A5047601661- Seismic Waves and Analysis
- Methane Hydrates and Related Phenomena
- Earthquake Detection and Analysis
- Seismology and Earthquake Studies
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Probiotics and Fermented Foods
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Anaerobic Digestion and Biogas Production
- Plant Virus Research Studies
- Bacterial Genetics and Biotechnology
- Enterobacteriaceae and Cronobacter Research
- Gene Regulatory Network Analysis
- Enzyme-mediated dye degradation
- Microplastics and Plastic Pollution
- Enzyme Catalysis and Immobilization
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Microbial Natural Products and Biosynthesis
- Enzyme Production and Characterization
- Microbial bioremediation and biosurfactants
- Fungal and yeast genetics research
University of Delaware
2020-2025
Purdue University West Lafayette
2017-2023
South University
2019-2023
State Street (United States)
2020-2023
University of California, Santa Barbara
2013-2018
Leibniz Institute of Plant Genetics and Crop Plant Research
2013
California Institute of Technology
2013
Beijing Institute of Genomics
2013
Heidelberg University
2013
University of Cambridge
2013
The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce large array biomass-degrading synergistically degrade crude, untreated plant biomass are competitive with optimized commercial preparations Aspergillus Trichoderma. Compared...
Anaerobic fungi (class Neocallimastigomycetes) thrive as low-abundance members of the herbivore digestive tract. The genomes anaerobic gut are poorly characterized and have not been extensively mined for biosynthetic enzymes natural products such antibiotics. Here, we investigate potential to synthesize that could regulate membership within microbiome. Complementary 'omics' approaches were combined catalog from four different representative species: Anaeromyces robustus (Arobustus),...
The conversion of lignocellulose-rich biomass to bio-based chemicals and higher order fuels remains a grand challenge, as single-microbe approaches often cannot drive both deconstruction chemical production steps. In contrast, consortia based bioprocessing leverages the strengths different microbes distribute metabolic loads achieve process synergy, product diversity, bolster yields. Here, we describe biphasic fermentation scheme that combines lignocellulolytic action anaerobic fungi...
Polyethylene (PE) is the most commonly used plastic on Earth due to its favorable material properties such as high ductility, mechanical strength, and bond homogeneity that make resistant deconstruction. However, lack of robust recycling infrastructure for PE end-of-life management leading an estimated 4 million tons environmental accumulation annually, with implications human health. Biological deconstruction upcycling could potentially aid in waste by allowing high-yield conversion...
The metabolism of archaeal methanogens drives methane release into the environment and is critical to understanding global carbon cycling. Methanogenesis operates at a very low reducing potential compared other forms respiration therefore many anaerobic environments. Harnessing or altering methanogen has mitigate warming even be utilized for energy applications.Here, we report draft genome sequences isolated Methanobacterium bryantii, Methanosarcina spelaei, Methanosphaera cuniculi,...
Prokaryotic Argonautes (pAgos) have been proposed as more flexible tools for gene-editing they do not require sequence motifs adjacent to their targets function, unlike popular CRISPR/Cas systems. One promising pAgo candidate, from the halophilic archaeon Natronobacterium gregoryi (NgAgo), has subject of debate regarding its potential in eukaryotic Here, we revisit this enzyme and characterize function prokaryotes. NgAgo expresses poorly non-halophilic hosts with most protein being insoluble...
Establishing a solid taxonomic framework is crucial for enabling discovery and documentation efforts. This ensures effective communication between scientists as well reproducibility of results laboratories, facilitates the exchange preservation biological material. Such can only be achieved by establishing clear criteria taxa characterization rank assignment. Within anaerobic fungi (phylum Neocallimastigomycota), need such especially vital. Difficulties associated with their isolation,...
Abstract Biocatalytic degradation of non-hydrolyzable plastics is a rapidly growing field research, driven by the global accumulation waste. Enzymes capable cleaving carbon-carbon bonds in synthetic polymers are highly sought-after as they may provide tools for environmentally friendly plastic recycling. Despite some reports oxidative enzymes acting on plastics, including polyethylene or poly(vinyl chloride), notion that these materials susceptible to efficient enzymatic remains...
Anaerobic fungi are among the most active plant‐degrading microbes in nature. Increased insight into mechanisms and environmental cues that regulate fungal hydrolysis would better inform bioprocessing strategies to depolymerize lignocellulose. Here, we compare response of three strains anaerobic ( Piromyces finnis , Anaeromyces robustus Neocallimastix californiae ) catabolite regulation by simple carbohydrates. exhibited high enzymatic activity against crystalline cellulose, which was...