A5055716507- Photodynamic Therapy Research Studies
- Mass Spectrometry Techniques and Applications
- Nanoplatforms for cancer theranostics
- Analytical Chemistry and Chromatography
- Ion-surface interactions and analysis
- Laser Applications in Dentistry and Medicine
- Photoacoustic and Ultrasonic Imaging
- Laser Material Processing Techniques
- Laser Design and Applications
- Gastric Cancer Management and Outcomes
- Laser-induced spectroscopy and plasma
- Metastasis and carcinoma case studies
- Porphyrin and Phthalocyanine Chemistry
- Optical Imaging and Spectroscopy Techniques
- Dermatologic Treatments and Research
- Laser-Matter Interactions and Applications
- Dental materials and restorations
- Esophageal Cancer Research and Treatment
- Dental Erosion and Treatment
- Skin Protection and Aging
- Coronary Interventions and Diagnostics
- Laser-Plasma Interactions and Diagnostics
- Advanced Proteomics Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Ocular and Laser Science Research
Osaka University
2015-2024
Japan Science and Technology Agency
2008-2012
Tokyo University of Science
1997-2002
The University of Tokyo
2001
University of Electro-Communications
1998
Significance: In laser therapy and diagnosis of skin diseases, the irradiated light distribution, which is determined by absorption coefficient μa reduced scattering μs′ epidermis, dermis, subcutaneous fat, affects treatment outcome accuracy. Although values for have been reported, detailed analysis Asian tissues still lacking. Aim: We present measurements in 400- to 1100-nm wavelength range evaluating optical penetration depth energy deposition. Approach: The with human samples are...
Diffuse reflectance spectroscopy is a widely used technique for medical applications that may analyze the optical characteristics of biological tissues. By using diffuse spectroscopy, different tissue types can be distinguished based on specific changes reflected light spectrum are result differences molecular level between compared Identification structural features performed by applying and spectra obtained from this could provide important diagnostic information about morphology...
Herein, the optical adequacy of a tumor model prepared with cells grown on chorioallantoic membrane (CAM) chicken egg is evaluated as an alternative to mouse assess optimal irradiation conditions in photodynamic therapy (PDT). The properties CAM and tissues were measured double integrating sphere inverse Monte Carlo technique 350- 1000-nm wavelength range. hemoglobin water absorption bands observed tissue (10 eggs 10 tumors) are equal that (8 animals 8 tumors). intersubject variability meets...
Computational clinical trial (CCT) in the field of laser medicine promotes application novel devices, because this carried out based on numerical modeling laser-tissue interactions and simulation a series treatment process. To confirm feasibility computational skin with picosecond laser, paper presents an evaluation method safety.In method, light propagation thermal diffusion process after ultrashort pulse irradiation to model is calculated safety photothermal damage evaluated by simulation....
Background and Objectives In this study, the threshold fluences for disrupting melanosomes pigmented skin lesion treatment were determined using a 755‐nm picosecond laser clinical use. Based on melanosome disruption thresholds, incident corresponding to target depths evaluated in silico different spot sizes. Study Design/Materials Methods Melanosome samples isolated from porcine eyes as alternative human cutaneous melanosomes. The exposed pulses measure mean particle sizes by dynamic light...
Mass spectrometry using matrix-assisted laser desorption/ionization (MALDI) technique is one of the most widely used method to analyze proteins in biological research fields. However, it difficult insoluble which have important roles researches on disease mechanisms or developments drugs by ultraviolet (UV) lasers commonly been for MALDI. Recently, a significant improvement MALDI process combination UV nitrogen and tunable mid-infrared (MIR) free electron (FEL) was reported. Since FEL very...
We have developed a new ion extraction method for stigmatic matrix-assisted laser desorption/ionization (MALDI) imaging time-of-flight (TOF) mass spectrometer.The conventional delayed is unsuitable TOF spectrometer because the ions disperse until and distribution not preserved imaging.In proposed method, temporal spatial focusing can be simultaneously achieved, enabling high-fidelity image to obtained with high resolution.This expected applicable cellular-level observations by spectrometry.
SignificancePhotobleaching of the photosensitizer reduces fluorescence observation time and intensity emitted for tumor detection during 5-aminolevulinic acid-based photodynamic diagnosis.AimThis study aims to utilize concept photoswitching, which uses emission from excitation followed by simultaneous its photoproduct increase PDD deeply located tumors.ApproachThe photobleaching protoporphyrin IX (PpIX) formation photoproduct, photoprotoporhyrin (Ppp), caused exposure 505 nm light were...
Interstitial photodynamic therapy (iPDT) with 5-aminolevulinic acid (ALA) is a possible alternative treatment for malignant brain tumors. Further evaluation is, however, required before it can be clinically applied. Computational simulation of the photophysical process in ALA-iPDT offer quantitative tool understanding outcomes, which depend on various variables related to clinical conditions. We propose method tumors using singlet oxygen (O12) model and O12 threshold induce cell death. In...
A novel stigmatic mass microscope using laser desorption/ionization and a multi-turn time-of-flight spectrometer, MULTUM-IMG, has been developed. Stigmatic ion images of crystal violet masked by fine square mesh grid with 12.7 μm pitch as well microdot patterns 5 dot diameter 10 made rhodamine B were clearly observed. The estimated spatial resolution was about 3 in the linear mode 20-fold optical magnification. Separating according to time-of-flight, i.e., mass-to-charge ratio ions...
Significance: The diagnostic depth of photodynamic diagnosis (PDD) for gastric cancer with protoporphyrin IX (PpIX) is limited, which leads to missing intramucosal cancers in screening and surgery. Aim: reason that the excitation light, whose wavelength determined by highest absorption peak PpIX (∼405 nm), strongly attenuated mucosal tissues. We investigated an can extend fluorescence at subsurface. Approach: By calculating depth-dependent intensity light porcine mucosa each wavelength,...
Laser vaporization of the prostate is considered to be a promising treatment for benign prostatic hyperplasia (BPH), and efficiency hemostasis are both important parameters such treatment. In this study, we used high-power laser diode with wavelength 980 nm obtain high good hemostasis. The objective study evaluate efficacy BPH in ex vivo experiments using 300-W quantitatively.An experimental setup simulating clinical situation was constructed. Bovine tissue as sample. power setting 100, 150,...
Mass spectrometry (MS) is a valuable tool that enables label-free analysis and the ability to measure multiple molecules. The atmospheric pressure MS imaging (MSI) method usually requires tedious sample preparation. A simple ionization with minimal preparation needed for high-throughput analysis. We have developed an ion source does not require such as thinning, curing, planarization, or addition of matrix by electrospray-assisted laser desorption/ionization gas transportation (ELDI-GT)....
Since resonant absorption of light caused by a variety different molecular bond occurs in the mid-infrared (MIR) wavelength region, many applications using tunable MIR lasers have been reported. However, applicable fields restricted their large size and high cost equipments. Therefore, we are developing compact laser an optical parametric oscillator (OPO). To obtain long term stability conversion efficiency, diode-pumped Q-switched Tm,Ho:YAG ceramic with 2.1 μm was adopted for pump source. A...
Photodynamic therapy (PDT), a minimally invasive cancer treatment involving the activation of photosensitizer by specific wavelength light, is considered to be promising option for drug-resistant prostate cancer. Hemagglutinating virus Japan envelope (HVJ-E) has potential serve as highly effective through selective drug delivery and enhancement anti-tumor immune response.