A5016919738- Enzyme function and inhibition
- Cancer, Hypoxia, and Metabolism
- Herpesvirus Infections and Treatments
- Amino Acid Enzymes and Metabolism
- Prion Diseases and Protein Misfolding
- Cytomegalovirus and herpesvirus research
- Ion Transport and Channel Regulation
- Hemoglobin structure and function
- RNA regulation and disease
- Nitric Oxide and Endothelin Effects
- Polyamine Metabolism and Applications
- SARS-CoV-2 and COVID-19 Research
- Neurological diseases and metabolism
- Animal Virus Infections Studies
- Ion channel regulation and function
- COVID-19 Clinical Research Studies
- Viral-associated cancers and disorders
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Synthesis and Catalytic Reactions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neuroscience and Neuropharmacology Research
- Virus-based gene therapy research
- Pancreatic function and diabetes
- Viral Infections and Outbreaks Research
Institute of Virology of the Slovak Academy of Sciences
2013-2023
Biomedical Research Center of the Slovak Academy of Sciences
2016-2022
Slovak Academy of Sciences
2005-2021
Centre for Advanced Material Application of the Slovak Academy of Sciences
2021
University of Liège
2011
Gembloux Agro-Bio Tech
2011
University of Florence
2011
Nagasaki University
2000-2006
Comenius University Bratislava
2004-2005
Carbonic anhydrase IX (CA IX) is a hypoxia-induced cell surface enzyme expressed in solid tumors, and functionally involved acidification of extracellular pH destabilization intercellular contacts. Since both acidosis reduced adhesion facilitate invasion metastasis, we investigated the role CA migration, which promotes metastatic cascade. As demonstrated here, ectopically increases scattering, wound healing transwell migration MDCK cells, while an inactive variant lacking catalytic domain...
In the hypoxic regions of a tumor, carbonic anhydrase IX (CA IX) is an important transmembrane component pH regulatory machinery that participates in bicarbonate transport. Because tumor has implications for growth, invasion, and therapy, determining basis contributions CA to microenvironment could lead new fundamental practical insights. Here, we report Thr443 phosphorylation at intracellular domain by protein kinase A (PKA) critical its activation cells, with fullest activity also...
Carbonic anhydrase IX is a hypoxia-induced transmembrane enzyme linked with solid tumors. It catalyzes the reversible hydration of CO2 providing bicarbonate ions for intracellular neutralization and protons extracellular acidosis, thereby supporting tumor cell survival invasiveness. CA only human isoform containing proteoglycan (PG) domain in its part. The PG appears to enhance catalytic activity mediate binding matrix. Moreover, manipulation level by siRNA or overexpression modulates...
Most solid tumors display extracellular acidosis, which only partially overlaps with hypoxia and induces distinct adaptive changes leading to aggressive phenotype. Although acidosis is mainly attributable excessive production of lactic acid, it also involves carbonic anhydrase (CA) IX-mediated conversion CO(2) an proton a bicarbonate ion transported cytoplasm. CA IX pre-dominantly expressed in poor prognosis its transcription activity are induced by hypoxia. Here we investigated whether low...
CA IX is a hypoxia-induced, cancer-associated carbonic anhydrase isoform with functional involvement in pH control and cell adhesion. Here we describe an alternative splicing variant of the CA9 mRNA, which does not contain exons 8–9 expressed tumour cells independently hypoxia. It also detectable normal tissues absence full-length transcript can therefore produce false-positive data prognostic studies based on detection hypoxia- cancer-related expression. The encodes truncated protein...
Tumor metastasis is tightly linked with invasive membrane protrusions, invadopodia, formed by actively invading tumor cells. Hypoxia and pH modulation play a role in the invadopodia formation their matrix degradation ability. Tumor-associated carbonic anhydrase IX (CAIX), induced hypoxia, essential for regulation migration, predisposing it as an active component of invadopodia. To investigate this assumption, we employed silencing inhibition CA9, isolation assay. Quail chorioallantoic...
Adaptation to tumor hypoxia is mediated in large part by changes protein expression. These are driven multiple pathways, including activation of the inducible factor-1 (HIF-1) transcription factor and PKR-like endoplasmic reticulum kinase PERK, a component unfolded response. Through gene expression profiling we discovered that induction HIF-1 target CA9 was defective mouse embryo fibroblasts derived from mice harboring an eIF2alpha S51A knock-in mutation. This finding confirmed two isogenic...
The complete coding sequence of the herpesvirus turkeys (HVT) unique long (U L ) region along with internal repeat regions has been determined. This allows completion HVT nucleotide by linkage to short S region. genome is approximately 160 kbp and shows extensive similarity in organization genomes Marek’s disease virus serotypes 1 2 (MDV-1, MDV-2) other alphaherpesviruses. contains 75 ORFs, three ORFs present two copies. Sixty-seven were identified readily as homologues alphaherpesvirus...
Endosialin is a transmembrane glycoprotein selectively expressed in blood vessels and stromal fibroblasts of various human tumours. It has been functionally implicated angiogenesis, but the factors that control its expression have remained unclear. As insufficient delivery oxygen driving force angiogenesis growing tumours, we investigated whether hypoxia regulates endosialin expression. Here, demonstrate gene transcription induced by predominantly through mechanism involving...
// Sona Hudecova 1 , Jana Markova Veronika Simko 2 Lucia Csaderova Tibor Stracina 3 Marta Sirova Michaela Fojtu Eliska Svastova Paulina Gronesova 4 Michal Pastorek Marie Novakova Dana Cholujova Juraj Kopacek Silvia Pastorekova Jan Sedlak Olga Krizanova Institute of Clinical and Translational Research, Biomedical Research Center, SAS, Bratislava, Slovakia Virology, Department Physiology, Faculty Medicine, Masaryk University, Brno, Czech Republic Cancer Institute, Correspondence to: Krizanova,...
Tumor hypoxia represents a severe microenvironmental stress that is frequently associated with acidosis. Cancer cells respond to these stresses changes in gene expression promote survival at least part through pH regulation and metabolic reprogramming. Hypoxia-induced carbonic anhydrase IX (CA IX) plays critical adaptive role response hypoxic acidic environments by catalytically hydrating extracellular CO2 produce bicarbonate for buffering intracellular (pHi). We used proteome-wide profiling...
ABSTRACT In an attempt to identify the molecules involved in pathogenesis of prion diseases, we performed cDNA subtraction on brain tissues mice affected with experimental disease and unaffected control. The genes identified as being upregulated prion-affected tissue included those encoding a series lysosomal hydrolases (lysozyme M both isoforms β- N -acetylhexosaminidase), perforin-like protein (macrophage proliferation-specific gene-1 [MPS-1]), oxygen radical scavenger (peroxiredoxin)....
MINT‐7293982: E‐cadherin (uniprotkb:Q95LE0) and CA IX (genbank_protein_gi:223556027) colocalize (MI:0403) by fluorescence microscopy (MI:0416)
A hydrophobic, fibrillogenic peptide fragment of human prion protein (PrP 106–126) had in vitro toxicity to neurons expressing cellular (PrPC). In this study, we proved that primary cultures mouse cerebral endothelial cells (MCEC) express PrPC. Incubation MCEC with PrP 106–126 (25–200 μM) caused a dose-dependent assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase release, bis-benzimide staining for nuclear morphology, and trypan blue...