A5034256236- Ionosphere and magnetosphere dynamics
- Geophysics and Gravity Measurements
- Earthquake Detection and Analysis
- Methane Hydrates and Related Phenomena
- Astrophysics and Cosmic Phenomena
- Seismic Waves and Analysis
- Solar and Space Plasma Dynamics
- Seismology and Earthquake Studies
- Superconducting Materials and Applications
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Geophysical Methods and Applications
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- Microbial Community Ecology and Physiology
- Galectins and Cancer Biology
- Neonatal Respiratory Health Research
- Medical Imaging and Pathology Studies
- Advanced Proteomics Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- Anaerobic Digestion and Biogas Production
- Effects of Radiation Exposure
- Sugarcane Cultivation and Processing
- Pancreatic and Hepatic Oncology Research
Pacific Northwest National Laboratory
2020-2025
Environmental Molecular Sciences Laboratory
2019-2024
University of Wisconsin–Madison
2024
Battelle
2023-2024
Abstract Successful establishment of pregnancy requires adhesion an embryo to the endometrium and subsequent invasion into maternal tissue. Abnormalities in this critical process implantation placentation lead many complications. Here we present a microenigneered system model complex sequence orchestrated multicellular events that plays essential role early pregnancy. Our implantation-on-a-chip is capable reconstructing three-dimensional structural organization maternal-fetal interface...
Abstract The rumen harbors a complex microbial mixture of archaea, bacteria, protozoa, and fungi that efficiently breakdown plant biomass its dietary carbohydrates into soluble sugars can be fermented subsequently converted metabolites nutrients utilized by the host animal. While bacterial populations have been well documented, only fraction eukarya are taxonomically functionally characterized, despite recognition they contribute to cellulolytic phenotype microbiota. To investigate how...
Anaerobic fungi (class Neocallimastigomycetes) thrive as low-abundance members of the herbivore digestive tract. The genomes anaerobic gut are poorly characterized and have not been extensively mined for biosynthetic enzymes natural products such antibiotics. Here, we investigate potential to synthesize that could regulate membership within microbiome. Complementary 'omics' approaches were combined catalog from four different representative species: Anaeromyces robustus (Arobustus),...
In many anoxic environments, syntrophic acetate oxidation (SAO) is a key pathway mediating the conversion of into methane through obligate cross-feeding interactions between SAO bacteria (SAOB) and methanogenic archaea. The particularly important in engineered environments such as anaerobic digestion (AD) systems operating at thermophilic temperatures and/or with high ammonia. Despite widespread importance SAOB to stability AD process, little known about their situ physiologies due typically...
Bronchopulmonary dysplasia (BPD) is a disease of prematurity related to the arrest normal lung development. The objective this study was better understand how proteome modulation and cell-type shifts are noted in BPD pathology. Pediatric human donors aged 1-3 yr were classified based on history histopathology consistent with "healed" (hBPD, n = 3) "established" (eBPD, compared respective full-term born (n 6) age-matched term controls. Proteins quantified by tandem mass spectroscopy selected...
Idiopathic pulmonary fibrosis (IPF) is a chronic disease that characterized by an excessive accumulation of extracellular matrix (ECM) proteins (e.g. collagens) in the parenchyma, which ultimately leads to respiratory failure and death. While current therapies exist slow progression, no are available resolve fibrosis. We O-linked N-Acetylglucosamine (O-GlcNAc) transferase (OGT)/O-GlcNAc axis IPF using single-cell RNA-sequencing (scRNA-seq) data human lung sections isolated fibroblasts from...
mecA -dependent methicillin resistance in MRSA is subject to regulation by numerous accessory factors involved cell wall biosynthesis, nucleotide signaling, and central metabolism. Here, we report that mutations the TCA cycle gene, sucC , increased susceptibility β-lactam antibiotics was accompanied significant accumulation of succinyl-CoA, which turn perturbed lysine succinylation proteome.
Viruses impact microbial systems through killing hosts, horizontal gene transfer, and altering cellular metabolism, consequently impacting nutrient cycles. A virus-infected cell, a "virocell," is distinct from its uninfected sister cell as the virus commandeers machinery to produce viruses rather than replicate cells. Problematically, virocell responses nutrient-limited conditions that abound in nature are poorly understood. Here we used biology approach investigate metabolic reprogramming...
Proteinaceous aggregates containing α-synuclein protein called Lewy bodies in the substantia nigra is a hallmark of Parkinson's disease. The molecular mechanisms body formation and associated neuronal loss remain largely unknown. To gain insights into proteins pathways with pathology, we performed quantitative profiling proteome. We analyzed tissue from 51 subjects arranged three groups: cases body-negative controls matching loss, no loss. Using label-free liquid chromatography-tandem mass...
Abstract Mass spectrometry imaging (MSI) is an invaluable tool for the spatial visualization of molecules in vivo. However, question whether observed annotations are endogenous or artificial (i. e., from in‐source fragmentation) critical and has been largely unexplored multimodal MSI. In matrix‐assisted laser desorption/ionization (MALDI)‐MSI datasets researchers worldwide, PAs were found to represent up 18 % rat brain. Rat brain was additionally imaged here using nanospray desorption...
Protein posttranslational modifications (PTMs) by O-GlcNAc globally rise during pressure-overload hypertrophy (POH). However, a major knowledge gap exists on the specific proteins undergoing changes in levels POH primarily because this PTM is low abundance and easily lost standard mass spectrometry (MS) conditions used for protein identification. Methodologies have emerged to enrich samples O-GlcNAcylated prior MS analysis. Accordingly, our goal was identify POH. We C57/Bl6 mice subjected...
Human disease states are biomolecularly multifaceted and can span across phenotypic states, therefore it is important to understand diseases on all levels, cell types, within microanatomical tissue compartments. To obtain an accurate representative view of the molecular landscape human lungs, this fragile must be inflated embedded maintain spatial fidelity location molecules minimize degradation for imaging experiments. Here, we evaluated agarose inflation carboxymethyl cellulose embedding...
E-cigarette liquids are complex mixtures of chemicals consisting humectants, such as propylene glycol (PG) and vegetable glycerin (VG), with nicotine or flavorings added. Published literature emphasizes the toxicity e-cigarette aerosols whereas much less attention has been given to biologic effects humectants. The purpose current study was provide a comprehensive view acute on rat bronchoalveolar lavage (BAL) using mass spectrometry-based global proteomics. Sprague-Dawley rats were exposed...
Abstract Background Sensitive screening for early Alzheimer’s disease (AD)‐related cognitive decline are needed. Prior research links high beta‐amyloid (Aβ) levels to reduced proper name (PN) retrieval in individuals without impairment. We examined whether language‐related regional tau from PET associated with Logical Memory (LM) recall, accounting LM covariates. Method Participants included n = 396 the Wisconsin Registry Prevention (WRAP) story amyloid ([11C]Pittsburgh Compound‐B, PiB), and...
Abstract Background Sensitive screening for early Alzheimer’s disease (AD)‐related cognitive decline are needed. Prior research links high beta‐amyloid (Aß) levels to reduced proper name (PN) retrieval in individuals without impairment. We examined whether language‐related regional tau from PET associated with Logical Memory (LM) recall, accounting LM covariates. Method Participants included n=396 the Wisconsin Registry Prevention (WRAP) story amyloid ([11C]Pittsburgh Compound‐B, PiB), and...