A5100695662- Particle physics theoretical and experimental studies
- Quantum Chromodynamics and Particle Interactions
- High-Energy Particle Collisions Research
- Neutrino Physics Research
- Black Holes and Theoretical Physics
- Computational Physics and Python Applications
- Dark Matter and Cosmic Phenomena
- CRISPR and Genetic Engineering
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Atomic and Subatomic Physics Research
- Heat shock proteins research
- Cancer, Hypoxia, and Metabolism
- Ion channel regulation and function
- Protein Hydrolysis and Bioactive Peptides
- Particle Accelerators and Free-Electron Lasers
- Medical Imaging Techniques and Applications
- Magnetic properties of thin films
- Bacterial biofilms and quorum sensing
- Pluripotent Stem Cells Research
- Hepatitis B Virus Studies
- RNA Interference and Gene Delivery
- Connexins and lens biology
- Neuroscience and Neural Engineering
- Endoplasmic Reticulum Stress and Disease
Henan University of Science and Technology
2017-2025
Experimental Center of Forestry in North China
2019-2025
Chinese University of Hong Kong, Shenzhen
2022-2025
Zhongshan Hospital
2025
Fudan University
2021-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024-2025
Children's Hospital of Fudan University
2024
State Key Laboratory of Medical Neurobiology
2024
Fu Wai Hospital
2024
Institute of High Energy Physics
2019-2024
A rapid and sensitive method is crucial for nucleic acid detection. Recently, RNA-guided CRISPR/Cas12a nuclease-based methods present great promise In the methods, however, DNA amplification subsequent Cas12a cleavage separated whole process takes as long 2 h. Most importantly, uncapping operation increases risk of aerosol contamination. this study, we propose a CRISPR/Cas12a-based named "Cas12aVDet" By integrating recombinase polymerase (RPA) with in single reaction system, detection can be...
Compact CRISPR/Cas9 systems that can be packaged into an adeno-associated virus (AAV) hold great promise for gene therapy. Unfortunately, currently available small Cas9 nucleases either display low activity or require a long protospacer adjacent motif (PAM) sequence, limiting their extensive applications. Here, we screened panel of and identified ortholog from Staphylococcus auricularis (SauriCas9), which recognizes simple NNGG PAM, displays high genome editing, is compact enough to AAV...
The tumor microenvironment (TME) is characterized by several immunosuppressive factors, of which weak acidity and l-arginine (l-arg) deficiency are two common features. A acidic environment threatens the survival immune cells, insufficient l-arg will severely restrain effect antitumor responses, both affect efficiency cancer treatments (especially immunotherapy). Meanwhile, essential for progression. Thus, strategies, supplementation deprivation, developed treatment. However, these...
Abstract Members of the solute carrier organic anion transporting polypeptide (OATPs) family function as transporters for a large variety amphipathic anions including endogenous metabolites and clinical drugs, such bile salts, steroids, thyroid hormones, statins, antibiotics, antivirals, anticancer drugs. OATP1B1 plays vital role in substances into liver hepatic clearance. FDA EMA recommend conducting vitro testing drug–drug interactions (DDIs) involving OATP1B1. However, structure working...
Human brain tissue models and organoids are vital for studying modeling human neurological disease. However, the high cost of long-term cultured inhibits their wide-ranging application. It is therefore urgent to develop methods cryopreservation organoids. Here, we establish a method using methylcellulose, ethylene glycol, DMSO, Y27632 (termed MEDY) cortical without disrupting neural cytoarchitecture or functional activity. MEDY can be applied multiple brain-region-specific organoids,...
A large number of SpCas9 orthologs has been computationally identified, but their genome editing potential remains largely unknown. In this study, a GFP-activation assay was used to screen panel 18 orthologs, ten which demonstrated activity in human cells. Notably, these had preference for purine-rich PAM sequences. Four the tested displayed enhanced specificity compared SpCas9. Of particular interest is SeqCas9, recognizes simple NNG and displays comparable SpCas9-HF1. addition, SeqCas9...
A coarse-grained (CG) model for peptides and proteins was developed as an extension of the Surface Property fItting Coarse grAined (SPICA) force field (FF). The designed to examine membrane that are fully compatible with lipid membranes SPICA FF. preliminary version this protein created using thermodynamic properties, including surface tension density in (formerly called SDK) In study, we improved CG facilitate molecular dynamics (MD) simulations a reproduction multiple properties from both...
β/γ-Crystallins are predominant structural proteins in the cytoplasm of lens fiber cells and share a similar fold composing four Greek-key motifs divided into two domains. Numerous cataract-causing mutations have been identified various β/γ-crystallins, but mechanisms underlying cataract caused by most remains uncharacterized. The S228P mutation βB1-crystallin has linked to autosomal dominant congenital nuclear cataract. Here we found that mutant was prone aggregate degrade both human E....
The gel properties and structural changes of sweet potato starch induced by heat-moisture treatment (HMT) were investigated, its vermicelli textural revealed. HMT increased the relative crystallinity, crystallinity reduced with increasing moisture content, which was consistent results DSC. RVA revealed that starches showed an setback viscosity a breakdown viscosity, indicating lead stronger retrogradation tendency. Rheometer indicated had elasticity behavior. content amylose possible fine...
The sodium-chloride cotransporter NCC mediates the coupled import of sodium and chloride across plasma membrane, playing vital roles in kidney extracellular fluid volume blood pressure control. Here, we present full-length structure human NCC, with 2.9 Å for transmembrane domain 3.8 carboxyl-terminal domain. adopts an inward-open conformation a domain-swap dimeric assembly. Conserved ion binding sites among cation-chloride cotransporters Na2 site are observed our structure. A unique His...
Abstract The cultivation and differentiation of human embryonic stem cells (hESCs) into organoids are crucial for advancing new drug development personalized cell therapies. Despite establishing chemically defined hESC culture media over the past decade, these media's reliance on growth factors, which costly prone to degradation, poses a challenge sustained stable culture. Here, we introduce an system(E6Bs) that facilitates long-term, genetically expansion hESCs, enabling consistently...
Heavy metal/ferromagnetic metal bi-layered structures that exhibit both strong perpendicular magnetic anisotropy (PMA) and large spin-orbit torque (SOT) efficiency have high potential in high-density, low-power memory, logic device applications. Here, we report the enhancement of PMA SOT Ta/Pt/Co/Ta multi-layered heterostructures through interfacial diffusion. The can fields 9100 Oe at 300 K 14100 10 an effective spin Hall angle (SHA) 0.61 ± 0.03 K. These values are larger than corresponding...