A5075040196- Mesenchymal stem cell research
- Nerve injury and regeneration
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Wound Healing and Treatments
- Oral microbiology and periodontitis research
- Lipid metabolism and disorders
- Diabetes Treatment and Management
- Diabetic Foot Ulcer Assessment and Management
- Lipoproteins and Cardiovascular Health
- Bone and Dental Protein Studies
- Salivary Gland Disorders and Functions
- Cancer, Lipids, and Metabolism
- Pancreatic function and diabetes
- Peptidase Inhibition and Analysis
- Advanced Glycation End Products research
- Cancer-related molecular mechanisms research
- Antiplatelet Therapy and Cardiovascular Diseases
- Neuropeptides and Animal Physiology
- Pain Mechanisms and Treatments
- Axon Guidance and Neuronal Signaling
- 3D Printing in Biomedical Research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Diet and metabolism studies
- Pharmacology and Obesity Treatment
- Nutritional Studies and Diet
Tokushima University
2025
Aichi Gakuin University
2012-2024
Nagoya City University
2024
Toyota Central Research and Development Laboratories (Japan)
2018-2020
Nagoya University
1994-2010
Kumamoto University
1979-1988
OBJECTIVE—Mesenchymal stem cells (MSCs) have been reported to secrete various cytokines that exhibit angiogenic and neurosupportive effects. This study was conducted investigate the effects of MSC transplantation on diabetic polyneuropathy (DPN) in rats. RESEARCH DESIGN AND METHODS—MSCs were isolated from bone marrow adult rats transplanted into hind limb skeletal muscles with an 8-week duration streptozotocin (STZ)-induced diabetes or age-matched normal by unilateral intramuscular...
Abstract Aims/Introduction Dental pulp stem cells ( DPSC s) are thought to be an attractive candidate for cell therapy. We recently reported that the transplantation of s increased nerve conduction velocity and blood flow in diabetic rats. In present study, we investigated immunomodulatory effects on peripheral nerves. Materials Methods were isolated from dental Sprague–Dawley rats expanded culture. Eight weeks after streptozotocin injection, transplanted into unilateral hindlimb skeletal...
A relationship between periodontal disease and atherosclerosis has been suggested by epidemiological studies. Ligature-induced experimental periodontitis is an adequate model for clinical periodontitis, which starts from plaque accumulation, followed inflammation in the tissue. Here we have demonstrated using a ligature-induced that activates monocytes/macrophages, subsequently circulate blood adhere to vascular endothelial cells without altering serum TNF-α concentration. Adherent...
Relief from painful diabetic neuropathy is an important clinical issue. We have previously shown that the transplantation of cultured endothelial progenitor cells or mesenchymal stem ameliorated in rats. In this study, we investigated whether freshly isolated bone marrow-derived mononuclear (BM-MNCs) alleviates neuropathic pain early stage streptozotocin-induced Two weeks after STZ injection, BM-MNCs vehicle saline were injected into unilateral hind limb muscles. Mechanical hyperalgesia and...
The aim of this study was to assess the efficacy a self-assembling peptide hydrogel as scaffold for bone regeneration. We used neutral and injectable hydrogel, SPG-178-Gel. Bone defects (5 mm in diameter) rat calvarial bones were filled with mixture alpha-modified Eagle's medium hydrogel. Three weeks after surgery, soft X-ray microcomputed tomography (micro-CT) images gel-treated showed new formations periphery central areas defects. Next, we evaluated three-dimensional osteogenic induction...
Although previous reports have revealed the therapeutic potential of stem cell transplantation in diabetic polyneuropathy, effects on long-term polyneuropathy not been investigated. In this study, we investigated whether dental pulp cells (DPSCs) ameliorated streptozotocin (STZ)-induced rats. Forty-eight weeks after STZ injection, transplanted DPSCs into unilateral hindlimb skeletal muscles. Four DPSC (i.e., 52 injection) were assessed. STZ-induced rats showed significant reductions sciatic...
Dental pulp stem cells (DPSCs), which can differentiate into several types of cells, are subjected to mechanical stress by jaw movement and occlusal forces. In this study, we evaluated how the uniaxial stretch influences proliferation differentiation DPSCs. DPSCs were isolated cultured from male Sprague-Dawley rats. Cultured identified surface markers capabilities as adipocytes or osteoblasts. To examine response stress, was exposed We impact on intracellular signaling, proliferation,...
Abstract Introduction Dental pulp stem cells (DPSCs) are mesenchymal located in dental and thought to be a potential source for cell therapy since DPSCs can easily obtained from teeth extracted orthodontic reasons. Obtained cryopreserved until necessary thawed expanded when needed. The aim of this study is evaluate the therapeutic DPSC transplantation diabetic polyneuropathy. Methods isolated incisors Sprague–Dawley rats were partly frozen −80 °C freezer 6 months. Cultured transplanted into...
Dental pulp stem cells (DPSCs) can be easily obtained from teeth for general orthodontic reasons. We have previously reported the therapeutic effects of DPSC transplantation diabetic polyneuropathy. As abundant secretomes DPSCs are considered to play a central role in improvement polyneuropathy, we investigated whether direct injection DPSC-conditioned media (DPSC-CM) into hindlimb skeletal muscles ameliorates polyneuropathy rats.DPSCs were isolated dental Sprague-Dawley rats. Eight weeks...
Dental pulp stem cells (DPSCs) have high proliferation and multi-differentiation capabilities that maintain their functionality after cryopreservation. In our previous study, we demonstrated cryopreserved rat DPSCs improved diabetic polyneuropathy the efficacy of was equivalent to freshly isolated DPSCs. The present study conducted evaluate whether transplantation human (hDPSCs) is also effective for treatment polyneuropathy. hDPSCs were from impacted third molars being extracted orthodontic...
β-Aminoisobutyric acid (BAIBA) is a myokine that secreted from skeletal muscles by the exercise. Recently, increasing evidence has suggested multifocal physiological activities of BAIBA. In this study, we investigated whether L-BAIBA protective effects on rat pheochromocytoma (PC12) cells. Cultured PC12 cells were stimulated with L-BAIBA. Western blot analyses revealed stimulation significantly increased phosphorylation AMPK and Akt. contrast, no effect was observed neurite outgrowth To...
Abstract Aims/Introduction The involvement of glucose‐dependent insulinotropic polypeptide ( GIP ) on inflammation was explored in atherosclerosis and adipose tissue. Periodontal disease is a chronic inflammatory disease, considered one the diabetic complications. In present study, to examine effect periodontitis, we induced experimental periodontitis receptor‐knockout mice GIPRKO ). We also investigated anti‐inflammatory culture system. Materials Methods Experimental by ligature wire C57 BL...
Transplantation of stem cells promotes axonal regeneration and angiogenesis in a paracrine manner. In the present study, we examined whether secreted factors conditioned medium from human exfoliated deciduous teeth (SHED-CM) had beneficial effects on diabetic polyneuropathy mice.
Diabetes is a major risk factor for atherosclerosis and ischemic vascular diseases. Recently, regenerative medicine expected to be novel therapy Our previous studies have reported that transplantation of stem cells promoted therapeutic angiogenesis diabetic neuropathy disease in paracrine manner, but the precise mechanism unclear. Therefore, we examined whether secreted factors from had direct beneficial effects on endothelial promote angiogenesis. The soluble were collected as conditioned...
Stem cell transplantation is a potential novel therapy for diabetic polyneuropathy. Dental pulp stem cells (DPSCs) are attractive sources because DPSCs can be isolated from extracted teeth and cryopreserved while retaining viability. In this study, we directly compared the efficacy of administration secreted factors (DPSC-SFs) on Eight weeks after streptozotocin injection, (1.0 × 106 cells/rat) or DPSC-SFs mL/rat) were administered into unilateral hindlimb skeletal muscles Sprague–Dawley...
Abstract Aims To investigate the effect of smoking on soluble adhesion molecules in middle‐aged diabetic patients. Methods One hundred out‐patients with Type 2 diabetes and 100 age‐ sex‐matched non‐diabetic subjects without clinical macrovascular disease were selected. Soluble serum levels analysed using enzyme immunoassay. Carotid atherosclerosis was assessed an ultrasound system. Results When compared subjects, vascular cell molecule‐1 (sVCAM‐1), intercellular (sICAM‐1), sE‐selectin found...
Dental pulp stem cells (DPSCs) are suitable for use in regenerative medicine. Cryopreserved human DPSCs (hDPSCs) ameliorate diabetic polyneuropathy, and the effects of hDPSC transplantation related to VEGF NGF secretion. This study evaluated long-term a single hDPSCs on polyneuropathy. were obtained from third molars extracted orthodontic treatment, which then transplanted into unilateral hindlimb skeletal muscles 8 weeks after streptozotocin injection nude mice. The analyzed at 16...