A5064712625- Cannabis and Cannabinoid Research
- Stress Responses and Cortisol
- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Neuroscience and Neuropharmacology Research
- Multiple Sclerosis Research Studies
- Neuropeptides and Animal Physiology
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Immune Response and Inflammation
- Neuroendocrine regulation and behavior
- Immune Cell Function and Interaction
- Neurogenesis and neuroplasticity mechanisms
- Adrenal Hormones and Disorders
- Neuroscience of respiration and sleep
- Hormonal Regulation and Hypertension
- Sleep and Wakefulness Research
- Gut microbiota and health
- Nitric Oxide and Endothelin Effects
- Histone Deacetylase Inhibitors Research
- Phosphodiesterase function and regulation
- Pancreatic function and diabetes
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Immune cells in cancer
Instituto Cajal
2013-2022
Spanish Multiple Sclerosis Network
2013-2021
ORCID
2020-2021
Instituto de Salud Carlos III
2013-2021
Weatherford College
2008-2020
Cordoba University
2020
University of Córdoba
2020
Consejo Superior de Investigaciones Científicas
2005-2017
Instituto Ramón y Cajal de Investigación Sanitaria
2015
Karolinska Institutet
2013
Abstract Stress‐related adrenal steroid hormones modulate brain and cognitive function. Electrophysiological studies, including primed burst potentiation long‐term potentiation, have indicated concentration‐dependent inverted U‐shape effects of corticosterone in hippocampal function plasticity. Here, we explored the role consolidation retrieval spatial learning Morris water maze task rats. We postulated that actions might be experience‐dependent with regard to stimulus intensity, such as...
Cannabinoids exert pleiotropic actions in the CNS, including inhibition of inflammatory responses and enhancement neuronal survival after injury. Although cannabinoid receptors are distributed widely brain, their presence has not been investigated previously oligodendrocytes. This study examined expression type 1 (CB1) rat oligodendrocytes vivo culture explored biological function. Expression CB1 by was demonstrated immunocytochemically postnatal adult white matter as well oligodendrocyte...
Inflammation in the central nervous system (CNS) is a complex process that involves multitude of molecules and effectors, it requires transmigration blood leukocytes across blood-brain barrier (BBB) activation resident immune cells. Cannabidiol (CBD), non-psychotropic cannabinoid constituent Cannabis sativa, has potent anti-inflammatory immunosuppressive properties. Yet, how this compound modifies deleterious effects inflammation TMEV-induced demyelinating disease (TMEV-IDD) remains unknown....
Abstract Deregulation of glycogen synthase kinase‐3 (GSK‐3) activity in neurones has been postulated as a key feature Alzheimer's disease (AD) pathogenesis. This was further supported by our recent characterization transgenic mice that conditionally over‐express GSK‐3β hippocampal and cortical neurones. These mice, designated Tet/GSK‐3β, showed many the biochemical cellular aspects AD neuropathology such tau hyperphosphorylation somatodendritic localization, decreased nuclear β‐catenin,...
Theiler's virus infection of the CNS induces an immune-mediated demyelinating disease in susceptible mouse strains and serves as a relevant model for human multiple sclerosis (MS). Cannabinoids may act immunosuppressive compounds that have shown therapeutic potential chronic inflammatory disorders. Using murine encephalomyelitis model, we report here treatment with synthetic cannabinoids WIN 55,212–2, ACEA, JWH-015 during established significantly improved neurological deficits long-lasting...
Interleukin‐1 (IL‐1) is an important mediator of immunoinflammatory responses in the brain. In present study, we examined whether prostaglandin E 2 (PGE ) production after IL‐1β stimulation dependent upon activation protein kinases astroglial cells. Astrocyte cultures stimulated with or phorbol ester, PMA significantly increased PGE secretion. The stimulatory action on was totally abolished by NS‐398, a specific inhibitor cyclo‐oxygenase‐2 activity, as well synthesis cycloheximide, and...
Glucocorticoids modulate brain function and behaviour through different mechanisms. Although classical effects are mediated intracellular receptors that gene transcription, recent evidence supports the existence of rapid, nongenomic steroid neuronal membrane. In this study, we explored possible rapid behavioural corticosterone in rat, which could provide a model to characterize further mechanisms involved corticosteroid actions. We found injection, at doses (2.5 or 5 mg/kg) mimic plasma...
Abstract The endocannabinoid system exhibits anti‐inflammatory properties by regulating cytokine production. Anandamide (AEA) down‐regulates proinflammatory cytokines in a viral model of multiple sclerosis (MS). However, little is known about the mechanisms which AEA exerts these effects. Microglial cells are main source within brain and first barrier defense against pathogens acting as antigen presenting cells. IL‐10 key physiological negative regulator microglial activation. In this study...
Extracellular vesicles (EVs) have emerged as important mediators of intercellular communication and possible therapeutic agents in inflammation-mediated demyelinating diseases, including multiple sclerosis (MS). In the present study, we investigated whether intravenously administered EVs derived from mesenchymal stem cells (MSCs) human adipose tissue might mediate recovery Theiler's murine encephalomyelitis virus (TMEV)-induced disease, a progressive model MS. SJL/J mice were subjected to EV...
Cannabidiol (CBD) is the most abundant cannabinoid in Cannabis sativa that has no psychoactive properties. CBD been approved to treat inflammation, pain and spasticity associated with multiple sclerosis (MS), of which demyelination oligodendrocyte loss are hallmarks. Thus, we investigated protective effects against damage progenitor cells (OPCs) mediated by immune system. Doses 1 μM protect OPCs from oxidative stress decreasing production reactive oxygen species. also protects apoptosis...
Abstract The endocannabinoid anandamide (AEA) is released by macrophages and microglia on pathological neuroinflammatory conditions such as multiple sclerosis (MS). CD200 a membrane glycoprotein expressed in neurons that suppresses immune activity via its receptor (CD200R) mainly located macrophages/microglia. CD200‐CD200R interactions contribute to the brain privileged status. In this study, we show AEA protects from microglia‐induced neurotoxicity interaction. increases expression of...
Interleukin-1 receptor antagonist (IL-1ra) is an important anti-inflammatory cytokine that blocks all known actions of IL-1 and markedly protects against experimentally induced ischemic, excitotoxic, traumatic brain insults. Cannabinoids (CBs) also exert potent neuroprotective effects, but the mechanisms their are unknown. Here we tested hypothesis CBs mediated by endogenous IL-1ra. We report for first time both CB 1 2 receptors modulate release IL-1ra from primary cultured glial cells....
Abstract Proinflammatory mediators have been implicated in demyelinating disorders, including multiple sclerosis, whereas it has proposed that the anti‐inflammatory cytokines interleukin‐ (IL‐) 4 and IL‐10 participate disease recovery. The present study analysed effect of interferon‐γ (IFN‐γ) bacterial endotoxin (lipopolysaccharide, LPS) on proliferation survival progenitors differentiated oligodendrocytes. We also investigated presence receptors for IL‐4 oligodendroglial cells explored a...
Abstract The purpose of this study was to investigate the role central cannabinoid receptor (CB 1 ) in mediating actions endogenous agonist anandamide and synthetic CP‐55940. Activation primary mouse astrocyte cultures by exposure bacterial lipopolysaccharide (LPS) caused a marked (approximately tenfold) increase nitric oxide (NO) release. Coincubation with agonists or CP‐55940 markedly inhibited release NO (–12% –55%). This effect abolished SR‐141716A (1 μM), CB antagonist. alone also...
We have studied the possible interaction between cannabinoid receptor agonist CP 55,940 (1 and 50 μg/kg) 5-HT1A antagonist WAY 100635 mg/kg) in modulation of plus-maze holeboard activity Wistar adult male rats. In plus-maze, higher dose induced an anxiogenic-like effect, whereas lower anxiolytic-like responses. The antagonist, which was silent this test, attenuated anxiogenic, but not anxiolytic, effect 55,940. holeboard, significantly decreased head-dipping duration, 100635, did affect...
Abstract Theiler's virus infection of the central nervous system (CNS) induces an immune‐mediated demyelinating disease in susceptible mouse strains and serves as a relevant model for human multiple sclerosis (MS). Cannabinoids have been shown to exert beneficial effects on animal models MS evidence suggests that endocannabinoid plays role tonic control spasticity. In this study we show OMDM1 [( R )‐ N ‐oleoyl‐(1′‐hydroxybenzyl)‐2′‐ethanolamine] OMDM2 S...
Cannabinoid (CB) receptor agonists have potential utility as anti‐inflammatory drugs for the treatment of many disease conditions. In present study, we investigated effects synthetic CB 2 ligand, JWH‐133 on production interleukins (ILs), IL‐12 and IL‐10 by lipopolyssacharide (LPS) or Theiler's virus (TMEV)‐activated macrophages. evoked a concentration‐related inhibition (10 n M –5 μ ) LPS/IFN‐γ induced IL‐12p40 release. The effect (100 was significantly blocked antagonist SR‐144528 (1 )....