A5100545142- Rabies epidemiology and control
- Viral Infections and Immunology Research
- Virology and Viral Diseases
- Poxvirus research and outbreaks
- Animal Disease Management and Epidemiology
- Bacillus and Francisella bacterial research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Streptococcal Infections and Treatments
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Yersinia bacterium, plague, ectoparasites research
- Viral Infections and Vectors
- Immunotherapy and Immune Responses
- Insect Resistance and Genetics
- Vector-borne infectious diseases
- Viral Infections and Outbreaks Research
- Cytomegalovirus and herpesvirus research
- Respiratory viral infections research
- RNA Research and Splicing
- Venomous Animal Envenomation and Studies
Wuhan Institute of Bioengineering
2015-2025
Zhoukou Normal University
2024
National Vaccine and Serum Institute
2019
Coxsackievirus A6 (CV-A6) and A10 (CV-A10) have been emerging as the prevailing serotypes overtaking Enterovirus A71 (EV-A71) A16 (CV-A16) in most areas main pathogens of hand, foot mouth disease (HFMD) China since 2013. To investigate whole etiological spectrum following EV-A71 vaccination approximate 40,000 infants young children Xiangyang, enteroviruses were serotyped 4415 HFMD cases from October 2016 to December 2017 using Real Time conventional PCR cell cultures. Of typeable 3201...
The baculovirus insect cell expression system is pivotal for exogenous protein expression. However, serial passages of in cells often result defective virus generation and a rapid decline expression, limiting its wider application. Previous studies have shown that the late factor 5 (lef5) from other baculoviruses can enhance stability genes Autographa californica multiple nucleopolyhedrovirus (AcMNPV). In this study, we engineered diverse recombinant strains AcMNPV to express enhanced green...
To investigate the evolutionary dynamics of rabies virus (RABV) in China, we collected and sequenced 55 isolates sampled from 14 Chinese provinces over last 40 years performed a coalescent-based analysis G gene. This revealed that RABV currently circulating China is composed three main groups. Bayesian coalescent estimated date most recent common ancestor for current strains to be 1412 (with 95 % confidence interval 1006–1736). The mean substitution rate gene sequences (3.961×10−4...
Coxsackievirus A6 (CV-A6) has been emerging as a major pathogen of hand, foot and mouth disease (HFMD). Study on the pathogenesis CV-A6 infection development vaccines is hindered by lack appropriate animal models. Here, we report an actively immunized-challenged mouse model to evaluate efficacy Vero-cell-based, inactivated vaccine candidate. The neonatal Kunming mice were inoculated with purified, formaldehyde-inactivated days 3 9, followed challenging day 14 naturally selected virulent...
The vaccine candidate strain CV-A5 was produced with a high infectivity titer and viral particle yield. Three forms, empty particles (EPs), full (FPs), dense (DPs), were obtained characterized after purification.
Hand, foot and mouth disease (HFMD) is caused by a variety of serotypes in species A the Enterovirus genus, including recently re-emerged Coxsackievirus A2 (CV-A2), CV-A4 CV-A5. For development diagnostic reagents, for surveillance, multivalent vaccines against HFMD, antigenicity HFMD-associated enteroviruses warrants investigation. The purified virions were inoculated into Balb/c mice hybridomas obtained secreting monoclonal antibodies (mAbs) directed cross-reacting with other closely...
Rabies virus (RABV) causes severe neurological disease and death. As an important mechanism for generating genetic diversity in viruses, homologous recombination can lead to the emergence of novel strains with increased virulence changed host tropism. However, it is still unclear whether plays a role evolution RABV. In this study, we isolated sequenced four circulating RABV China. Phylogenetic analyses identified lineage hybrid origin that comprises two different strains, J CQ92. Analyses...
In recent years, Coxsackievirus A2 (CV-A2) has become one of the main serotypes enterovirus species A associated with hand, foot and mouth disease (HFMD) in China. It also caused HFMD epidemics many countries all over world. Currently, there are no effective, preventive vaccines against it. CV-A2 strain was isolated RD cells then adapted to grow Vero cells. This is compliance guidelines for cell substrates allowed human by Chinese regulatory authority. Groups newborn Kunming mice were...
As SARS-CoV-2 continues to evolve and COVID-19 cases rapidly increase among children adults, there is an urgent need for a safe effective vaccine that can elicit systemic mucosal humoral immunity limit the emergence of new variants. Using Chinese Hu191 measles virus (MeV-hu191) strain as backbone, we developed MeV chimeras stably expressing prefusion forms either membrane-anchored, full-length spike (rMeV-preFS), or its soluble secreted trimers with help SP-D trimerization tag (rMeV-S+SPD)...
The enterovirus A71 (EV71) inactivated vaccine is an effective intervention to control the spread of virus and prevent EV71-associated hand, foot, mouth disease (HFMD). It widely administered infants children in China. empty particles (EPs) full (FPs) generated during production have different antigenic immunogenic properties. However, antigen detection methods currently used were established without considering differences antigenicity between EPs FPs. There also a lack other analytical for...
Coxsackievirus A10 (CV-A10) has become one of the major pathogens hand, foot and mouth disease (HFMD), studies on vaccine animal model CV-A10 are still far from complete. Our study used a mouse-adapted strain, which was lethal for 14-day-old mice, to develop an infected mouse model. Then this employed establish actively immunized-challenged evaluate efficacy formaldehyde-inactivated vaccine, prepared Vero cell-adapted strain. at dose 0.5 or 2.0 μg inoculated intraperitoneally in neonatal...
A comprehensive monitoring strategy is vital for tracking the spread of mosquito-borne Japanese encephalitis virus (JEV), leading cause viral in Asia. Virus detection consists passive surveillance primarily humans and swine, and/or active mosquitoes, which may be a valuable proxy providing insights into ecological processes underlying persistence JEV. However, it has not been well characterized whether alone can capture circulating genetic diversity to make reasonable inferences. Here, we...
Rabies remains endemic in China and continues to pose a major threat public health with nearly 100 % case fatality rate humans. We confirmed of human rabies Wuhan, May 2018. The patient had got dog bite wound 3 years before symptoms confusion, hydrophobia, photophobia onset. On 14, our laboratory that the was infected virus circulates dogs died on 24, two weeks later after admission. Complete glycoprotein gene sequences determined for this isolate indicated source RABV infection dog-related variants.