Endo-dormant flower buds must pass through a period of chilling to reinitiate growth and subsequent flowering, which is major obstacle the forcing culture tree peony in winter. Customized cDNA microarray (8×15 K element) was used investigate gene expression profiling 'Feng Dan Bai' during 24 d treatment at 0–4°C. According morphological changes after whole plants were transferred green house, endo-dormancy released 18 treatment, prolonged increased bud break rate. Pearson correlation...

Sufficient low temperature accumulation is the key strategy to break bud dormancy and promote subsequent flowering in tree peony anti-season culturing production. Exogenous gibberellins (GAs) could partially replace chilling accelerate release, different kinds of GAs showed inconsistent effects various plants. To understand exogenous GA3 GA4 on release growth, morphological changes were observed after applications, differentially expressed genes (DEGs) identified, contents endogenous...

Abstract Tree peony bud endodormancy is a common survival strategy similar to many perennial woody plants in winter, and the activation of GA signaling pathway key breaking endodormancy. signal transduction involved physiological processes. Although GA-GID1-DELLA regulatory module conserved plants, it has set specific components that add complexity response mechanism. DELLA proteins are switches signaling. Therefore, there an urgent need identify tree dormancy release. In this study,...

Abstract MicroRNAs (miRNAs) are non-coding RNAs that interact with target genes and involved in many physiological processes plants. miR172-AP2 mainly plays a role the regulation of flowering time floral organ differentiation. Bud dormancy release is necessary for forcing culture tree peony winter, but mechanism unclear. In this study, we found miR172 family member, PsmiR172b, was downregulated during chilling-induced bud peony, exhibiting trend opposite to PsTOE3. RNA ligase-mediated (RLM)...

Tree peony, one of the most valuable horticultural and medicinal plants in world, has to go through winter break dormancy. Growing studies from molecular aspects on dormancy release process have been reported, but inadequate study done miRNA-guided regulation tree peony. In this study, high-throughput sequencing was employed identify characterize miRNAs three libraries (6 d, 18 d 24 chilling treatments). There were 7,122, 10,076 9,097 unique miRNA sequences belonging 52, 87 68 families,...

Bud dormancy, which serves as a survival mechanism during winter, is crucial for determining the timing and quality of flowering in many perennial woody plants, including tree peony. The gibberellin (GA) signaling pathway participates breaking bud dormancy Specifically, PsRGL1, key DELLA protein, negative regulator this process. MADS-box family members participate plant growth development regulation. In study, MADS-domain transcription factor, AGAMOUS-LIKE 9 (PsAGL9), was identified...

Abstract Background Bud dormancy is a sophisticated strategy which plants evolve to survive in tough environments. Endodormancy key obstacle for anti-season culture of tree peony, and sufficient chilling exposure an effective method promote release perennial including peony. However, the mechanism still poorly understood, there are few systematic studies on metabolomics during induced transition. Results The peony buds were treated with artificial chilling, metabolmics analysis was employed...

Summary Tree peony ( Paeonia suffruticosa ) undergoes bud endodormancy, and gibberellin (GA) pathway plays a crucial role in dormancy regulation. Recently, key DELLA protein PsRGL1 has been identified as negative regulator of release. However, the mechanism GA signal to break remains unknown. In this study, yeast two‐hybrid screened PsSOC1 interacting with through its MADS domain, interaction was using pull‐down luciferase complementation imaging assays Transformation tree hybrid poplar...

The MPT transports Pi to synthesize ATP. PsMPT, a chilling-induced gene, was previously reported promote energy metabolism during bud dormancy release in tree peony. In this study, the regulatory elements of PsMPT promoter involved chilling response were further analyzed. transcript detected different peony tissues and highly expressed flower organs, including petal, stigma stamen. An 1174 bp isolated by TAIL-PCR, promoter::GUS transgenic Arabidopsis generated GUS staining qPCR showed that...

Abstract Bud endodormancy in perennial plants is a sophisticated system that adapts to seasonal climatic changes. Growth-promoting signals such as low temperature and gibberellins (GAs) are crucial for facilitating budbreak following release (EDR). However, the regulatory mechanisms underlying GA-mediated tree peony (Paeonia suffruticosa) remain unclear. In peony, expression of PsmiR159b among three differentially expressed miR159 members was inhibited with prolonged chilling, overexpression...

The brief longevity of tree peony blossoms constrains its ornamental value and economic worth. Gibberellins (GAs) are crucial in the modulation flower senescence, GA 20-oxidase (GA20ox), 3-oxidase (GA3ox), 2-oxidase (GA2ox) catalyze synthesis deactivation bioactive GAs. In Paeonia ostii, a total three PoGA20ox, ten PoGA3ox, twelve PoGA2ox proteins were identified comprehensively analyzed. analysis gene structures, conserved domains, motifs revealed structural similarities variances among...

Endo-dormant flower buds of tree peony must have sufficient chilling duration to reinitiate growth, which is a major obstacle the forcing culture in winter. We used combination two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionisation time flight/time flight mass spectrometry (MALDI-TOF/TOF MS) identify differentially expressed proteins after three different treatments: endo-dormancy, endo-dormancy release eco-dormancy stages. More than 200 highly...

As a crop irrigated primarily by rain, the quality and yield of peanuts are significantly limited drought. To date, many studies have indicated that fatty acid desaturase (FAD) genes enhance plant tolerance to drought stresses. In this study, 16, 15, 31 FADs were identified in Arachis duranensis, ipaensis, hypogaea, respectively. All divided into four subfamilies, which had relatively conserved gene structures, motifs, domains. The synteny relationships chromosomal position analysis showed...

Cotton is not only a major textile fiber crop but also vital oilseed, industrial, and forage crop. Oleosins are the structural proteins of oil bodies, influencing their size content in seeds. In addition, degradation oleosins involved mobilization lipid bodies during seed germination. However, comprehensive identification systematic analysis Oleosin gene (OLEOs) family have been conducted cotton.An in-depth has enabled us to identify 25 24 OLEOs tetraploid cotton species G. hirsutum...