A5083760987- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Genomics and Phylogenetic Studies
- Fungal Biology and Applications
- Plant Pathogens and Resistance
- Plant Disease Resistance and Genetics
- Fermentation and Sensory Analysis
- Environmental DNA in Biodiversity Studies
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Microbial Natural Products and Biosynthesis
- Yeasts and Rust Fungi Studies
- Bee Products Chemical Analysis
- Microbial Community Ecology and Physiology
- Bacterial Identification and Susceptibility Testing
- Enzyme Production and Characterization
- Microbial Metabolism and Applications
Bavarian State Research Center for Agriculture
2020-2022
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2020
Anaerobic fungi from the herbivore digestive tract (
We report on the isolation of previously-uncultured Neocallimastigomycota SK4 lineage, by two independent research groups, from a wild aoudad sheep rumen sample (Texas, USA) and an alpaca fecal (Baden-Württemberg, Germany). Isolates both locations showed near-identical morphological microscopic features, forming medium-sized (2–5 mm) white filamentous colonies with center sporangia, agar roll tubes heavy biofilm in liquid media. Microscopic analysis revealed monocentric thalli, spherical...
Establishing a solid taxonomic framework is crucial for enabling discovery and documentation efforts. This ensures effective communication between scientists as well reproducibility of results laboratories, facilitates the exchange preservation biological material. Such can only be achieved by establishing clear criteria taxa characterization rank assignment. Within anaerobic fungi (phylum Neocallimastigomycota), need such especially vital. Difficulties associated with their isolation,...
We report on the isolation of previously uncultured Neocallimastigomycota SK4 lineage by two independent research groups from a wild aoudad sheep rumen sample (Texas, USA) and an alpaca fecal (Baden-Württemberg, Germany). Isolates both locations showed near identical morphological microscopic features, forming medium-sized white filamentous colonies with center sporangia agar roll tubes heavy biofilm in liquid media. Microscopic analysis revealed monocentric thalli, spherical...
Anaerobic fungi (AF), belonging to the phylum Neocallimastigomycota, are a pivotal component of digestive tract microbiome various herbivorous animals. In last decade, diversity AF has rapidly expanded due exploration numerous (novel) habitats. Studies aiming at understanding role require robust and reliable isolation cultivation techniques, many which remained unchanged for decades. Using amplicon sequencing, we compared three different media: medium with rumen fluid (RF), depleted (DRF),...
Anaerobic fungi (AF, phylum Neocallimastigomycota) are best known for their ability to anaerobically degrade recalcitrant lignocellulosic biomass through mechanic and enzymatic means. While biotechnological potential is well-recognized, applied research on AF still hampered by the time-consuming cost-intensive laboratory routines required isolate, maintain, preserve cultures. Reliable long-term preservation of specific strains would aid basic as well research, but commonly used protocols can...