Abstract Fibrosis in animal models and human diseases is associated with aberrant activation of the Wnt/β‐catenin pathway. Despite extensive research efforts, effective therapies are still not available. Myofibroblasts major effectors, responsible for extracellular matrix deposition. Inhibiting proliferation myofibroblast crucial treatment fibrosis. Proliferation myofibroblasts can have many triggering effects that result In recent years, Wnt pathway has been studied as an underlying factor...

Abstract Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation. Macrophages accelerate the pathological process of RA through continuous cell accumulation and secretion cytokines. Therefore, multiscale visualization macrophages great significance for early diagnosis RA. In this work, folate receptor‐targeted Cptnc‐4F nanoprobes, named FA‐CF‐NPs, are developed dual‐model molecular imaging in mouse models. FA‐CF‐NPs exhibit complementary advantages with...

Epithelial-to-mesenchymal transition (EMT) is a crucial event in the cellular origin of myofibroblasts that secrete extracellular matrix progression pulmonary fibrosis (PF). High-mobility group box 1 (HMGB1) novel mediator EMT. However, whether this process involves recognized transforming growth factor-<i>β</i>1 (TGF-<i>β</i>1)/Smad signaling also contributes to EMT PF has not yet been elucidated. Here, we developed model induced by bleomycin (BLM) rats and conducted several simulation...

Exosome-mediated nucleic acids delivery has been emerging as a promising strategy for gene therapy. However, the intrinsic off-target effects due to non-specific uptake of exosomes by other tissues remain big hurdle clinical application. In this study, we aimed enhance efficacy and minimize simultaneously encapsulating engineered mRNA translationally activated tissue-specific microRNA (miRNA) increasing targeted efficiency via ultrasound-targeted microbubble destruction (UTMD). Briefly,...

Pulmonary fibrosis (PF) is a serious chronic lung disease with unknown pathogenesis. Researches have confirmed that oxidative stress which regulated by NADPH oxidase-4 (NOX4), main source of reactive oxygen species (ROS), an important molecular mechanism underlying PF. Previous studies showed total glucosides Danggui Buxue Tang (DBTG), extract from classical traditional Chinese herbal formula, (DBT), attenuated bleomycin-induced PF in rats. However, the mechanisms DBTG are still not clear....

Efficient and topical delivery of drugs is essential for maximized efficacy minimized toxicity. In this study, we aimed to design an exosome-based drug platform endowed with the ability escaping from phagocytosis at non-target organs controllably releasing targeted location.The swtichable stealth coat CP05-TK-mPEG was synthesized anchored onto exosomes through interaction between peptide CP05 exosomal surface marker CD63. Chlorin e6 (Ce6) loaded into by direct incubation. Controllable...

Glioblastoma (GBM) is the commonest form of primary brain tumor in central nervous system, with median survival below 15 months and only a 25% two-year rate for patients. One major clinical challenges treating GBM presence blood–brain barrier (BBB), which greatly limits availability therapeutic drugs to tumor. Ultrasound-mediated BBB opening provides promising approach help deliver tumors. The use temozolomide (TMZ) treatment has been shown be able increase patients GBM, but this improvement...

Multidrug resistance is one of the major reasons colorectal cancer (CRC) chemotherapy-based treatments fail, and novel biologically based therapies are urgently needed. Src homology 3 (SH3)-domain GRB2-like protein 1 (SH3GL1) a membrane-bound which was found to be involved in tumor formation, progression, metastasis. In this study, immunohistochemistry staining, quantitative real-time polymerase chain reaction (qRT-PCR), western blot analysis revealed high expression SH3GL1 human CRC...

The early detection of atherosclerotic lesions is particularly important for risk prediction acute cardiovascular events. Macrophages apoptosis was significantly associated with the degree AS and especially contributed to plaque vulnerability. In this research, we mainly sought explore feasibility a home-made AV-nanobubbles (NBAV) visualization apoptotic macrophages assessment atherosclerosis (AS) by contrast-enhanced ultrasound (CEUS) imaging.NBAV were prepared "Optimized Thin-Film...

Objective To investigate the safety and mid-term outcomes of hydrodissection-assisted microwave ablation (MWA) hepatocellular carcinoma (HCC) in various difficult locations. Methods A total 131 HCC patients who underwent ultrasound-guided MWA from March 2017 to 2019 were included. Following ultrasound examination, with tumors at locations treated (hydrodissection group), while those conventional received (control group). Both groups compared concerning baseline characteristics, parameters,...

West Nile virus (WNV) is an important neurotropic that accounts for emergence of human arboviral encephalitis and meningitis. The interaction WNV with signaling pathways plays a key role in controlling infection. We investigated roles AKT ERK supporting propagation modulating inflammation response following established productive infection neuronal cell lines originated from mouse. Expression IL-11 TNF-α was markedly up-regulated the infected cells, indicating elicitation upon incubation...

West Nile virus (WNV) is a rapidly spreading mosquito-borne accounted for neuroinvasive diseases. An insight into WNV-host factors interaction necessary development of therapeutic approaches against WNV infection. CD11b has key biological functions and been identified as target several human The purpose this study was to determine whether implicated in

Osteonecrosis of the femoral head (ONFH) is a disease that commonly seen in clinic, but its detection rate remains limited, especially at early stage. We developed an ultrasound molecular imaging (UMI) approach for diagnosis ONFH by detecting expression integrin αvβ3 during pathological changes steroid-induced osteonecrosis (SIONFH) rat models. The αvβ3-targeted PLGA or lipid microbubbles modified with iRGD peptides were fabricated and characterized. Their adhesion efficiency to mouse brain...

Objective To investigate the feasibility and clinical application of anterior medial fenestration approach in treatment Pipkin type I II femoral head fractures. Methods The hips two anti‐corrosion adult specimens treated with formalin were dissected anatomical structures directional characteristics main muscles, ligaments, blood vessels, nerves observed. was performed on bilateral four fresh frozen to determine required pulling direction stripped muscles ligaments during surgery. In...

Objectives To study the technical feasibility of contrast‐enhanced ultrasound (CEUS) in evaluating femoral head perfusion a rabbit model steroid‐induced osteonecrosis. Methods Twenty rabbits were divided randomly into control group (n = 8) and an experimental 12). Rabbits induced by lipopolysaccharide methylprednisolone to build Contrast‐enhanced examinations performed at 3 5 weeks after induction. Then, pathologic microvessel density (MVD) calculations on excised heads. Results The MVD...

This study aimed to evaluate the annual pollution characteristics of trichloromethane (TCM) in Kunshan City’s tap water from 2016 2022. research analyzed 566 samples centralized supply units, utilizing GB 5749-2006 Sanitary Standard for Drinking Water as evaluation benchmark. Data analysis employed non-parametric tests and Spearman’s correlation using Excel 2017 SPSS 26.0. The results indicated a 100% compliance rate with TCM limit (0.06 mg/L), median concentrations ranging 0.1 6.4 μg/L....

The agroforestry system provides a new option for P. notoginseng cultivation. However, the effects on soil health and microbial communities are still incomplete when monoculture coniferous forests converted to notoginseng–pine plantations. To assess health, samples from five plantations were collected, including healthy plants root-diseased plants, as well plantation control soil. analyzed physical, chemical, biological aspects communities. Our results suggested that planted under pine...

With the development of endoscopic technology, detection rate synchronous multiple primary early esophageal cancer (SMPEEC) is increasing; however, risk factors remain unclear. We aimed to assess clinicopathological characteristics patients with SMPEEC and investigate contributing lesions. A retrospective cohort study was conducted on 911 consecutive who underwent Endoscopic submucosal dissection (ESD) for neoplasms from January 2013 June 2021. The were divided into group solitary (SEEC)...

Objective: To investigate the predictive value of contrast-enhanced ultrasound (CEUS) combined with serum miR-124 level in acute cerebral infarction (ACI) and their association contrast enhancement carotid atherosclerotic plaque. Methods: Totally 60 patients diagnosed ACI controls were included study. All subjects had plaques, all them examined by CEUS tested for levels. Results: Time to peak (TTP) mean transit time (MTT) group significantly shorter than those control (P < 0.05), but...

The aim of this study was to quantitatively assess subchondral bone microcirculation perfusion in adults with early osteonecrosis the femoral head (ONFH) using contrast-enhanced ultrasound (CEUS) and evaluate its correlation Association Research Circulation Osseous (ARCO) stage. We investigated 97 adult patients definite ONFH by imaging a total 155 hips, performing CEUS, storing images CEUS processes at different ARCO stages generating time-intensity curves (TICs) obtain parameters....