A5040147449- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Renal and related cancers
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Health and Technologies
- Prenatal Screening and Diagnostics
- Ovarian function and disorders
- Molecular Biology Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Birth, Development, and Health
- 3D Printing in Biomedical Research
- Animal testing and alternatives
- Tissue Engineering and Regenerative Medicine
- Modular Robots and Swarm Intelligence
- Biomedical Ethics and Regulation
- Reproductive System and Pregnancy
- Digital Transformation in Industry
- Pregnancy and preeclampsia studies
- Metabolism and Genetic Disorders
Barcelona Biomedical Research Park
2020-2021
Only 30% of embryos from in vitro fertilized oocytes successfully implant and develop to term, leading repeated transfer cycles. To reduce time-to-pregnancy stress for patients, there is a need diagnostic tool better select based on their physiology. The current standard employs brightfield imaging, which provides limited physiological information. Here, we introduce METAPHOR: Metabolic Evaluation through Phasor-based Hyperspectral Imaging Organelle Recognition. This non-invasive, label-free...
The developmental potential of early embryos is mainly dictated by the quality oocyte. Here, we explore utility maternal spindle transfer (MST) technique as a reproductive approach to enhance oocyte competence. Our proof-of-concept experiments show that replacement entire cytoplasm oocytes from sensitive mouse strain overcomes massive embryo arrest characteristic non-manipulated oocytes. Genetic analysis confirmed minimal carryover mtDNA following MST. Resulting mice showed low heteroplasmy...
Which lab-related factors impact the culture system's capacity to maintain a stable osmolality during human embryo culture?Incubator humidity, volume of mineral oil, type media and design time-lapse dishes have been identified as important parameters that can cause an on evaporation consequently culture.Culture medium is critical component in culture. Minimizing its adequate strategy stabilize and, result, improving conditions clinical outcomes.The studied variables included composition...
Abstract Study question Does application of human stem cell-derived ovarian support cells (OSCs) for in vitro maturation (IVM) have a safe reproductive toxicity profile? Summary answer The use OSC-IVM co-culture improves blastocyst formation mouse model and results healthy live births with no evidence reprotoxicity. What is known already Abbreviated stimulation to obtain immature oocytes combined successful IVM offers promising alternative traditional fertilization, reducing hormonal doses...
Are there significant differences between the available commercial oil brands used for human IVF?Important have been detected among tested in their potential to stabilize culture conditions and, more importantly, direct effect on embryo development and viability.Mineral is a critical component of system due its protective stabilizing roles during vitro development. Many different oils are market, with viscosity, density overall quality.Thirteen were compared.Each was firstly analyzed assess...
To study the effect of zona opening (ZO) and 2 thinning (ZT) techniques on hatching process mouse embryos using a last-generation laser system time-lapse microscopy (TLM).Prospective randomized study.Private research center.A total 267 F1 hybrid (B6/CBA) mice were included.Morulae randomly selected pellucida (ZP) manipulated according to 4 experimental groups: control (ZP intact, n = 59), ZO (25 μm hole, 70), ZT25 (25% perimeter thinned, 71), ZT35 (35% 67). Embryo development was monitored...
(Abstracted from Reprod Biomed Online 2023;47:103237 Intracytoplasmic sperm injection (ICSI) is a common procedure used in approximately 70% of vitro fertilization (IVF) cycles. The involves using glass micropipettes to penetrate the oolemma and into oocyte.
Abstract Study question Can intracytoplasmic sperm injection (ICSI) be performed remotely using a personal computer and an intelligent robotic micromanipulation system? Summary answer An AI-enhanced system was developed that allows successful remote execution of ICSI by experts or non-experts. What is known already has become the method choice for fertilization eggs during IVF, but it remains technically demanding, requiring manual control highly specialized equipment expert embryologists....
Background: Intracytoplasmic sperm injection (ICSI) is a laboratory procedure that has been conducted in most IVF centers by highly experienced embryologists manually. Since it involves micromanipulation and technically demanding, its successful rates may vary significantly depending on the experience of each operator. Here, we developed an automated ICSI (ICSIA) robot requires minimal manual intervention with aim to standardize consistency technique clinical results carried out...
Abstract Study question To classify embryos according to their metabolic fingerprint with a robust, non-invasive methodology Summary answer Our Hyperspectral (HS) phasor analysis coupled artificial intelligence simultaneously detects 6+ metabolites profile and competent oocytes safely consistently. What is known already Metabolism plays key role in oocyte embryo developmental competence. However, current methods measure metabolism are either invasive, phototoxic, too slow or based on...