A5017794876- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Pregnancy and preeclampsia studies
- Prenatal Screening and Diagnostics
- Ovarian function and disorders
- Reproductive Health and Technologies
- Endometriosis Research and Treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ectopic Pregnancy Diagnosis and Management
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Pluripotent Stem Cells Research
- Uterine Myomas and Treatments
- Genetic Syndromes and Imprinting
- Ovarian cancer diagnosis and treatment
- Renal and related cancers
- Gynecological conditions and treatments
- Sperm and Testicular Function
- Sexual Differentiation and Disorders
- Immune Cell Function and Interaction
- COVID-19 Impact on Reproduction
- Pregnancy and Medication Impact
- Preterm Birth and Chorioamnionitis
- Diversity and Career in Medicine
University of Pennsylvania
2016-2025
Institute for Reproductive Health
2025
Hospital of the University of Pennsylvania
2008-2025
Philadelphia University
2024
University of Pennsylvania Health System
2016-2022
California University of Pennsylvania
2021
Penn Center for AIDS Research
2013-2017
California Institute for Regenerative Medicine
2016
Abstract Successful establishment of pregnancy requires adhesion an embryo to the endometrium and subsequent invasion into maternal tissue. Abnormalities in this critical process implantation placentation lead many complications. Here we present a microenigneered system model complex sequence orchestrated multicellular events that plays essential role early pregnancy. Our implantation-on-a-chip is capable reconstructing three-dimensional structural organization maternal-fetal interface...
Assisted reproductive technologies (ART) are associated with several complications including low birth weight, abnormal placentation and increased risk for rare imprinting disorders. Indeed, experimental studies demonstrate ART procedures independent of existing infertility induce epigenetic perturbations in the embryo extraembryonic tissues. To test hypothesis that these persist result adverse outcomes at term, we assessed placental morphology methylation profiles E18.5 mouse concepti...
Computer-automated time-lapse analysis has been shown to improve embryo selection by providing quantitative and objective information supplement traditional morphology. In this multi-centre study, the relationship between such computer-derived outputs (High, Medium, Low scores), implantation clinical pregnancy were examined. Data collected from six clinics, including 205 patients whose embryos imaged Eeva(TM) System. The Eeva scores blinded not considered during selection. Embryos with High...
We, and others, have demonstrated previously that there are differences in DNA methylation transcript levels of a number genes cord blood placenta between children conceived using assisted reproductive technologies (ART) vivo. The source these (the effect ART versus the underlying infertility) has never been determined humans. In this study, we attempted to resolve issue by comparing placental at 37 CpG sites 16 identified candidate independent populations vivo ('fertile control' group) with...
We analyzed placental DNA methylation levels at repeated sequences (LINE1 elements) and all CCGG sites (the LUMA assay) to study the effect of modifiable clinical or laboratory procedures involved in vitro fertilization. included four potential factors: oxygen tension during embryo culture, fresh transfer vs frozen transfer, intracytoplasmic sperm injection (ICSI) conventional insemination day 3 5 transfer. Global differed between placentas from natural conceptions compared conceived by IVF....
Definitive hematopoietic stem and progenitor cells (HSPCs) arise from a small number of hemogenic endothelial (HECs) within the developing embryo. Understanding origin ontogeny HSPCs is considerable interest potential therapeutic value. It has been proposed that murine placenta contains HECs differentiate into HSPCs. However, during human gestation in aorta considerably earlier than when they can first be detected placenta, suggesting may primarily serve as niche. We found Runx1...
Assisted reproductive technologies (ART) have been associated with several adverse perinatal outcomes involving placentation and fetal growth. It is critical to examine each intervention individually in order assess its relationship the described outcomes. One ubiquitously used ART superovulation gonadotropins. Superovulation results significant changes hormonal milieu, which persist during peri-implantation early periods. Epidemiologic evidence suggests that treatment-induced maternal...
High-grade serous carcinoma (HGSC) is the most lethal gynecological malignancy in United States. Late diagnosis and emergence of chemoresistance have prompted studies into how tumor microenvironment, more recently innervation, may be leveraged for HGSC prevention interception. In addition to stess-induced sources, concentrations sympathetic neurotransmitter norepinephrine (NE) ovary increase during ovulation after menopause. Importantly, NE exacerbates advanced progression. However, little...
Introduction Prior to pregnancy, hormonal changes lead cellular adaptations in the endometrium allowing for embryo implantation. Critical successful pregnancy establishment, innate immune cells constitute a significant proportion of uterine prior arrival and throughout first trimester humans animal models. Abnormal cell function during implantation is believed play role multiple adverse outcomes. Current work has focused on present after limited vitro models exist explore unique functions...
Epidemiological studies suggest that babies born following in vitro fertilization (IVF) and fresh embryo transfer are of lower birthweight than frozen transfer, although the mechanism responsible for this phenotype is not known. We developed a novel mouse model isolates independent effects freezing superovulated environment, which cannot be performed humans. transferred blastocysts had been vitrified warmed, mixed with blastocysts, into individual pseudopregnant recipients produced by either...
For successful embryo implantation, endometrial stromal cells must undergo functional and morphological changes, referred to as decidualization. However, the molecular mechanisms that regulate implantation decidualization are not well defined. Here we demonstrate estradiol- progesterone-regulated microRNA (miR)-200 family was markedly down-regulated in mouse prior whereas zinc finger E-box binding homeobox-1 -2 other known predicted targets were up-regulated. Conversely, miR-200 up-regulated...
How does an altered maternal hormonal environment, such as that seen during superovulation with gonadotropins in ART, impact human uterine immune cell distribution and function the window of implantation?
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection and resulting disease (COVID‐19) cause placental dysfunction, which increases the risk of adverse pregnancy outcomes. While abnormal pathology from COVID‐19 is common, direct placenta rare. This suggests that pathophysiology associated with maternal COVID‐19, rather than infection, responsible for dysfunction. We hypothesized circulating extracellular vesicles (EVs), altered by during pregnancy, contribute to To...
The oocyte-to-embryo transition entails genome activation and a dramatic reprogramming of gene expression that is required for continued development. Superimposed on development transcriptionally repressive state at the level chromatin structure. Inducing global histone hyperacetylation relieves this repression deacetylases 1 2 (HDAC1 HDAC2) are involved in establishing state. Because SIN3A an HDAC1/2-containing complex, we investigated whether it during course activation. We find Sin3a mRNA...
Abstract In vitro fertilization (IVF) is associated with DNA methylation abnormalities and a higher incidence of adverse pregnancy outcomes. However, which exposure(s), among the many IVF interventions, contributes to these outcomes remains unknown. Frozen embryo transfer (ET) increasingly utilized as an alternative fresh ET, but reports suggest pre-eclampsia large for gestational age infants. This study examines in human placentas using 850K Infinium MethylationEPIC BeadChip array obtained...