A5103242273- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Bone Tissue Engineering Materials
- RNA Interference and Gene Delivery
- Electrospun Nanofibers in Biomedical Applications
- Immune cells in cancer
- Nanoplatforms for cancer theranostics
- Circular RNAs in diseases
- Diabetes Management and Research
- Hydrogels: synthesis, properties, applications
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Cardiovascular Disease and Adiposity
- Phagocytosis and Immune Regulation
- Autophagy in Disease and Therapy
- Lymphoma Diagnosis and Treatment
- Pancreatic function and diabetes
- Viral-associated cancers and disorders
- Bone Metabolism and Diseases
- Magnesium Alloys: Properties and Applications
- Dental materials and restorations
- Immunotherapy and Immune Responses
- Acute Lymphoblastic Leukemia research
- Thallium and Germanium Studies
- Chronic Myeloid Leukemia Treatments
Peking University
2021-2025
Air Force Medical University
2018-2024
Nanjing University of Information Science and Technology
2023
Beijing University of Chemical Technology
2020-2022
National Clinical Research Center for Digestive Diseases
2022
State Key Laboratory of Organic-Inorganic Composite Materials
2022
Xijing Hospital
2021
Background: Efficient and specific induction of cell death in liver cancer is urgently needed. In this study, we aimed to design an exosome-based platform deliver ferroptosis inducer (Erastin, Er) photosensitizer (Rose Bengal, RB) into tumor tissues with high specificity. Methods: Exosome donor cells (HEK293T) were transfected control or CD47-overexpressing plasmid. Exosomes isolated loaded Er RB via sonication method. Hepa1-6 xenograft C57BL/6 model was injected engineered exosomes tail...
Rationale: Exosomes are emerging as a promising drug delivery carrier.However, rapid uptake of exosomes by the mononuclear phagocyte system (MPS) remains an obstacle for into other targeted organs, including heart.We hypothesized that prior blocking MPS would improve their to organs.Methods: were isolated from cell culture medium.Fluorescence-labeled tracked in vitro and vivo fluorescence imaging.The expression clathrin heavy chain (Cltc), cavolin1, Pak1 Rhoa, known genes endocytosis,...
Abstract Background Periodontitis is characterized by progressive inflammation and alveolar bone loss resulting in tooth finally. Macrophages including pro-inflammatory M1-like macrophages reparative M2-like play a vital role tissue homeostasis periodontitis. Among them, have been shown to promote repair prevent loss. However, the mechanism of M2 macrophages-induced osteoprotective effect remains elusive. Results Exosomes from (M2-Exos) were isolated identified successfully. M2-Exos could...
Exosomes derived from mesenchymal stem cells (MSCs) have demonstrated regenerative potential for cell-free bone tissue engineering, nevertheless, certain challenges, including the confined therapeutic potency of exosomes and ineffective delivery method, are still persisted. Here, we confirmed that hypoxic precondition could induce enhanced secretion human exfoliated deciduous teeth (SHEDs) via comprehensive proteomics analysis, corresponding (H-Exo) exhibited superior in promoting cellular...
Sepsis is one of the most common causes death, which closely related to uncontrolled systemic inflammation. Dysregulation M1 macrophage polarization primary contributor serious In this study, it revealed that murine homologue circRNA SCAR (steatohepatitis-associated ATP5B regulator), denoted as mSCAR hereafter, decreases in macrophages septic mice, correlates with excessive polarization. To restore mitochondria, exosomes encapsulated are further electroporated...
A pioneering epidemic study has revealed a strong association between obesity and the risk of colitis. In this study, high fat diet was found to significantly aggravate colitis induced by dextran sulfate sodium (DSS). Meanwhile, changed miRNA profile visceral adipose exosomes, switching exosomes from anti-inflammatory pro-inflammatory phenotype. Strikingly, these inflammatory efficiently circulated into lamina propria intestine, while predisposed intestine inflammation via promoting...
Rationale:Reciprocal interactions between leukemic cells and bone marrow mesenchymal stromal (BMMSC) remodel the normal niche into a malignant niche, leading to leukemia progression.Exosomes have emerged as an essential mediator of cell-cell communication.Whether exosomes involved in remodeling remains unknown.Methods: We investigated role molecular functional changes BMMSC vitro vivo.RNA sequencing bioinformatics were employed screen for miRNAs that are selectively sorted corresponding RNA...
Abstract Therapeutic ions, such as Si and Mg, play vital roles in regulating metabolism promoting tissue repair, hence providing an efficient strategy engineering. The regenerative outcome is strongly dependent on the targeted delivery controlled release of therapeutic ions. Nevertheless, it remains challenging to deliver multiple ions at manners ratios. Herein, hierarchical ion‐based microspheres are fabricated. coordinated Mg pre‐set ratios achieved, based which, favors osteogenesis by...
Dental pulp necrosis are serious pathologic entities that causes tooth nutrition deficiency and abnormal root development, while regeneration of functional tissue is paramount importance to regain vitality. However, existing clinical treatments, which focus on replacing the necrotic with inactive filling materials, fail restore vitality functions, thus resulting in a devitalized weakened tooth. Currently, dental via stem cell-based therapy for pulpless teeth has raised enormous attention...
Prostate cancer (PCa) responds poorly to routine immunotherapy due the tumor immunosuppressive microenvironment. Here, we describe an ultrasound-based drug delivery strategy stimulate potent anti-tumor immunity via exosomes encapsulated with sonosensitizers Chlorin e6 (Ce6) and immune adjuvant R848, namely ExoCe6+R848. ExoCe6+R848 was constructed by simple co-incubation of Ce6 R848 HEK 293T cell-derived exosomes. The properties were not affected after loading accumulated in site intratumoral...
Abstract Brain metastasis (BM) is one of the leading causes cancer-related deaths in patients with advanced non-small cell lung cancer (NSCLC). However, limited treatments are available due to presence blood-brain barrier (BBB). Upregulation lysophosphatidylcholine acyltransferase 1 (LPCAT1) NSCLC has been found promote BM. Conversely, downregulating LPCAT1 significantly suppresses proliferation and cells. In this study, we firstly confirmed significant upregulation BM sites compared primary...
Abstract Current scaffolds applied for bone tissue engineering are still lacking sufficient osteogenic capacity to induce efficient regeneration. Biodegradable microsphere‐type designed achieve the dual‐controlled release of a Chinese medicine (i.e., icariin, ICA) and bioactive ion Mg 2+ ), in order their synergistic effect on inducing osteogenesis. The hydrophobic icariin is preloaded onto MgO/MgCO 3 (1:1 weight ratio) particles at different amounts then encapsulated into biodegradable...
The design of a scaffold that can regulate the sequential differentiation bone marrow mesenchymal stromal cells (BMSCs) according to endochondral ossification (ECO) mechanism is highly desirable for effective regeneration. In this study, we successfully fabricated dual-networked composite hydrogel composed gelatin and hyaluronic acid (termed GCDH-M), which sequentially release chondroitin sulfate (CS) magnesium/silicon (Mg/Si) ions provide spatiotemporal guidance chondrogenesis,...
Efficient and topical delivery of drugs is essential for maximized efficacy minimized toxicity. In this study, we aimed to design an exosome-based drug platform endowed with the ability escaping from phagocytosis at non-target organs controllably releasing targeted location.The swtichable stealth coat CP05-TK-mPEG was synthesized anchored onto exosomes through interaction between peptide CP05 exosomal surface marker CD63. Chlorin e6 (Ce6) loaded into by direct incubation. Controllable...
This study intends to improve the antibacterial and mineralization performance of photocurable dental resin composites (DRCs) reduce possibility repair failure caused by secondary caries. To end, functionalized hydroxyapatite (HAp), including Zn-doped (Zn/HAp) Sr-doped HAp (Sr/HAp), were added into bisphenol A glycidyl methacrylate triethylene glycol dimethacrylate mixture, providing DRCs with capacity, respectively. By controlling total amount inorganic filler at 70 wt%, these powders...
Exosomes are recognized as effective platforms for targeted delivery their high physicochemical stability and biocompatibility. However, most of the exosomes inevitably rapidly cleared by mononuclear phagocytic system (MPS) during cancer therapy. How to engineer exosome enhance efficiency is being intensively explored. In this study, we have constructed mPEG2000-TK-CP05 decorated achieve enhanced photodynamic/chemical therapy.Exosomes were coated with CP05-TK-mPEG2000, in which CP05 a...
Abstract Cancer‐associated cachexia (CAC) has tremendous effects on the patient's tolerance to chemotherapy and quality of life, especially in advanced stages, such as acute terminal stages chronic myeloid leukemia (CML). However, underlying mechanisms mediators remain unclear. Here, we showed that mice injected with CML‐derived exosomes had significant weight loss great drop body fat rate. In meanwhile, found could be taken up by adipose tissue, and, turn, suppressed adipogenic ability...