A5023531962- Fungal and yeast genetics research
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Biofuel production and bioconversion
- Nematode management and characterization studies
- Fungal Biology and Applications
- Polysaccharides and Plant Cell Walls
- Microbial Inactivation Methods
- Microbial Metabolic Engineering and Bioproduction
- Essential Oils and Antimicrobial Activity
- Microbial Metabolites in Food Biotechnology
- scientometrics and bibliometrics research
- Academic Publishing and Open Access
- Enzyme Production and Characterization
- Bee Products Chemical Analysis
- Complex Systems and Decision Making
- University-Industry-Government Innovation Models
- Herbal Medicine Research Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Oral Health Pathology and Treatment
- Genetics, Aging, and Longevity in Model Organisms
- Oral and Maxillofacial Pathology
- Enzyme-mediated dye degradation
- Probiotics and Fermented Foods
- Legume Nitrogen Fixing Symbiosis
Universidad de Murcia
2016-2025
Instituto Murciano de Investigación Biosanitaria
2016-2017
Ciência Viva
2015
University of Havana
1974-2008
Hospital Vilardebó
2006
KU Leuven
1990-1994
ABSTRACT Amphotericin B (AMB) is an antifungal drug that binds to ergosterol and forms pores at the cell membrane, causing loss of ions. In addition, AMB induces accumulation reactive oxygen species (ROS), although these molecules have multiple deleterious effects on fungal cells, their specific role in action mechanism remains unknown. this work, we studied ROS AMB. We determined intracellular induction 44 isolates different pathogenic yeast ( Candida albicans , parapsilosis glabrata...
The cellular response to the oxidative stress caused by hydrogen peroxide and its putative correlation with protector trehalose was investigated in Candida albicans CAI.4 tps1/tps1 double mutant, which is deficient synthesis. When exponential wild-type blastoconidia were exposed high concentrations of peroxide, they displayed a cell survival, accompanied marked rise intracellular trehalose. latter due moderate activation synthase concomitant inactivation neutral trehalase. Identical...
Addition of glucose or related fermentable sugars to derepressed cells the yeast Saccharomyces cerevisiae triggers a RAS-mediated cyclic AMP (cAMP) signal that induces protein phosphorylation cascade. In mutants (tpk1w1, tpk2w1, and tpk3w1) containing reduced activity cAMP-dependent kinase, sugars, as opposed nonfermentable carbon sources, induced permanent hyperaccumulation cAMP. This finding confirms previous conclusions are specific stimulators cAMP synthesis in cells. Despite huge levels...
Fungi are eukaryotic organisms with relatively few pathogenic members dangerous for humans, usually acting as opportunistic infections. In the last decades, several life-threatening fungal infections have risen mostly associated worldwide extension of chronic diseases and immunosuppression. The available antifungal therapies cannot combat this challenge because arsenal compounds is scarce displays low selective action, significant adverse effects, increasing resistance. A growing isolation...
Abstract In Candida albicans , trehalose plays an essential role as a protector of cell integrity against oxidative challenge. A double homozygous mutant, tps1/tps1 deficient in synthesis, displayed severe mortality when exposed to high H 2 O concentrations, compared with its congenic parental (CAI‐4) strain (Alvarez‐Peral et al. 2002 ). We have examined the putative set well‐known antioxidant enzymes components defence mechanism challenges. When mild non‐lethal treatment (0.5 m M ),...
Addition of glucose or related fermentable sugars to derepressed cells the yeast Saccharomyces cerevisiae triggers a RAS-mediated cyclic AMP (cAMP) signal that induces protein phosphorylation cascade. In mutants (tpk1w1, tpk2w1, and tpk3w1) containing reduced activity cAMP-dependent kinase, sugars, as opposed nonfermentable carbon sources, induced permanent hyperaccumulation cAMP. This finding confirms previous conclusions are specific stimulators cAMP synthesis in cells. Despite huge levels...
Abstract Since its discovery in the middle of XX century, research into autophagy has undergone a spectacular expansion, particularly early 1990s. A number physiological processes involving have been revealed and important human pathologies associated with perturbations autophagy. In 2008 “Guidelines for use interpretation assays monitoring autophagy” was launched purpose collecting single document all available information to monitor autophagy, which, it thought, might be useful established...
Candida albicans yeast cells growing exponentially on glucose are extremely sensitive to severe heat shock treatments (52.5°C for 5 min). When these cultures were subjected a mild temperature preincubation (42°C), they became thermotolerant and displayed higher resistance further stress. The intracellular content of trehalose was very low in exponential cells, but underwent marked increase upon non-lethal exposure. accumulation is likely due heat-induced activation the trehalose-6-phosphate...
Abstract Addition of a nitrogen‐source to glucose‐repressed, nitrogen‐starved G0 cells the yeast Saccharomyces cerevisiae in presence fermentable carbon source induces growth and causes within few minutes five‐fold, protein‐synthesis‐independent increase activity trehalase. Nitrogen‐activated trehalase could be deactivated vitro by alkaline phosphatase treatment, supporting idea that activation is triggered phosphorylation. Yeast strains containing only one three TPK genes (which encode...
After screening a Candida albicans genome data base, the product of an open reading frame (IPF 19760/CA2574) with 41% identity to Saccharomyces cerevisiae vacuolar acid trehalase (Ath1p) was identified and named Atc1p. The deduced amino sequence shows that Atc1p contains N-terminal hydrophobic signal peptide 20 potential sites for N-glycosylation. C. homozygous mutants lack activity were constructed by gene disruption at two ATC chromosomal alleles. Analysis these null is localized in cell...
In the pathogenic yeast Candida albicans, MAP-kinase Hog1 mediates an essential protective role against oxidative stress, a feature shared with transcription factor Cap1. We analysed adaptive response of strains both elements altered. Pretreatment gentle doses oxidants or thermal upshifts (28-->37 and 37-->42 degrees C) improved survival in face high concentrations (50 mM H(2)O(2) 40 menadione), pointing to functional cross-protective mechanism mutants. The challenge promoted marked...
Fungi have traditionally been considered opportunistic pathogens in primary infections caused by virulent bacteria, protozoan, or viruses. Consequently, antimycotic chemotherapy is clearly less developed comparison to its bacterial counterpart. Currently, the three main families of antifungals (polyenes, echinocandins, and azoles) are not sufficient control enormous increase life-threatening fungal recorded recent decades. Natural substances harvested from plants utilized as a successful...