Diet-induced obesity in C57BL/6 mice triggers common features of human metabolic syndrome (MetS). The purpose is to assess the suitability a diet-induced model for investigating non-alcoholic fatty pancreatic disease (NAFPD), liver and insulin resistance. Adult were fed either high-fat chow (HFC, 60% fat) or standard (SC, 10% during 16-week period. We evaluated both groups: hepatopancreatic injuries, islets size, alpha beta-cell immunodensities, intraperitoneal tolerance test (IPITT) oral...

Substantial evidence suggests that poor intrauterine milieu elicited by maternal nutritional disturbance may programme susceptibility in the fetus to later development of chronic diseases, such as obesity, hypertension, cardiovascular disease and diabetes. One most interesting features fetal programming is from several studies consequences not be limited first-generation offspring it can passed transgenerationally. In present study, female rats (F0) were fed either a normal-protein diet...

The aim of the present study was to evaluate effects monotherapies and combinations drugs on insulin sensitivity, adipose tissue morphology, pancreatic hepatic remodelling in C57BL/6 mice fed a very HF (high-fat) diet. Male were an (60% lipids) diet or SC (standard chow; 10% for 10 weeks, after which time following drug treatments began: HF-T (HF treated with telmisartan; 5.2 mg x kg-1 body weight day-1), HF-S sitagliptin; 1.08 g weight.day-1), HF-M metformin; 310.0 HF-TM...

Browning is characterized by the formation of beige/brite fat depots in subcutaneous white adipose tissue (sWAT). This study aimed to examine whether chronic activation PPARalpha fenofibrate could induce beige cell sWAT diet-induced obese mice. High-fat fed animals presented overweight, insulin resistance and displayed adverse remodeling. Fenofibrate significantly attenuated these parameters. Treated groups demonstrated active UCP-1 clusters within sWAT, confirmed through higher gene...

Semaglutide (GLP-1 agonist) was approved for treating obesity. Although the effects on weight loss and metabolism are known, responses of adipocytes to semaglutide yet limited. C57BL/6 male mice (n = 20/group) were fed a control diet (C) or high-fat (HF) 16 weeks then separated into four groups 10/group) an additional weeks: C, C semaglutide, HF, HF semaglutide. Epididymal white adipose tissue (eWAT) subcutaneous (sWAT) fat pads studied with biochemistry, immunohistochemistry/fluorescence,...

We hypothesized that a high-fat (HF) diet aggravates ovariectomy-related complications. To test this hypothesis, ovariectomized (OVX) mice were fed HF diet, and we investigated the lipid metabolism, adipose tissue remodeling, adipokines, inflammatory cytokines. investigate situation in mouse model of ovariectomy, OVX SHAM C57BL/6 (60% fat) or standard chow (SC, 10% monitored for 18 wk. evaluated daily food intake weekly body weight. Mice killed at 30 wk age. Blood samples collected...

The chronic intake of fructose has been linked to insulin resistance, obesity, dyslipidemia and nonalcoholic fatty liver disease (NAFLD), which in turn, may progress steatohepatitis (NASH). We aimed evaluate the magnitude effects consumption high-fructose (HFr) high fat (HF) alone or combined. Four groups male mice were fed different diets for 16 weeks: standard chow (9% fat: SC), HF diet (42% fat), HFr (34% fructose) HF/HFr fat, 34% fructose). food was not among groups, body mass greater...

Background Obesity is associated with low-grade chronic inflammation attributed to dysregulated production, release of cytokines and adipokines glucose-insulin homeostasis dyslipidemia. Nutritional interventions such as dieting are often accompanied by repeated bouts weight loss regain, a phenomenon known cycling (WC). Methods In this work we studied the effects WC on feed efficiency, blood lipids, carbohydrate metabolism, adiposity inflammatory markers in C57BL/6 male mice that two or three...

Aim The aim of the present study was to evaluate whether activation peroxisome proliferator-activated receptor (PPAR)alpha and PPARgamma by Bezafibrate (BZ) could attenuate hepatic white adipose tissue (WAT) abnormalities in male offspring from diet-induced obese dams. Materials Methods C57BL/6 female mice were fed a standard chow (SC; 10% lipids) diet or high-fat (HF; 49% for 8 weeks before mating during gestation lactation periods. Male received SC at weaning subdivided into four groups:...

We studied the effect of metformin on brown adipose tissue (BAT) in a fructose-rich-fed model, focusing BAT proliferation, differentiation, and thermogenic markers.C57Bl/6 mice received isoenergetic diets for ten weeks: control (C) or high-fructose (F). For additional eight weeks, animals hydrochloride (M, 250 mg/kg/day) saline. After sacrifice, white fat pads were prepared light microscopy molecular analyses.Body mass gain, pads, adiposity index not different among groups. There was...

Here, we investigate whether a diet rich in fish oil can lead to the development of hepatic alterations associated with non-alcoholic fatty liver disease (NAFLD). To achieve this goal, provided, for 8 weeks, four different diets 3-month-old C57BL/6 mice: (a) standard-chow (SC; 40 g soybean oil/kg diet, 10 % total energy content from lipids), (b) (FO; 4 and 36 (c) high-fat (HF; 238 lard/kg 50 (d) high-fish (HFO; lipids). Biochemical analyses, stereology, western-blotting RT-qPCR were used. In...

Aim To determine the impact of paternal obesity, maternal obesity or combination two obese parents on markers adult offspring metabolism, with a focus body mass (BM), lipid and carbohydrate, components lipogenesis beta-oxidation in liver, sex dimorphism that received SC diet during postnatal period. Materials Methods Male female C57BL/6 mice were fed high-fat (HF; 49% lipids) standard chow (SC; 17% for 8 weeks before mating until lactation. The labeled according to sex, (first letters),...