A5026694160- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Process Optimization and Integration
- Biochemical Acid Research Studies
- Vibrio bacteria research studies
- Catalysis for Biomass Conversion
Tsinghua University
2020-2025
Massachusetts Institute of Technology
2025
1,5-Pentanediol (1,5-PDO) is an important C5 building block for the synthesis of different value-added polyurethanes and polyesters. However, no natural metabolic pathway exists biosynthesis 1,5-PDO. Herein we designed constructed a promising nonnatural de novo production 1,5-PDO from cheap carbohydrates. This route expands lysine pathways employs two artificial modules to sequentially convert into 5-hydroxyvalerate (5-HV) via 5-hydroxyvaleryl-CoA. Theoretically, 5-hydroxyvaleryl-CoA-based...
Effective employment of renewable carbon sources is highly demanded to develop sustainable biobased manufacturing. Here, we developed Escherichia coli strains produce 2,3-butanediol and acetoin (collectively referred as diols) using acetate the sole source by stepwise metabolic engineering. When tested in fed-batch experiments, strain overexpressing entire utilization pathway was found consume at a 15% faster rate (0.78 ± 0.05 g/g/h) 35% higher diol titer (1.16 0.01 g/L) than baseline...
1,3-Propanediol (1,3-PDO) is a promising platform chemical used to manufacture various polyesters, polyethers, and polyurethanes. Microbial production of 1,3-PDO using non-natural producers often requires adding expensive cofactors such as vitamin B12, which increases the whole cost. In this study, we proposed engineered synthetic pathway derived from acetyl-CoA, enabling efficient accumulation in Escherichia coli without cofactors. This functional was established by introducing...
1,3-Butanediol (1,3-BDO) is an important C4 platform chemical widely used as a solvent in cosmetics and key intermediate for the synthesis of fragrances, pheromones, pharmaceuticals. The development sustainable bioprocesses to produce enantiopure 1,3-BDO from renewable bioresources by fermentation promising alternative conventional routes has aroused great interest recent years. Although two metabolic pathways have been previously established biosynthesis (R)-1,3-PDO, reported titer yield...
γ-Hydroxybutyrate (GHB) is an important C4 platform chemical, serving as a crucial precursor for the synthesis of various bulk chemicals, including γ-butyrolactone (GBL) and 1,4-butanediol (1,4-BDO). In this study, we report systematic metabolic engineering