A5076044078- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Ion Transport and Channel Regulation
- Receptor Mechanisms and Signaling
- Hormonal Regulation and Hypertension
- Microtubule and mitosis dynamics
- Cardiac Arrhythmias and Treatments
- Cellular transport and secretion
- Protein Kinase Regulation and GTPase Signaling
- Nitric Oxide and Endothelin Effects
- Renin-Angiotensin System Studies
- Tea Polyphenols and Effects
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Atherosclerosis and Cardiovascular Diseases
- Heart Rate Variability and Autonomic Control
University of Copenhagen
2019-2024
Aarhus University
2023
University of Ljubljana
2023
Tallinn University of Technology
2023
BACKGROUND/AIMS: Tea, produced from the evergreen Camellia sinensis, has reported therapeutic properties against multiple pathologies, including hypertension. Although some studies validate health benefits of tea, few have investigated molecular mechanisms action. The KCNQ5 voltage-gated potassium channel contributes to vascular smooth muscle tone and neuronal M-current regulation. METHODS: We applied electrophysiology, myography, mass spectrometry in silico docking determine effects their...
The dynein motor protein transports proteins away from the cell membrane along microtubule network. Recently, we found network was important for regulating abundance of voltage-gated Kv7.4 potassium channels in vascular smooth muscle. Here, aimed to investigate influence on microtubule-dependent internalization channel. Patch-clamp recordings HEK293B cells showed currents were increased after inhibiting function with ciliobrevin D or by coexpressing p50/dynamitin, which specifically...
Background: The voltage-gated potassium channel (Kv)7.4 and Kv7.5 channels contribute to the β-adrenoceptor-mediated vasodilatation. In arteries from hypertensive rodents, Kv7.4 is downregulated function attenuated, which contributes reduced vasodilatation observed in these arteries. Recently, we showed that disruption of microtubule network, with colchicine, or inhibition motor protein, dynein, ciliobrevin D, enhanced membrane abundance rat mesenteric This study aimed determine whether...
Background: Several local Ca 2+ events are characterized in smooth muscle cells. We have previously shown that an inhibitor of the Na,K-ATPase, ouabain induces spatially restricted intracellular transients near plasma membrane, and suggested importance this signaling for regulation intercellular coupling cell contraction. The mechanism behind these Na,K-ATPase-dependent “Ca flashes” remains to be elucidated. In addition its conventional ion transport function, Na,K-ATPase is proposed...
Abstract Objective About one third of all patients with epilepsy have pharmacoresistant seizures. Thus there is a need for better pharmacological treatments. The human voltage‐gated potassium (hK V ) channel hK 7.2/7.3 validated antiseizure target compounds that activate this channel. In previous study we shown resin acid derivatives can the investigated if these activators potential to be developed into new type drug. examined their structure‐activity relationships and site action on...
Abstract Colchicine, a microtubule depolymerizing agent, is an effective therapy for the secondary prevention of cardiovascular disease and has been approved recently as novel treatment atherosclerosis associated with coronary artery disease. Hypertension leading cause disease, yet impact colchicine on hypertension not studied. We hypothesized that low-dose could be used to treat reduce risk. The aim this study was administer daily, low dose (0.05 mg/kg/day) oral 4 weeks spontaneously...
Abstract Large‐conductance, calcium‐activated potassium channels (BK channels) and the Na/K‐ATPase are expressed universally in vascular smooth muscle. The may act via changes intracellular Ca 2+ concentration mediated by Na/Ca exchanger (NCX) Src kinase. Both pathways known to regulate BK channels. Whether functionally interact muscle cells with remains be elucidated. Thus, this study addressed hypothesis that limit ouabain‐induced vasocontraction. Rat mesenteric arteries were studied using...
Abstract Prorenin and the prorenin receptor ((P)RR) are important, yet controversial, members of renin–angiotensin–aldosterone system. The is expressed throughout body, including vasculature, however, direct effect on arterial contractility to be determined. Within rat mesenteric arteries, immunostaining proximity ligation assays were used determine interacting partners (P)RR in freshly isolated vascular smooth muscle cells (VSMCs). Wire myography examined functional prorenin. Simultaneous...
<b>Abstract ID 14432</b> <b>Poster Board 102</b> In the USA, resistant uncontrolled hypertensive patients express an increased risk of mortality when compared to untreated patients<sup>1</sup>. The current pharmacopeia medication therefore fails ameliorate hypertension and poses a health patients, highlighting need for new drugs change in approach how we treat hypertension. Colchicine, microtubule polymerization disruptor, is currently use its anti-inflammatory properties treatment gout....
Introduction: Sortillin-related VPS10P containing receptor (SorCS2) is expressed in neurons and other tissues during development where it mediates trafficking of target proteins between cell membrane intercellular compartments.It has also been suggested that SorCS2 modulates signal transduction.SorCS2 upregulated vascular wall under pathological conditions, e.g., atherosclerosis.The aim this study to elucidate the contribution endothelial smooth muscle function.Method: We compared mesenteric...