Background Protein kinases are proven targets for drug development with an increasing number of eukaryotic Kinase (ePK) inhibitors now approved as drugs. Mitogen-activated protein kinase (MAPK) family members connect cell-surface receptors to regulatory within cells and influence a tissue-specific biological activities such cell proliferation, differentiation survival. However, the contributions MAPK pathway schistosome survival unclear. Methodology/Principal Findings We employed RNA...

Eukaryotic protein kinases (ePKs) are good medical targets for drug development in different biological systems. ePKs participate many cellular processes, including the p38 MAPK regulation of homeostasis upon oxidative stress. We propose to assess role Smp38 signaling pathway S. mansoni and protection against stress, parasite survival, also elucidate which target genes have their expression regulated by MAPK. After a significant reduction up 84% transcription level gene knockdown, no visible...

Host metabolic changes have been observed to affect Schistosoma mansoni egg production, worm survival and morphology. We examined worms recovered from streptozotocin-induced diabetic mice by morphometric morphological analysis through brightfield confocal laser scanning microscopy. Tegument thickness was slightly smaller in the reproductive organs were 23–30% consecutively 88–100% of worms. The testicular lobes had a large diminution cells one or more lobes, which associated with lack...

A computer software for image analysis (IMAGE PRO PLUS, MEDIA CYBERNETICS) was utilized in male and females adult worms, aiming the morphological characterization of Schistosoma mansoni samples isolated from a slyvatic rodent, Nectomys squamipes, humans Sumidouro, Rio de Janeiro, Brazil recovered Mus musculus C3H/He. The following characters males's testicular lobes were analyzed: number, area, density, larger smaller diameter, longer shorter axis perimeter extension; females: axis, diameter...

Aiming to detail data obtained through brightfield microscopy (BM) on reproductive, excretory and digestive system, specimens of Schistosoma mansoni eight weeks old, were recovered from SW mice, stained with Langeron's carmine analyzed under a confocal laser scanning microscope CLSM 410 (Carl Zeiss). The reproductive system presented single lobate testis, intercommunications between the lobes without efferent duct. Supernumerary testicular lobe was amorphous isolated normal ones. Collecting...

In order to evaluate the permissiveness of Nectomys squamipes Schistosoma mansoni and influence albino mice on morphological aspects adult worms derived from a population isolated N. squamipes, morphology S. Sambon, 1907 male was studied using digital image analyser (MOP VIDEOPLAN) light microscopy. Their sources were as follows: (1) recovered wild rodent Brants naturally infected Sumidouro, RJ, Brazil; (2) experimentally with strain squamipes; (3) after isolation aboriginal human infections...

Echinococcus granulosus (sensu lato) (s.l.) and vogeli are causative agents of chronic zoonotic diseases such as cystic polycystic echinococcosis, respectively. In Brazil, echinococcosis has a restricted geographical distribution in the North Region, while is observed South Region. Domestic dogs (Canis familiaris) fed with raw viscera represent risk factor for E. infection Although this practice frequent, it remains unclear whether domestic infected state Acre, located Amazon basin Region...

Abstract Background There are more than 300 species of capillariids that parasitize various vertebrate groups worldwide. Species identification is hindered because the few taxonomically informative structures available, making task laborious and genus definition controversial. Thus, its taxonomy one most complex among Nematoda. Eggs parasitic viewed in coprological analysis both modern ancient samples; consequently, their presence indicative positive diagnosis for infection. The structure...

A prevalência das enteroparasitoses foi verificada através de inquérito coproparasitológico realizado março 1990 a outubro 1991, em 1.381 pré-escolares provenientes quatro comunidades faveladas da cidade do Rio Janeiro, Brasil. Por meio técnica Blagg et al. (1955), encontrada uma positividade geral 54,5. Os parasitos mais freqüentes foram Giardia lamblia e Ascaris lumbricoides, cerca 25,0% crianças investigadas. Correlação estatisticamente significativa observada entre idade nas menores três...

A comparative morphometric study was performed to identify host-induced morphological alterations in Schistosoma mansoni adult worms. wild parasite population obtained from a naturally infected rodent (Nectomys squamipes)and then recovered laboratory C3H/He mice. Furthermore, allopatric worm populations maintained for long-term under conditions Swiss Webster mice were passed on N. squamipes. Suckers and genital system (testicular lobes, uterine egg, egg spine) analyzed by digital image...

High-fat diets induce weight gain and fatty liver in wild-type mice. Schistosomiasis mansoni infection also promotes hepatic injury. This study was designed to quantify alterations schistosomiasis mansoni-infected mice fed a high fat-rich chow compared standard rodent chow, using stereology. Female SW each either high-fat diet (29% lipids) or (12% over 8 months, then were infected with Schistosoma cercariae. Four experimental groups studied: (IHFC) (ISC), uninfected (HFC) (SC). Mice...

Malnutrition hampers the course of schistosomiasis mansoni infection just as normal growth adult worms. A comparative morphometric study on specimens (male and female) recovered from undernourished (fed with a low protein diet - regional basic diet) nourished (rodent commercial laboratory food, NUVILAB) white mice was performed. Tomographic images analysis oral ventral suckers, reproductive system tegument were obtained by means confocal laser scanning microscopy. Undernourished male...

Experiments were carried out to analyze the biological characteristics of two sympatric isolates Schistosoma mansoni derived from humans and murines in a low endemic transmission area (Sumidouro county, state Rio de Janeiro, Brazil). Sympatric reared-laboratory Biomphalaria glabrata C3H/He mice used as experimental hosts. Parameters assessed comprised: precercarial period, infectivity mortality (snails), prepatent (percentage cercariae maturation into adult worm) intestinal egg count (mice)....

A fim de verificar diferenças biológicas e morfológicas entre cepas brasileiras (CMO, CM BE) Schistosoma mansoni foram estudados os seguintes parâmetros: período pré-patente, cinética eliminação ovos nas fezes, contagem no intestino, infectividade as características fenotípicas dos vermes adultos. O pré-patente foi 42 a 44 dias. recuperação adultos variou 26% 29%, sem significativas (p>0,05) nestes parâmetros. Todas apresentaram maior quantidade intestino delgado do que grosso...

Histopathologic and morphometric (area, perimeter, major minor diameters) analysis of hepatic granulomas isolated from twelve naturally infected Nectomys squamipes were compared to four experimentally ones six C3H/He mice. Liver paraffin sections stained for cells extracellular matrix. Both groups N. presented peculiar consisting predominantly large macrophages, full schistosome pigment, characterizing an exudative-macrophage granuloma type, smaller than the equivalent type in mouse....

The morphological aspects of Schistosoma mansoni adult worms recovered from albino mice fed on a cholesterol-rich diet compared to standard chow were investigated. After feeding their respective diets for over period 5 months, subcutaneously infected with c. 50 S. cercariae/mouse. Blood samples obtained 1 day prior experimental infections and 63 days later, when euthanized by jugular section (hypovolaemic shock). Total cholesterol (TC) levels determined. Recovered stained hydrochloric...

This study investigated whether a long-term high-fat diet has an effect on the outcome of chronic murine schistosomiasis mansoni compared to standard diet. Swiss Webster female mice (3 weeks old) were fed each for up six months and then infected with 50 Schistosoma cercariae. Their nutritional status was assessed by monitoring total serum cholesterol body mass. Infected examined 6-17 post infection estimate number eggs in faeces. Mice euthanised next day. Total lower comparison uninfected...

Abstract The helminth fauna of Agouti paca (Linnaeus, 1766) has seldom been studied. In this paper, we report an unusual mixed infection Echinococcus vogeli Rausch & Bernstein, 1972 and Calodium hepaticum (syn. Capillaria hepatica Bancroft, 1863) in free-ranging from a forested region Acre (Brazil). Gross morphological examination revealed that liver contained multiple spherical to subspherical white or translucent lesions, which were isolated frequently contiguous partially covered by...