A5090690141- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Curcumin's Biomedical Applications
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Oral health in cancer treatment
- Endodontics and Root Canal Treatments
- Additive Manufacturing and 3D Printing Technologies
- Hedgehog Signaling Pathway Studies
- Antimicrobial Peptides and Activities
- Skin Protection and Aging
- 3D Printing in Biomedical Research
- Exercise and Physiological Responses
- Microplastics and Plastic Pollution
- melanin and skin pigmentation
- Mesenchymal stem cell research
- Nanoparticles: synthesis and applications
- Peptidase Inhibition and Analysis
- Synthesis and bioactivity of alkaloids
- Social and Political Issues
- Laser Applications in Dentistry and Medicine
- Advancements in Transdermal Drug Delivery
- Food Safety and Hygiene
- Cystic Fibrosis Research Advances
- Microbial Metabolism and Applications
Universidade Federal do Rio Grande do Sul
2017-2025
Vitrogen (Brazil)
2024
University of Rio Grande and Rio Grande Community College
2023
University of California, San Diego
2018-2019
La Jolla Bioengineering Institute
2019
La Jolla Alcohol Research
2018
Health First
2018
University of Michigan
2013-2015
Michigan United
2015
The use of small antimicrobial peptides or bacteriocins, like nisin, to treat cancer is a new approach that holds great promise. Nisin exemplifies this because it has been used safely in humans for many years as food preservative, and recent laboratory studies support its anti-tumor potential head neck cancer. Previously, we showed nisin (2.5%, low content) antitumor squamous cell carcinoma (HNSCC) vitro vivo. current explored naturally occurring variant (nisin ZP; 95%, high effects ZP...
Significance Gradual stiffening of the native extracellular matrix has been implicated in promoting breast cancer progression through stiffness-mediated signaling. Herein, we probe mammary epithelial cell responses to substrate stiffness with a dynamically stiffened hydrogel, enabling investigation how change impacts collective behaviors. We demonstrate that signaling pathways, both paracrine and mechanosensing, impact response act concert drive epithelial-mesenchymal plasticity....
Tumors are composed of heterogeneous phenotypes, each having different sensitivities to the microenvironment. One microenvironment characteristic–matrix stiffness–helps regulate malignant transformation and invasion in mammary tumors, but its influence on oral squamous cell carcinoma (OSCC) is unclear. We observed that stiff matrices, a highly invasive OSCC line with low E-cad/N-cad ratio (InvH/E:NL; SCC25) had increased migration velocity decreased adhesion strength compared poorly high...
One of the most frequent causes respiratory infections are viruses. Viruses reaching airways can be absorbed by human body through mucosa and mainly infect lung cells. Several viral not yet curable, such as coronavirus-2 (SARS-CoV-2). Furthermore, side effect synthetic antiviral drugs reduced efficacy against resistant variants have reinforced search for alternative effective treatment options, plant-derived molecules. Curcumin (CUR) quercetin (QUE) two natural compounds that been widely...
Matrix stiffening is ubiquitous in solid tumors and can direct epithelial-mesenchymal transition (EMT) cancer cell migration. Stiffened niche even cause poorly invasive oral squamous carcinoma (OSCC) lines to acquire a less adherent, more migratory phenotype, but mechanisms durability of this acquired "mechanical memory" are unclear. Here, we observed that contractility its downstream signals could underlie memory acquisition; SSC25 cells overexpress myosin II (vs. noninvasive Cal27 cells)...
Calcium hydroxylapatite (CaHA) is widely used in aesthetics for its dual role as a dermal filler and biostimulator, promoting collagen elastin synthesis. This study evaluates the comparative potential of CaHA conventional saline dilution versus mixed with poly-micronutrient solution (CaHA/PMN) to enhance biostimulatory effects. In an vitro model, primary human fibroblasts were treated both formulations, cell viability gene expression type I collagen, elastin, FOXO3, SIRT-1, SIRT-3 assessed....
Zanthoxylum rhoifolium is used in folk medicine as an antiparasitic agent. Therefore, this study evaluated the phytochemical aspects and biological activities of Z. rhoifolium. For this, ethanolic extract (EE) was obtained by macerating peel with ethanol subjected to acid-base partition obtain neutral fractions (FN) alkaloid (FA). These samples were analyzed using chromatography techniques. From a substance isolated from FN identified nuclear magnetic resonance. activity, strains Leishmania...
Cell invasion and metastasis are involved in clinical failures cancer treatment, both events require the acquisition of a migratory behavior by tumor cells. Curcumin is promising natural product with anti‐proliferative activity, but its effects on cell migration still unclear. We evaluated curcumin proliferation, apoptosis, migration, cell–cell adhesion keratinocyte, oral squamous carcinoma (OSCC), fibroblast lines, as well xenograft model OSCC. (2 μM) decreased proliferation lines...
Abstract CD 44, an extracellular matrix ( ECM ) receptor, has been described as a cancer stem cell marker in multiple cancers, including head and neck squamous carcinoma HNSCC ). orasphere formation or stemness was characterized by cleavage of thus we hypothesized that this proteolytic processing may be critical to tumorigenesis. We tested hypothesis examining the mechanisms regulate process vitro vivo, exploring its clinical relevance human specimens. Sphere assays have used evaluate vitro....
Background Information Cell migration requires the coordinated activation of structural and signalling molecules, such as RhoGTPase Rac1. It is known that nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex assembly, which generates reactive oxygen species (ROS) at cell membrane, also relies on Rac1 activation, indicating a possible effect ROS during migration. In this study, we evaluated NADPH‐oxidase‐derived process. Results Using time‐lapse videos CHO.K1 cells plated...
Abstract The use of small antimicrobial agents or bacteriocins, like nisin, to treat cancer is a new approach that holds great promise. Nisin exemplifies this because it has been used safely in humans for many years as food preservative, and recent laboratory studies support its anti-tumor potential head neck cancer. Previously, we showed nisin (2.5%, low content) antitumor squamous cell carcinoma (HNSCC) vitro vivo. current explored naturally occurring variant (nisin ZP; 95%, high effects...
Considering that microRNAs (miRNAs), extracellular vesicles and particles (EVPs) the amyloid precursor protein (APP) processing have been shown to be altered in oral squamous cells carcinoma (OSCC), it is possible miRNAs target APP pathways EVPs are impacted tumor cells. Our aim was evaluate itself or disintegrin metalloproteinase domain 10 (ADAM10), which generate a trophic compound, sAPPα, derived from OSCC cell lines, an aggressive non-invasive, compared normal keratinocytes.We used two...
Background: Skin pigmentation is a complex process; thus, skin equivalent methodologies that can reproduce the main structures and have been studied. To improve model, bioprinting technology has emerged, allowing for reproduction of complex, functional, personalized three-dimensional architecture skin. Objective: Our aim was to develop model pigmented compare manually produced models with bioprinted models. Methods: The study conducted using fibroblasts, keratinocytes, melanocytes cell lines...
Background: Skin aging is a result of the process and also intrinsic extrinsic factors. In order to better understand this evaluate anti-aging products, it necessary develop in vitro models that can recapitulate biological aged skin. Therefore, aim study was an skin equivalent model these properties. Methods: Human fibroblasts were incorporated into collagen matrix keratinocytes added cultured air-liquid interface for 21 days. During period, matrices exposed UV or EX527 trigger processes....