A5060691203- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Genetic Syndromes and Imprinting
- Epigenetics and DNA Methylation
- Genetic Associations and Epidemiology
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Machine Learning in Bioinformatics
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Cell Image Analysis Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Anesthesia and Neurotoxicity Research
- Terahertz technology and applications
- Enzyme Structure and Function
- Lipid metabolism and disorders
- Restless Legs Syndrome Research
- vaccines and immunoinformatics approaches
- Advanced biosensing and bioanalysis techniques
- Protein Structure and Dynamics
Fudan University
2015-2024
State Key Laboratory of Genetic Engineering
2019-2024
Shanghai Institute of Nutrition and Health
2018-2024
University of Chinese Academy of Sciences
2021-2024
Chinese Academy of Sciences
2018-2020
Shanghai Institutes for Biological Sciences
2018-2019
Second Affiliated Hospital of Fujian Medical University
2012-2016
Fujian Medical University
2012-2016
Abstract As the largest ethnic group in world, Han Chinese population is nonetheless underrepresented global efforts to catalogue genomic variability of natural populations. Here, we developed PGG.Han, a genome database serve as central repository for data Genome Initiative (Phase I). In its current version, PGG.Han archives whole-genome sequences or high-density genome-wide single-nucleotide variants (SNVs) 114 783 individuals (a.k.a. Han100K), representing geographical sub-populations...
Single-particle cryo-electron microscopy (cryo-EM) has become a powerful technique for determining 3D structures of biological macromolecules at near-atomic resolution. However, this approach requires picking huge numbers macromolecular particle images from thousands low-contrast, high-noisy electron micrographs. Although machine-learning methods were developed to get rid bottleneck, it still lacks universal that could automatically the noisy cryo-EM particles various macromolecules.Here, we...
Abstract Multivalent drugs targeting homo-oligomeric viral surface proteins, such as the SARS-CoV-2 trimeric spike (S) protein, have potential to elicit more potent and broad-spectrum therapeutic responses than monovalent by synergistically engaging multiple binding sites on targets. However, rational design engineering of nanoscale multivalent protein are still lacking. Here, we developed a computational approach engineer self-assembling trivalent microproteins that simultaneously bind...
Acute ischemic stroke induces systemic inflammation, exhibited as changes in body temperature, white blood cell counts and C-reactive protein (CRP) levels. The aim of the present study was to observe effects intravenous thrombolytic therapy on inflammatory indices order investigate hypothesis that post-stroke response occurs necrosis brain tissues. In this study, 62 patients with acute cerebral infarction indications for thrombolysis were divided into three groups basis their treatment...
Identifying protein functional sites (PFSs) and, particularly, the physicochemical interactions at these is critical to understanding functions and biochemical reactions involved. Several knowledge-based methods have been developed for prediction of PFSs; however, accurate predicting associated with PFSs are still lacking. In this paper, we present a sequence-based method PFSs. The based on site interaction-annotated domain profile database, called fiDPD, which was built using domains found...
Abstract Comprehensive analyses of viral genomes can provide a global picture on SARS-CoV-2 transmission and help to predict the oncoming trends pandemic. This molecular tracing is mainly conducted through extensive phylogenetic network analyses. However, rapid accumulation presents an unprecedented data size complexity that has exceeded capacity existing methods in constructing evolution virus genotyping. Here we report Viral genome Evolution Network Analysis System (VENAS), which uses...
Staphylococcus aureus Cas9 (SaCas9) is a widely used genome editing tool. Understanding its molecular mechanisms of DNA cleavage could effectively guide the engineering optimization this system. Here, we determined first cryo-electron microscopy structure SaCas9-sgRNA-DNA ternary complex. This reveals that HNH nuclease domain tightly bound to site target strand, and in close contact with WED REC domains. Moreover, it captures complete sgRNA, including previously unresolved stem-loop 2. Based...
RNA-seq is one of the most widely used methods to probe gene expression and alternative splicing events (ASE) at transcriptome scale. It often generates large amounts complex sequencing data, which expands rapidly in scale studies containing many samples. Quantifying short RNA reads distribution on reference genomes a key step for analyses RNA-seq. This process requires amount computational resource, including high-end computing hardware. With accumulation high throughput results from modern...
To investigate the effects of prenatal stress on astrocytes after ischemia/reperfusion cerebral middle artery in adult offspring rats.Pregnant rats were randomly assigned to treatment group, which was exposed restraint three times daily last week pregnancy, and no group. Adult male subjected transient focal ischemia by occlusion (MCAO). There groups:prenatal stress+sham MCAO group stress+MCAO (n=10). After 5 days reperfusion, infarct size evaluated. The morphology astrocytes,...
Objective To investigate the clinical features of patients with multiple sclerosis (MS) and restless legs syndrome (RLS) to further examine relevant factors that may contribute co-occurrence MS RLS.Methods Seventy were recruited in present study.The RLS screen was performed based on diagnostic criteria for RLS.MS designated as case group without served control group.The data including age onset,MS duration disability by expanded status scale (EDSS) analyzed.Results There 12 total 70...
Abstract Influenza A virus, the H9N2 subtype, is an avian influenza virus that has long been circulating in worldwide poultry industry and occasionally found to be transmissible humans. Evidence from genomic analysis suggests provides genes for H5N1 H7N9 subtypes, which have infect mammals pose a threat human health. However, due lack of structural model interaction between host cells, mechanism extensive adaptability strong transformation capacity not fully understood. In this paper, we...
Abstract Identification and characterization of methylation quantitative trait loci (mQTLs) can help elucidate the role DNA changes as a potential mediator genetic risk loci. However, mQTLs remain poorly characterized: they have not yet been mapped in largest ethnic populations, their cell-type specific nature has resolved, proportion attributed to different molecular mechanisms is unknown. Here we perform first mQTL-mapping study large Han Chinese population, demonstrating that over 80% are...