A5031131396- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Resistance in Staphylococcus
- Helicobacter pylori-related gastroenterology studies
- Plant Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Genomics and Chromatin Dynamics
- Antifungal resistance and susceptibility
- Biochemical and Structural Characterization
- Infective Endocarditis Diagnosis and Management
- Advanced biosensing and bioanalysis techniques
- Pluripotent Stem Cells Research
- Nanoparticle-Based Drug Delivery
- Gut microbiota and health
- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- Click Chemistry and Applications
- Nanoplatforms for cancer theranostics
- Polyomavirus and related diseases
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Biochemical and Molecular Research
- Animal Genetics and Reproduction
- Nosocomial Infections in ICU
- Bacterial biofilms and quorum sensing
- Actinomycetales infections and treatment
Stanford University
2021-2025
Mayo Clinic Health System
2022
Emory University
2015-2021
University of North Dakota
2012-2014
Staphylococcus aureus (S. aureus) is a major human pathogen that responsible for wide range of systemic infections. Since its propensity to form biofilms in vivo poses formidable challenges both detection and treatment, tools can be used specifically image S. are highly valuable clinical management. Here, we describe the development oxadiazolone-based activity-based probes target aureus-specific serine hydrolase FphE. Because this enzyme lacks homologues other bacteria, it an ideal selective...
Staphylococcus aureus (S. aureus) is an opportunistic human pathogen that causes over one million deaths around the world each year. We recently identified a family of serine hydrolases termed fluorophosphonate binding (Fphs) play important roles in lipid metabolism and colonization host. Because many these enzymes are only expressed bacteria, they valuable targets for diagnostics therapeutics. Here, we developed screened highly diverse cyclic peptide libraries using mRNA display with...
Summary Clostridium (Clostridioides) difficile is a gastrointestinal pathogen that colonizes the intestinal tract of mammals and can cause severe diarrheal disease. Although C. growth confined to tract, our understanding specific metabolites host factors are important for bacterium limited. In other enteric pathogens, membrane‐derived metabolite, ethanolamine (EA), utilized as nutrient source function signal initiate production virulence factors. this study, we investigated effects role...
The formation of spores is critical for the survival Clostridium difficile outside host gastrointestinal tract. Persistence C. greatly contributes to spread infection (CDI), and resistance antimicrobials facilitates relapse infection. Despite importance sporulation pathogenesis, molecular mechanisms controlling spore are not well understood. initiation known be regulated through activation conserved transcription factor Spo0A. Multiple regulators influence Spo0A in other species; however,...
Clostridium difficile (also known as Peptoclostridium difficile) is a major nosocomial pathogen and leading cause of antibiotic-associated diarrhea throughout the world. Colonization intestinal tract necessary for C. to disease. Host-produced antimicrobial proteins (AMPs), such lysozyme, are present in can deter colonization by many bacterial pathogens, yet able survive colon presence these AMPs. Our prior studies established that Dlt pathway, which increases surface charge bacterium...
Accurate diagnosis of acute severe respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is critical for appropriate management patients with this disease. We examined the possible complementary role laboratory-developed class-specific clinical serology in assessing SARS-CoV-2 hospitalized patients.
To cause disease, Clostridioides (Clostridium) difficile must resist killing by innate immune effectors in the intestine, including host antimicrobial peptide, cathelicidin (LL-37). The mechanisms that enable C. to adapt intestine presence of peptides are unknown. Expression analyses revealed an operon, CD630_16170-CD630_16190 (clnRAB), which is highly induced LL-37 and not expressed response other cell-surface active antimicrobials. This operon encodes a predicted transcriptional regulator...
Clostridioides difficile causes severe antibiotic-associated diarrhea and colitis. C. is an anaerobic, Gram-positive sporeformer that highly resistant to β-lactams, the most commonly prescribed antibiotics. The resistance of β-lactam antibiotics allows pathogen replicate cause disease in antibiotic-treated patients. However, mechanisms are not fully understood.
In eukaryotes, epigenetic information can be encoded in parental cells through modification of histones and subsequently passed on to daughter a process known as transgenerational regulation. Simian Virus 40 (SV40) is well-characterized virus whose small circular DNA genome organized into chromatin and, consequence, undergoes many the same biological processes observed cellular chromatin. order determine whether SV40 capable regulation, we have analyzed from minichromosomes virions for...
We have recently shown that T-antigen binding to Site I results in the replication-dependent introduction of H3K9me1 into SV40 chromatin late infection. Since H3K9me2 and H3K9me3 are also present infection, we determined whether their presence was related status ongoing transcription replication. Transcription either inhibited with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidizole (DRB) or stimulated sodium butyrate effects on histone modifications early infection determined. The role DNA...
ABSTRACT Staphylococcus aureus ( S. ) is an opportunistic human pathogen that causes over one million deaths around the world each year. We recently identified a family of serine hydrolases termed fluorophosphonate binding (Fphs) play important roles in lipid metabolism and colonization host. Because many these enzymes are only expressed bacteria, they valuable targets for diagnostics therapeutics. Here we developed screened highly diverse cyclic peptide libraries using mRNA display with...
A wide range of synthetic polymers have been explored for antimicrobial activity. These materials usually contain both cationic and hydrophobic subunits because these two characteristics are prominent among host-defense peptides. Here, we describe a series nylon-3 containing only their evaluation against the gastrointestinal, spore-forming pathogen Clostridioides difficile. Despite highly hydrophilic nature, homopolymers showed efficacy vegetative spore forms bacterium, including an impact...
ABSTRACT Fluorescence-based contrast agents enable real-time detection of solid tumors and their neovasculature, making them ideal for use in image-guided surgery. Several have entered late-stage clinical trials or secured FDA approval, suggesting they are likely to become standard care cancer surgeries. One the key parameters optimize agent is molecular size, which dictates much pharmacokinetic pharmacodynamic properties agent. Here, we describe development a class protease-activated...
To determine facility-level factors associated with COVID-19 outbreaks in US Immigration and Customs Enforcement (ICE) detention centers.
Fluorescence-based contrast agents enable real-time detection of solid tumors and their neovasculature, making them ideal for use in image-guided surgery. Several have entered late-stage clinical trials or secured FDA approval, suggesting they are likely to become the standard care cancer surgeries. One key parameters optimize is molecular size, which dictates much pharmacokinetic pharmacodynamic properties agent. Here, we describe development a class protease-activated quenched fluorescent...
<title>Abstract</title> <italic>Staphylococcus aureus</italic> is a leading cause of bacteria-associated mortality worldwide. This largely because infection sites are often difficult to localize and the bacteria forms biofilms which not effectively cleared using classical antibiotics. Therefore, there need for new tools both image treat <italic>S. infections. We previously identified group serine hydrolases known as fluorophosphonate-binding (Fphs), regulate aspects virulence lipid...