A5013388930- Reproductive Biology and Fertility
- Assisted Reproductive Technology and Twin Pregnancy
- Prenatal Screening and Diagnostics
- Sperm and Testicular Function
- Reproductive Health and Technologies
- Ovarian function and disorders
- Pluripotent Stem Cells Research
- Renal and related cancers
- Animal Genetics and Reproduction
- Cardiac pacing and defibrillation studies
- Genomic variations and chromosomal abnormalities
- SARS-CoV-2 and COVID-19 Research
- Genetic Syndromes and Imprinting
- Reproductive System and Pregnancy
- Sexual Differentiation and Disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cardiomyopathy and Myosin Studies
- Endometriosis Research and Treatment
- 3D Printing in Biomedical Research
- Cardiac Valve Diseases and Treatments
- Plant Diversity and Evolution
- SARS-CoV-2 detection and testing
- Cardiac Structural Anomalies and Repair
- COVID-19 Clinical Research Studies
- Bipolar Disorder and Treatment
Cambridgeshire and Peterborough NHS Foundation Trust
2021
Fulbourn Hospital
2021
Barcelona Biomedical Research Park
2020-2021
National Institute of Dental and Craniofacial Research
2017
University of New Orleans
2015
Barcelona IVI Clinic
2007-2014
IVI Murcia Clinic
2013
Valencian Infertility Institute
2010-2011
Universitat Autònoma de Barcelona
1995-2010
IVI Sevilla Clinic
2002-2007
BACKGROUNDMany variations in oocyte and embryo grading make inter-laboratory comparisons extremely difficult. This paper reports the proceedings of an international consensus meeting on morphology assessment.
Immuno-surveillance networks operating at barrier sites are tuned by local tissue cues to ensure effective immunity. Site-specific commensal bacteria provide key signals ensuring host defense in the skin and gut. However, how oral microbiome tissue-specific balance immunity regulation gingiva, a barrier, remains minimally explored. In contrast gut, we demonstrate that gingiva-resident T helper 17 (Th17) cells developed via colonization-independent mechanism. Accumulation of Th17 gingiva was...
A correlation between morphology, developmental competence and chromosome abnormalities is established. However, since absolute correlations are rare, embryo selection remains one of the most arduous tasks in assisted reproduction. This study was undertaken order to determine which chromosomal compatible with development blastocyst stage.Embryos diagnosed by preimplantation genetic diagnosis (PGD) as chromosomally abnormal or unsuitable for transfer were cultured day 5 6. Morphology observed...
This study examines the relationship between common morphological anomalies of cleaving embryos and their ability to form apparently normal blastocysts in vitro. The impact cleavage rate, fragmentation, multinucleation on compaction, cavitation, along with inner cell mass trophectoderm formation has been assessed. population consisted 102 patients who elected or were selected have a day 5 embryo transfer. Clinical pregnancy implantation rates 66.7 49% respectively. Slow fast had significant...
A multicentric study was carried out to analyse in a large series: (i) the chromosomal status of unfertilized oocytes, (ii) errors at fertilization and (iii) complement cleaved embryos. Parameters such as type sterility, maternal age, stimulation treatment, doses gonadotrophins administered oocyte preincubation time before insemination were studied relation incidence chromosome abnormalities. Twenty-six per cent oocytes 29.2% embryos had anomalies. Maternal age significantly increased rate...
Fluorescent in-situ hybridization (FISH) of decondensed sperm nuclei has been used directly to evaluate the enrichment efficiency human separation using Sephadex gel filtration and serum albumin (HSA) gradients. Control processed spermatozoa were fixed their decondensed. In-situ was carried out with a Y-specific DNA probe (DYZ1). yielded 52.5% Y-chromosome-bearing spermatozoa, HSA resulted in 49.4% untreated control sample percentage Y 49.3%. Statistical analysis revealed no significant...
The incidence of meiotic abnormalities and their relationship with different spermatogenic parameters was assessed in 103 male patients presumably idiopathic severe oligoasthenozoospermia (motile sperm concentration < or = 1.5 x 10(6)/ml). Meiosis on testicular biopsies independently evaluated by two observers. Meiotic patterns included normal meiosis abnormalities, i.e. arrest synaptic anomalies. A pattern found 64 (62.1%), 21 (20.4%) anomalies 18 (17.5%). overall rate 37.9%. Most (66.7%)...
The developmental potential of early embryos is mainly dictated by the quality oocyte. Here, we explore utility maternal spindle transfer (MST) technique as a reproductive approach to enhance oocyte competence. Our proof-of-concept experiments show that replacement entire cytoplasm oocytes from sensitive mouse strain overcomes massive embryo arrest characteristic non-manipulated oocytes. Genetic analysis confirmed minimal carryover mtDNA following MST. Resulting mice showed low heteroplasmy...
Early embryo freezing with saccharose and propanediol gives a survival rate of 62% the embryos if 4 cell only one intact blastomere after thawing are included. The first pregnancy achieved such an is reported as well preliminary results obtained in our IVF programme using French method.
Which lab-related factors impact the culture system's capacity to maintain a stable osmolality during human embryo culture?Incubator humidity, volume of mineral oil, type media and design time-lapse dishes have been identified as important parameters that can cause an on evaporation consequently culture.Culture medium is critical component in culture. Minimizing its adequate strategy stabilize and, result, improving conditions clinical outcomes.The studied variables included composition...
Journal Article Intracytoplasmic sperm injection versus conventional in-vitro fertilization: first results Get access G. Calderon, Calderon 1 Reproductive Medicine Service, Department of Obstetrics and Gynecology, Instituto Universitario DexeusPaseo Bonanova 69, Barcelona 08017, Spain 1To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar I. Belil, Belil B. Aran, Aran A. Veiga, Veiga Y. Gil, Gil M. Boada, Boada F. Martinez,...
Comparative genomic hybridization (CGH) is a valuable alternative to fluorescence in situ (FISH) for preimplantation genetic screening (PGS) because it allows full karyotype analysis. However, this approach requires the cryopreservation of biopsied embryos until results are available. The aim study reduce period CGH, order make short-CGH technique suitable PGS Day-3 embryos, avoiding step.Thirty-two fibroblasts from six aneuploid cell lines (Coriell) and 48 blastomeres 10 Day-4 discarded...
Techniques of oocyte retrieval have progressed from laparoscopy to transvaginal follicular aspiration under ultrasonographic control. This highly efficient method, routinely used nowadays, is not free complications. We present a case ureteral lesion secondary vaginal ultrasound puncture for recovery in in-vitro fertilization. Despite the surgical procedure reimplant ureter, patient achieved twin pregnancy which ongoing uneventfully.
Previous observations have addressed a decreased male:female ratio associated with smoking. Our aim was to assess whether this effect is observed at the spermatozoa or early embryo development.We retrospectively assessed smoking intake habits of 56 couples included in our preimplantation genetic diagnosis (PGD) program. Three groups were established according male female cigarette consumption per day: non-smokers, smokers (1-19 cigarettes day) and heavy (> =20 day). Fluorescence in-situ...
A twin female pregnancy was obtained in a haemophilia carrier after two preimplantation diagnosis cycles. The embryonic sex of biopsied blastomeres determined with the use dual fluorescence in-situ hybridization directly labelled DNA probes specific for X and Y chromosome. confirmed by means ultrasonography amniocentesis at 14th week amenorrhoea. patient delivered healthy females Caesarian section 37th pregnancy.
Abstract Primers from a recently published study that identified set of low-copy nuclear genes (LCNG) in multiple angiosperms were used to obtain sequence data three LCNG (Chlp, Agt1, and Hmgs) for phylogenetic inference at the species level. The utility each these markers was compared ITS seven chloroplast loci (trnL, trnG-S, ycf5, accD, rpoC1, trnK intron, psbM-trnD intergenic spacer) widely analyses. Here we use Valerianaceae as an example two reasons: 1) group has well-supported...