Polycaprolactone (PCL) is a polymer material suitable for being prepared into porous scaffolds used in bone tissue engineering, however, insufficient osteogenic ability and mechanical strength limit its application. Zinc (Zn) alloy with proper osteogenesis promising biodegradable metal that have attracted much attention. Herein, we combined the advantages of PCL Zn by fabricating PCL/Zn composite different powder contents (1 ​wt%, 2 3 ​wt%) through fused deposition modelling. The ​mechanical...

Currently, healing of large bone defects faces significant challenges such as a bulk regeneration and revascularization on the defect region. Here, "cell-free scaffold engineering" strategy that integrates strontium (Sr) highly bioactive serum exosomes (sEXOs) inside three-dimensional (3D)-printed titanium (Ti) (Sc) is first developed. The constructed SrTi Sc can serve sophisticated biomaterial platform for maintaining morphological characteristics radius during period critical (CBD) repair...

Abstract Mesenchymal stem cells (MSCs) are widely used in the treatment of diseases. After their vivo application, MSCs undergo apoptosis and release apoptotic vesicles (apoVs). This study investigates role apoVs derived from human bone marrow mesenchymal (hBMMSCs) metabolism molecular mechanism observed effects. The results show that can promote osteogenesis inhibit osteoclast formation vitro vivo. ApoVs may therefore attenuate loss caused by primary secondary osteoporosis stimulate...

D-mannose exhibits strong anti-inflammatory properties, but whether it has beneficial effects on preventing and treating osteoporosis remains unknown.Female, 12-month-old senile C57BL6/J mice (s-Man group) 8-week-old ovariectomized (OVX-Man were treated with in drinking water for 2 months (six mice/group). Microcomputed tomography analysis hematoxylin eosin staining performed to investigate the effect of attenuation bone loss. Tartrate-resistant acid phosphatase tissue sections, flow...

Guided bone regeneration membranes have been effectively applied in oral implantology to repair defects. However, typical resorbable composed of collagen (Col) insufficient mechanical properties and high degradation rate, while non-resorbable need secondary surgery. Herein, we designed a photocrosslinkable collagen/polycaprolactone methacryloyl/magnesium (Col/PCLMA/Mg) composite membrane that provided spatiotemporal support effect after photocrosslinking. Magnesium particles were added the...

Background: Although antiretroviral agents trigger bone loss in human immunodeficiency virus patients, tenofovir disoproxil fumarate (TDF) induces more severe damage, such as osteoporosis. While, the mechanisms are unclear, probiotic supplements may be effective against Methods: C57BL6/J mice were administered with Lactobacillus rhamnosus GG (LGG)+TDF, TDF, and zoledronic acid+TDF, respectively. Bone morphometry biomechanics evaluated using microcomputed tomography, slicing, flexural tests....

Abstract Background In tissue engineering, mesenchymal stem cells (MSCs) are common seed because of abundant sources, strong proliferation ability and immunomodulatory function. Numerous researches have demonstrated that MSC-macrophage crosstalk played a key role in the engineering. Macrophages could regulate differentiation MSCs via different molecular mechanisms, including extracellular vesicles. Apoptotic macrophages generate large amounts apoptotic vesicles (apoVs). ApoVs rich proteins,...

Abstract Background Astronauts undergo significant microgravity-induced bone loss during space missions, which has become one of the three major medical problems hindering human's long-term flight. A risk-free and antiresorptive drug is urgently needed to prevent missions. D-mannose a natural C-2 epimer D-glucose abundant in cranberries. This study aimed investigate protective effects potential mechanisms against under weightlessness. Methods The hind legs tail-suspended (TS) rats were used...

Bone defects are a common clinical condition that has gained an increasing amount of attention in recent years. Causes bone defect include tumors, inflammation, and fractures. tissue engineering is novel treatment defect, human mesenchymal stem cells (hMSCs) the ideal seed for due to their multi-lineage differentiation potential immunogenicity. The laminin α2 (LAMA2) gene encodes subunit laminins. Mutations this have been reported cause muscular dystrophy, but thus far no studies elucidated...

In bone tissue engineering, polycaprolactone (PCL) is a promising material with good biocompatibility, but its poor degradation rate, mechanical strength, and osteogenic properties limit application. this study, we developed an Mg-1Ca/polycaprolactone (Mg-1Ca/PCL) composite scaffolds to overcome these limitations. We used melt blending method prepare Mg-1Ca/PCL composites Mg-1Ca alloy powder mass ratios of 5, 10, 20 wt%. Porous controlled macro- microstructure were printed using the fused...

Zirconia as a promising dental implant material has attracted much attention in recent years. Improving the bone binding ability of zirconia is critical for clinical applications. Here, we established distinct micro-/nano-structured porous through dry-pressing with addition pore-forming agents followed by hydrofluoric acid etching (POROHF). Porous without treatment (PORO), sandblasting plus acid-etching zirconia, and sintering surface were applied controls. After human marrow mesenchymal...

Poly-ε-caprolactone (PCL) is a promising synthetic material in bone tissue engineering (BTE). Particularly, the introduction of rapid prototyping (RP) represents possibility manufacturing PCL scaffolds with customized appearances and structures. Bio-Oss natural mineral matrix significant osteogenic effects; however, it has limitations being constructed maintained into specific shapes sites. In this study, we used RP fabricated hollow-structured cage-shaped scaffold loaded to form hybrid for...

As the representative of fenamic acids, an important group NSAIDs, flufenamic acid (FFA) has been used for anti-inflammation and analgesia in clinic. Recently, researches have focused on role some members NSAIDs promoting osteogenesis. However, little attention paid to subgroup it remains unclear whether FFA other acids could regulate mesenchymal stem cells' (MSCs) lineage commitment bone regeneration. Here we treated two kinds human MSCs with at different concentrations vitro examined...

Objectives . Flufenamic acid (FFA) is a representative of the fenamic acids, an important group NSAIDs. In present study, we study effects FFA on adipogenesis human mesenchymal stem cells (MSCs) and explore potential mechanism. Methods To investigate adipogenic differentiation hMSCs, adipose-derived (hASCs) bone marrow (hBMMSCs), were treated with during in vitro The vivo evaluated using heterotopic adipose formation assay nude mice as well ovariectomized (OVX) aged mice. mechanism FFA,...

Abstract Background Tenofovir disoproxil fumarate (TDF), a primary antiretroviral agent used to treat AIDS, triggers systematic bone loss. However, the effect of TDF on osteopenia or osteoporosis in jaw remains unclear. TDF‐induced loss jaw, if any, likely involves mandible‐derived mesenchymal stem cells (MMSCs), which play key role jawbone metabolism. Probiotics prevent long loss, and could prove efficacious treating mandibular Objectives To determine whether elucidate underlying...

Cell-Free Therapies hBMMSC-apoVs can promote mesenchymal stem cell (MSC) osteogenesis and inhibit osteoclast formation in vitro vivo. The mechanisms include the release of miR1324, which inhibits expression target gene SNX14, thereby activating SMAD1/5 pathway cells. In article number 2205813, Xiao Zhang, Yunsong Liu, co-workers suggest a therapeutic strategy for treatment bone loss, including tissue engineering through cell-free approach.

Abstract Background The effect of platelet factor 4 (PF4) on bone marrow mesenchymal stem cells (BMMSCs) and osteoporosis is poorly understood. Therefore, this study aimed to evaluate the effects PF4‐triggered destruction in mice determine underlying mechanism. Methods First, vitro cell proliferation cycle BMMSCs were assessed using a CCK8 assay flow cytometry, respectively. Osteogenic differentiation was confirmed staining quantification alkaline phosphatase Alizarin Red S. Next, an...

Both estrogen deficiency and aging may lead to osteoporosis. Developing novel drugs for treating osteoporosis is a popular research direction. We screened several potential therapeutic agents through new deep learning-based efficacy prediction system (DLEPS) using transcriptional profiles DLEPS screening led drug examinee, ataluren, Ataluren significantly reversed bone loss in ovariectomized mice. Next, ataluren increased human marrow-derived mesenchymal stem cell (hBMMSC) osteogenic...

Abstract Background Osteoporosis is a chronic bone disease characterized by loss and decreased strength. However, current anti‐resorptive drugs carry risk of various complications. The deep learning‐based efficacy prediction system (DLEPS) forecasting tool that can effectively compete in drug screening based on gene expression changes. This study aimed to explore the protective effect potential mechanisms cinobufotalin (CB), traditional Chinese medicine (TCM), loss. Methods DLEPS was...

Abstract BackgroundBoth estrogen deficiency and aging may lead to osteoporosis. Developing novel drugs for treating osteoporosis is a popular research direction. Ataluren (ATA) nonaminoglycoside nonsense mutation inhibitor currently used clinically treat muscular atrophy Duchenne dystrophy. However, little attention has been given the role of in osteogenesis, it unclear whether can improve osteogenic differentiation human- derived bone marrow mesenchymal stem cells (hBMMSCs)...