzhou Medical University
2025
Beijing National Laboratory for Molecular Sciences
2025
University of Chinese Academy of Sciences
2025
Tianjin Medical University
2014-2024
Tianjin haihe hospital
2013-2024
Tianjin University
2017-2024
Sichuan University
2010-2024
West China Hospital of Sichuan University
2024
Tianjin Medical University General Hospital
2024
Progressive tissue fibrosis is a cause of major morbidity and mortality. Pulmonary an epithelial-mesenchymal disorder in which TGF-β1 plays central role pathogenesis. Here we show that follistatin-like 1 (FSTL1) differentially regulates TGF-β bone morphogenetic protein signaling, leading to epithelial injury fibroblast activation. Haplodeletion Fstl1 mice or blockage FSTL1 with neutralizing antibody reduced bleomycin-induced vivo. induced response lung promotes the accumulation...
Osteogenesis of mesenchymal stem cells (MSCs) is essential for bone repair. Recently, microRNAs have been proven to play an important role in the regulation MSC differentiation, including osteogenesis. Here, function microRNA-21 (miR-21) osteogenic differentiation human umbilical cord (hUMSCs) was investigated. Briefly, miR-21 mimics (m-miR-21) and antisense (as-miR-21) were transfected hUMSCs, capacity hUMSCs evaluated by expression markers encoding alkaline phosphatase (ALP), runt-related...
Silver nanoparticles are increasingly recognized for their utility in biological applications, especially antibacterial effects. Herein, we confirmed the effect of silver on Escherichia coli using conventional optical density (OD) and colony-forming units (CFU) method used flow cytometry (FC), TEM BrdU ELISA to investigate mechanisms this effect. From results, conclude that AgNPs can simultaneously induce apoptosis inhibit new DNA synthesis cells a positive concentration-dependent manner....
Abstract Efficient repair of injured epithelium by airway progenitor cells could prevent acute inflammation from progressing into chronic phase in lung. Here, we used small molecules, genetic loss-of-function, organoid cultures, and vivo lung-injury models to show that autophagy is essential for maintaining the pool stem-like vClub promoting their proliferation during ovalbumin-induced inflammation. Mechanistically, impaired disrupted glucose uptake cells, either reduced accessibility or...
Autophagy is a protective cellular mechanism in response to stress conditions. However, whether autophagy required for maintenance of the alveolar epithelium unknown. Here, we report that loss autophagy-related 5 (Atg5) AT2 cells worsened bleomycin-induced lung injury. Mechanistically, during bleomycin injury, downregulated lipid metabolism but upregulated glucose repair. Chemical blockade fatty acid synthesis promoted organoid growth and counteracted effects on genetic transporter 1,...
Abstract Coronavirus disease 2019 (COVID-19) has gained prominence as a global pandemic. Studies have suggested that systemic alterations persist in considerable proportion of COVID-19 patients after hospital discharge. We used proteomic and metabolomic approaches to analyze plasma samples obtained from 30 healthy subjects 54 survivors 6 months discharge the hospital, including non-severe 24 severe patients. Through this analysis, we identified 1019 proteins 1091 metabolites. The...
Objective This retrospective study aims to evaluate the therapeutic effect of varying dosages adjuvant radioactive iodine (RAI) therapy on intermediate-risk papillary thyroid carcinoma (PTC) patients. Methods involved a total 427 PTC patients, out which 202 received 3.7GBq dosage RAI, and 225 5.55GBq dosage. The evaluation assessing outcomes, number treatment cycles, successful remnant ablation rates in both dose groups, six months post-adjuvant RAI therapy. Univariate multivariate logistic...
Omicron variants of SARS-CoV-2 have spread rapidly worldwide; however, most infected patients mild or no symptoms. This study aimed to understand the host response infections by performing metabolomic profiling plasma. We observed that triggered an inflammatory and innate immune, adaptive immunity was suppressed, including reduced T-cell immunoglobulin antibody production. Similar original strain circulating in 2019, developed anti-inflammatory accelerated energy metabolism infection....
(1) Background: Abnormal repair after alveolar epithelial injury drives the progression of idiopathic pulmonary fibrosis (IPF). The maintenance integrity is based on self-renewal and differentiation type 2 (AT2) cells, which require sufficient energy. However, role glutamine metabolism in epithelium remains unclear. In this study, we investigated AT2 cells patients with IPF mice bleomycin-induced fibrosis. (2) Methods: Single-cell RNA sequencing (scRNA-seq), transcriptome, metabolomics...
The present study aims to investigate the contribution of two biologically important cations, Mg 2+ and Sr , when co-substituted into structure hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 HA). substituted samples were synthesized by a hydrothermal method that involved addition containing precursors partially replace Ca in apatite structure. Four HA with different concentrations ((Mg + Sr)/(Mg Ca) = 30%) investigated, they compared pure HA. Experimental results showed only limited amount (Mg/(Mg...
BackgroundHuman infections with the H7N9 virus could lead to lung damage and even multiple organ failure, which is closely associated a high mortality rate. However, metabolic basis of such systemic alterations remains unknown.MethodsThis study included hospitalized patients (n = 4) laboratory-confirmed infection, healthy controls 9), two disease control groups comprising pneumonia 9) who received steroid treatment 10). One H7N9-infected patient underwent biopsy for histopathological...
ABSTRACTS This research aimed to explore effects of SIX1 and DACH1 on hepatocellular carcinoma (HCC) cell proliferation, apoptosis cycle. Fifty paired tissues were screened for differentially expressed genes. expressions subjected qRT-PCR western blot in tumor cells. The knockdown efficiency siRNAs transfection cDNAs validated by as well. Then colony formation assay flow cytometry applied observe cycle changes. Immunofluorescence co-localization immunoprecipitation used analyze the...
Nanosized hydroxyapatite (HA) is a promising candidate for substitute apatite in bone biomedical applications. Furthermore, due to its excellent bioactivity, nanosized strontium-substituted HA (SrHA) has aroused intensive interest. However, the size effects of these nanoparticles on cellular bioactivity should be considered. In this study, and SrHA with different dimensions crystallization were synthesized by hydrothermal methods. The phase, chemical composition analyzed X-ray diffraction...
Abstract This study aims to investigate the effects of Cyclin D1 silencing on cell cycle, proliferation, and apoptosis hepatocellular carcinoma cells (HCC). Cells were divided into blank group, negative control group (HCC transfected with shRNA), shRNA normal (human liver L‐02 cells). Expressions D1, Caspase‐3, Bcl‐2, C‐myc detected by RT‐qPCR Western blotting. Cell proliferation was Counting Kit‐8. cycle flow cytometry. Tumor xenograft in nude mice performed detect vivo tumorigenesis. HCC...
The fabrication and application of bioactive hydroxyapatite has always been a research hot spot in the fields orthopaedics. Now it is common to use calcium (Ca) salt as Ca 2+ source synthesise hydroxyapatite. And egg shell could be another promising raw material source, which not only economical but also biogenic. In this study, (ES)-hydroxyapatite was prepared by using shells via hydrothermal method. Furthermore, ES-Sr synthesized incorporation element strontium (Sr ) into...
Background . Previously, we have reported that IL-33 functioned as a protective modulator in dextran sulfate sodium- (DSS-) induced chronic colitis by suppressing Th17 cell response colon lamina propria and both regulatory B cells (Bregs) T (Tregs) mesenteric lymph nodes (MLNs) of mice with DSS-induced acute colitis. Moreover, speculated would promote the Treg or Breg responses leading to attenuation So, investigated role on Bregs Tregs MLN mice. Methods was administered intraperitoneal...