A5060696378- Mosquito-borne diseases and control
- Malaria Research and Control
- Trypanosoma species research and implications
- Dengue and Mosquito Control Research
- Research on Leishmaniasis Studies
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Mycobacterium research and diagnosis
- Helminth infection and control
- Tuberculosis Research and Epidemiology
- Insect Resistance and Genetics
- Oil Palm Production and Sustainability
- Viral Infections and Vectors
- Blood Coagulation and Thrombosis Mechanisms
- Neuroendocrine Tumor Research Advances
- Insect Pest Control Strategies
- Lung Cancer Research Studies
- Bird parasitology and diseases
- Vector-borne infectious diseases
- Hemoglobinopathies and Related Disorders
- Peptidase Inhibition and Analysis
- Antibiotic Resistance in Bacteria
- Parasites and Host Interactions
London School of Hygiene & Tropical Medicine
2014-2024
University of London
2014-2024
Oxford Nanopore Technologies (United Kingdom)
2024
Fundação Oswaldo Cruz
2010-2015
Instituto Nacional del Tórax
2007
Benznidazole is the main drug used to treat Trypanosoma cruzi infections. However, frequent instances of treatment failure have been reported. To better understand potential resistance mechanisms, we analysed three clones isolated from a single parasite population that had undergone benznidazole-selection. These exhibited differing levels benznidazole-resistance (varying between 9 and 26-fold), displayed cross-resistance nifurtimox (2 4-fold). Each clone acquired stop-codon-generating...
Zika virus infections can cause a range of neurologic disorders including congenital microcephaly. However, while have been notified across all regions in Brazil, there has an unusual number microcephaly case notifications concentrated the Northeast country. To address this observation, we investigated epidemiological data (2014–2016) on arbovirus co-distribution, environmental and socio-economic factors for each region Brazil. Data reported cases were collected from several Brazilian...
Despite the high burden of Plasmodium vivax malaria in South Asian countries, genetic diversity circulating parasite populations is not well described. Determinants antimalarial drug susceptibility for P. region have been characterised. Our genomic analysis global (n = 558) establishes isolates 92) as a distinct subpopulation, which shares ancestry with some East African and parasites. Signals positive selection are linked to resistance-associated loci including pvkelch10, pvmrp1, pvdhfr...
Brazil is a unique and understudied setting for malaria, with complex foci of transmission associated human environmental conditions. An understanding the population genomic diversity
Chagas disease is caused by the protozoan parasite Trypanosoma cruzi and affects 5-8 million people in Latin America. Although nitroheterocyclic compound benznidazole has been front-line drug for several decades, treatment failures are common. Benznidazole a pro-drug bio-activated within mitochondrial nitroreductase TcNTR-1, leading to generation of reactive metabolites that have trypanocidal activity. To better assess action resistance, we sequenced genomes T. Y strain (35.5 Mb) three...
Drug resistance in protozoan parasites has been associated with the P-glycoprotein (Pgp), an energy-dependent efflux pump that transports substances across membrane. Interestingly, genes TcPGP1 and TcPGP2 have described Trypanosoma cruzi, although function of these not fully elucidated. The main goal this work was to investigate Pgp activity expression T. cruzi lines submitted vitro induced compounds 4-N-(2-methoxy styryl)-thiosemicarbazone (2-Meotio) benznidazole (Bz) verify stability...
Although Plasmodium vivax parasites are the predominant cause of malaria outside sub-Saharan Africa, they not always prioritised by elimination programmes. P. is resilient and poses challenges through its ability to re-emerge from dormancy in human liver. With observed growing drug-resistance increasing reports life-threatening infections, new tools inform efforts needed. In order halt transmission, we need better understand dynamics movement parasites, reservoirs infection design targeted...
Abstract Surveillance of malaria vector species and the monitoring insecticide resistance are essential to inform control strategies support reduction infections disease. Genetic barcoding mosquitoes is a useful tool assist high-throughput surveillance resistance, discriminate between sibling detect presence Plasmodium infections. In this study, we combined multiplex PCR, custom designed dual indexing, Illumina next generation sequencing for high throughput single nucleotide polymorphism...
Whole genome sequencing (WGS) of Mycobacterium tuberculosis offers valuable insights for (TB) control. High throughput platforms like Illumina and Oxford Nanopore Technology (ONT) are increasingly used globally, although ONT is known higher error rates less established genomic studies. Here we present a study comparing the outputs both platforms, analysing DNA from 59 clinical isolates in highly endemic TB regions Thailand. The resulting sequence data were to profile M. pairs their lineage,...
Abstract Vector control strategies have been successful in reducing the number of malaria cases and deaths globally, but spread insecticide resistance represents a significant threat to disease control. Insecticide has reported across Anopheles (An.) vector populations, including species within An. funestus group. These mosquitoes are responsible for intense transmission sub-Saharan Africa, Democratic Republic Congo (DRC), country contributing > 12% global infections mortality events. To...
Abstract Background Aedes spp. are responsible for the transmission of many arboviruses, which contribute to rising human morbidity and mortality worldwide. The aegypti mosquito is a main vector chikungunya, dengue yellow fever infections, whose incidence have been increasing distribution expanding. This has also driven emergence Zika virus (ZIKV), first reported in Africa spread rapidly Asia more recently across Americas. During outbreak Americas, Cape Verde became African country declaring...
Aedes mosquito vectors transmit many viruses of global health concern, including dengue, chikungunya and Zika. These vector-borne viral diseases have a limited number treatment options, vaccines vary in their effectiveness. Consequently, integrated vector management is primary strategy for disease control. However, the increasing emergence spread insecticide resistance threatening efficacy control methods. Identifying mutations associated with populations important to monitor occurrence...
Abstract Mosquitoes of the genus Aedes are main vectors many viruses, e.g. dengue and Zika, which affect millions people each year for there limited treatment options. Understanding how mosquitoes tolerate high viral loads may lead to better disease control strategies. Elucidating endogenous elements (EVEs) within vector genomes give exploitable biological insights. Previous studies have reported presence a large number EVEs in genomes. Here we investigated if flavivirus conserved across...
Each year, 92 million pregnant women are at risk of contracting malaria during pregnancy, with the underestimation mortality and morbidity burden associated Plasmodium vivax. During P. vivax infection is low birth weight, maternal anaemia, premature delivery, stillbirth. In State Acre (Brazil), high transmission leaves greater having a number recurrences. The study genetic diversity association haplotypes adverse pregnancy effects great importance for control disease. Here we investigate...
Abstract Whole genome sequencing of Mycobacterium tuberculosis offers valuable insights for (TB) control. High throughput platforms like Illumina and Oxford Nanopore Technology (ONT) are increasingly used globally, although ONT is known higher error rates less established genomic studies. Here we present a study comparing the outputs both platforms, analysing DNA from 59 clinical isolates in highly endemic regions Thailand. The resulting sequence data were to profile M. their strain-type,...