Journal Article Antidiuretic Action of Chlorpropamide in Idiopathic Diabetes Insipidus Get access F. ARDUINO, ARDUINO 1Centro de e Endocrinologia Luiz Capriglione and 3rd Medical Clinic, Federal University Rio Janeiro, Moncorvo Filho Hospital (Suseme), (GB), Brazil Search for other works by this author on: Oxford Academic Google Scholar P. J. FERRAZ, FERRAZ RODRIGUES The Clinical Endocrinology & Metabolism, Volume 26, Issue 12, 1 December 1966, Pages 1325–1328,...

This study assessed the antioxidant, antimicrobial, anticancer and neuroprotective activities of kappa(k)-carrageenan isolated from red alga Hypnea musciformis (Hm-SP). The chemical spectrum k-carrageenan Hm-SP was confirmed by Fourier transform infrared (FT-IR) spectroscopy. revealed an antibacterial antifungal action against Staphylococcus aureus Candida albicans, respectively. did not promoted cytotoxic effects Human breast cancer (MCF-7) neuroblastoma (SH-SY5Y) cell-lines. However, it...

The anti-inflammatory mechanisms of the sulfated polysaccharidic fraction obtained from red marine alga Gracilaria cornea (Gc-FI) were investigated using a paw edema model induced in rats by different inflammatory agents (carrageenan, dextran, serotonin, bradykinin, compound 48/80 or L-arginine). Gc-FI at doses 3, 9 27 mg/kg, subcutaneously - s.c., significantly inhibited rat carrageenan and as confirmed myeloperoxidase Evans’ blue assessments, respectively. (9 s.c.) histamine, L-arginine....

The aim of this study was to determine the yield, chemical composition, specific rotation (SR), infrared (IR) spectroscopy and effect on bacterial growth a crude sulfated polysaccharide (SP) from red marine alga G. ornata (Go). Go-1 (25°C), Go-2 (80°C), Go-3 (80°C) were sequentially extracted yielded 9.2%. contents sulfate (5.88-10.3%) proteins (0.1-3.7%) small. values SR [µ]D20°f -19.0, -51.0, -56.5, respectively. IR spectrums showed presence galactose-4 absence 3,6-anydrogalactose-2...

This work reports the effects of a sulfated polysaccharide (SP-Sf), isolated from seaweed Solieria filiformis and characterized by Fourier transformed infrared (FT-IR), on nociception inflammation. Male Swiss mice were pretreated with SP-Sf 30 min before receiving an injection 0.8% acetic acid, 1% formalin or prior to thermal stimulus. We observed that (1, 3 9 mg/kg) significantly reduced number writhes. also second phase test did not cause significant antinociceptive effect in hot plate...

Abstract: Seaweeds have attracted special interest as good sources of sulphated polysaccharides (SP) for use in pharmaceutical industries and biotechnology. In this study, we evaluated the effects SP from red seaweed Gracilaria cornea (Gc‐TSP) nociceptive inflammatory models. mice, Gc‐TSP (3, 9 or 27 mg/kg) significantly reduced responses, measured by number writhes, at all tested doses. a formalin test, licking time both phases test dose mg/kg. hot‐plate antinociceptive effect was observed...

Researchers see algae as a promising tool to discover both efficient and safe agents for pain therapy. We evaluated the antinociceptive anti-inflammatory activities of lectin from marine alga Pterocladiella capillacea (PcL). PcL was purified tested in classical models nociception inflammation. Male Swiss mice received 30 min prior receiving 0.8% acetic acid (10 microl/10 g, i.p.), 1% formalin (20 microl/intraplantar) or hot plate test, were compared untreated animals pretreated with...

The search for new compounds controlling pain and inflammation, with minimal side effects has focused on marine algae. aim of this work was to investigate the effect purified lectin from green alga Caulerpa cupressoides (CcL) in classical models nociception inflammation. Male Swiss mice received i.v. CcL 30 min prior receiving 0.8% acetic acid (10 ml/kg; i.p); 1% formalin (20 microl; s.c.) or were subjected thermal stimuli. We observed that (3, 9 27 mg/kg) significantly reduced number...

We investigated structural features of polysaccharides from Ulva lactuca and their effects on the classical models nociception inflammation. Crude extract was obtained by enzymatic digestion isolated ion exchange chromatography DEAE-cellulose. The fraction with higher yield used in tests (SP-Ul). Swiss mice received SP-Ul (1, 3 or 9mg/kg; i.v.), 30min prior to injection 0.8%-acetic acid 1%-formalin a thermal stimulus. At same doses, tested Wistar rats paw edema elicited different irritants...

Seaweed lectins have been widely investigated as anti-nociceptive and anti-inflammatory agents. This study analyzed the responses of a lectin from green seaweed Caulerpa cupressoides (CcL) on zymosan-induced arthritis rat temporomandibular joint (TMJ). Rats received i.v. CcL 30 min prior to injection zymosan (2 mg/art.) or 0.9% saline into left TMJ. Mechanical hyper-nociception was measured by electronic von Frey method at baseline 4 h after injection. Animals were euthanized 6 synovial...

Abstract The aim of this study was to evaluate the influence diluent, stored volume and cryodiluent medium supplementation with sulfated polysaccharides (SP) extracted from Nile tilapia skin on P. brevis vitrified sperm. Six pools were diluted in 5% Glucose or Powder Coconut Water (ACP-104), supplemented not 0.5 mg/mL SP, submitted vitrification. Subsequently, they cryotubes two volumes (60 μL 420 μL). After 15 days, samples devitrified evaluated for kinetics, membrane integrity sperm DNA...

Arthrospira platensis produced in tilapia effluent would yield sulfated polysaccharides (ApSPs). This study analyzed ApSPs by two extraction periods (3 or 24 h) on yield, physical-chemical features and vitro antioxidant effects. Dehydrated biomass from 10‰ organic system was extracted for ApSPs, with papain, 100 mM sodium acetate buffer (pH 5)+cysteine/EDTA, both 5 mM. After filtration, precipited washed alcohol were characterized electrophoreses agarose/polyacrylamide gels using known...

Prochilodus brevis is a Brazilian rheophilic fish of local market, but its bioactive glycosaminoglycans (PbGAGs) composition unknown. This study analyzed PbGAGs from gills on yield, physical-chemical features and in vitro antioxidant effects. Tritured were incubated with papain, 100 mM sodium acetate buffer (pH 5)+cysteine/EDTA, both 5 mM, to obtain PbGAGs. After extraction, characterized by agarose/polyacrylamide gels electrophoreses using known markers stained toluidine blue or Stains-All...

The cell-wall of Botryocladia occidentalis (Rhodophyta) contains sulfated galactans (BoSGs) with anti- and procoagulant properties, but their effects on thrombin generation (TG) were fewer explored. This study evaluated BoSGs by different extraction times (3, 6, 16 or 24 h) yield, physical-chemical features in vitro cephalin-induced TG 60-fold diluted human plasma using continuous system. Yield papain from ground matter increased (p<0.05) time 5.81±0.71% (BoSGs-3) to 10.03±0.74%...

Antioxidant potential of Gracilaria domingensis sulfated polysaccharides (GdSPs) has have few explored. This study sequentially extracted and compared structural antioxidant properties GdSPs. After tissue digested with papain, in 100mM sodium acetate buffer+(pH 5)+5mM cysteine/EDTA, GdSPs were quantified analyzed the molecular features (GdSPs-1→3) by agarose/polyacrylamide gels electrophoreses vs. standard glycosaminoglycans stained toluidine blue or Stains-All; structurally infrared....

Ulva fasciata (Chlorophyta) from eutrophicated areas offers bioactives sulfated polysaccharides (UfSPs). In this study papain-extracted biomass was used to quantify and analyze structural antioxidant properties of UfSPs. Sequentially the algal residues were redigested UfSPs-1→3 characterized by agarose/polyacrylamide gels electrophoreses vs. standard glycosaminoglycans stained with toluidine blue; then infrared technique. UfSPs-1/-3 in vitro evaluated for effects DPPH, total capacity ferrous...

Taxonomic value of seaweeds sulfated polysaccharides (SPs) and antioxidant potential for genus Halymenia lack studies. SPs from Caulerpa cupressoides, C. racemosa, prolifera, Botryocladia occidentalis, Gracilaria birdiae, H. pseudofloresia Solieria filiformis were analyzed their yields structural features; as antioxidants by DPPH, total capacity ferrous ion chelating (FIC) assays against BHT, ascorbic acid EDTA, respectively. Papain extraction yielded (p < 0.001) 0.42 ± 0.08% (C....

Complex cell wall composition of Lobophora variegata (Ochrophyta) rich in sulfated polysaccharides (LvSPs) is few understood. In this study, LvSPs were sequentially extracted and compared on their yield structural properties. Decumbent morphotype was digested with papain (3 or 24 h) then LvSPs3h-1→4 LvSPs24h-1→6 quantified examined charge/mass by electrophoreses (agarose/polyacrylamide gels) vs. standard glycosaminoglycans stained toluidine blue Stains-All. The features analyzed infrared...

Objetivo: Avaliar o efeito da salinidade no cultivo microalga Chaetoceros neogracilis para produção de pigmentos fotossintetizantes e exopolissacarídeos. Método: Foram realizados sete tratamentos com diferentes salinidades, por dias. Diariamente foram realizadas contagens celular medição absorbância, analisar crescimento a densidade celular. Os fotossintéticos determinados espectrofotometria, os exopolissacarídeos precipitação alcoólica do sobrenadante. Resultados: Na 25‰ ocorreu maior valor...

Red algae sulfated polysaccharides (SPs) have been widely described as anticoagulant and antithrombotic agents; however no description of activity regarding green SPs has reported. Caulerpa cupressoides (Chlorophyta) three different fractions (SP1, SP2 SP3). We investigated the effects on thrombin by antithrombin in an experimental model venous thrombosis rats. The inhibition assay was evaluated using (AT) presence rats with thromboplastin thrombogenic stimulus. are suggested be due to...

The aim of this study was to evaluate certain molecular characteristics a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed ion-exchange chromatography on DEAE-cellulose column. fractions obtained analyzed for mass, 0.5% agarose gel electrophoresis and chemical composition. activated partial thromboplastin time (APTT) test applied using normal human plasma...

Contents Poor sperm viability post‐thaw has resulted in constant research into methods of cryopreservation canine semen. One factor that may be involved poor is oxidative stress caused by excessive formation reactive oxygen species. The present study was performed order to evaluate the effect different concentrations ascorbic acid (AA) and glutathione (Glu) added an extender for freeze‐thawing dog sperm. Semen from five mature dogs collected frozen two studies. Prior after freezing,...