A5042399068- Vector-borne infectious diseases
- Insect and Pesticide Research
- Viral Infections and Vectors
- Bee Products Chemical Analysis
- Study of Mite Species
- Ubiquitin and proteasome pathways
- Entomological Studies and Ecology
- Protease and Inhibitor Mechanisms
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Angiogenesis and VEGF in Cancer
- Endoplasmic Reticulum Stress and Disease
- Mosquito-borne diseases and control
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- Insect Pest Control Strategies
- Invertebrate Immune Response Mechanisms
- Platelet Disorders and Treatments
- Glutathione Transferases and Polymorphisms
- Maternal and Neonatal Healthcare
- Biological Research and Disease Studies
- Crustacean biology and ecology
- Hemophilia Treatment and Research
- Extracellular vesicles in disease
- Yersinia bacterium, plague, ectoparasites research
Instituto Butantan
2016-2025
Universidade Federal de São Paulo
2012
The aim of this study was to evaluate the anti-tumor activity Amblyomin-X, a serine protease Kunitz-type inhibitor. Amblyomin-X induced tumor mass regression and decreased number metastatic events in B16F10 murine melanoma model. Alterations on expression several genes related cell cycle were observed when two lines treated with Amblyomin-X. PSMB2, which encodes proteasome subunit, differentially expressed, agreement inhibition proteasomal both lines. In conclusion, our results indicate that...
The coxal fluid of the tick species Ornithodoros brasiliensis (O. brasiliensis) was subjected to detailed chemical analysis using Instrumental Neutron Activation Analysis (INAA) and Energy Dispersive X-ray Fluorescence (EDXRF). These complementary techniques allowed identification quantification key elements such as sodium (Na), chlorine (Cl), potassium (K), zinc (Zn), along with others like sulfur (S), phosphorus (P), iron (Fe). study demonstrated consistency between two analytical methods...
In cancer-treatment, potentially therapeutic drugs trigger their effects through apoptotic mechanisms. Generally, cell response is manifested by Bcl-2 family protein regulation, the impairment of mitochondrial functions, and ROS production. Notwithstanding, several operate proteasome inhibition, which, inducing accumulation aggregation misfolded or unfolded proteins, can lead to endoplasmic reticulum (ER) stress. Accordingly, it was shown that Amblyomin-X, a Kunitz-type inhibitor identified...
Amblyomin-X is a Kunitz-type serine protease inhibitor (Kunitz-type SPI) designed from the cDNA library of Amblyomma cajennense tick, which displays in vivo anti-tumor activities. Here, mechanisms actions vascular endothelial growth factor A (VEGF-A)-induced angiogenesis were characterized. Topical application (10 or 100 ng/10 μl; each 48 h) inhibited VEGF-A-induced dorsal subcutaneous tissue male Swiss mice. Moreover, similar effect was observed chicken chorioallantoic membrane (CAM)....
Abstract Ornithodoros mimon is an argasid tick that parasitizes bats, birds and opossums also harmful to humans. Knowledge of the transcripts present in gut helps understanding role vital molecules digestion process parasite-host relationship, while providing information about evolution arthropod hematophagy. Thus, study aimed know ascertain main expressed after their blood meal, through analysis on transcriptome engorged females O. using 454-based RNA sequencing. The reveals several...
Specific blood coagulation inhibitors from hematophagous organisms, with different structures and novel mechanism of action, have been described they represent promising agents for the treatment a variety human diseases related to cancer. In our lab, salivary glands transcriptome adult Amblyomma cajennense tick was previously characterized by expressed sequence tags (EST). A transcript that codes tissue factor pathway inhibitor (TFPI)-like protein unique structure found, recombinant form...
<i>Background/Objective:</i> Renal ischemia-hypoxia is a leading cause of acute kidney injury (AKI). Ischemia causes extracellular matrix breakdown the tubular basement membrane. Endostatin (ES) C-terminal fragment collagen XVIII generated by proteolytic cleavage. Recent studies have demonstrated that ES expression upregulated in ischemic kidneys. The present study aimed to characterize from <i>Methods:</i> Ischemic renal failure was induced via 45 min occlusion left...
Neovascularization, a process that includes vasculogenesis and angiogenesis, may be physiological or pathologic event, but in any cases the phenomenon is related to formation of vascular net sprouting endothelial cells from preexisting blood vessel. The tumor environment, which counts on cell proliferation, plenty proangiogenic factors, such as angiogenin, TGF (α β), FGF, VEGF, all them playing crucial role an important hallmark cancer frequently poor prognosis. Therefore, therapies focusing...
Brazil is one of the largest suppliers bee products in world. It currently 5th exporter honey and derivatives a significant part its production originates from south northeast. Specifically, species Scaptotrigona aff. postica, region Barra do Corda State Maranhão (MA), produces pollen which main activities local economy (food supplement). However, there are no data on mineral composition, although history applications nutritional field. In this context, research aims to generate integrated...
Anaplasma marginale is a Gram-negative, obligate intraerythrocytic bacterium that causes bovine anaplasmosis. While hard ticks of the genera Dermacentor and Rhipicephalus can be biological vectors, transmitting this pathogen via saliva during blood meals, blood-sucking insects, fomites play role as mechanical vectors. Little known about interaction between Argasidae ticks. Among soft ticks, Ornithodoros fonsecai (Labruna Venzal) brasiliensis Aragão inhabit environments surrounding localities...
Ornithodoros fonsecai is a species of argasid tick endemic to Brazil, described in the “São Miguel” cave located municipality Bonito, state Mato Grosso do Sul, central-western region Brazil. The artificial feeding technique makes it possible study biology hematophagous arthropods using or natural membranes, as well different types blood and anticoagulants. Thus, aim present was feed artificially O. second instar (N2) nymphs with rabbit parafilm membrane anticoagulant Alsever. Ninety percent...
Oviposition and eclosion periods for Ixodes didelphidis were observed under two temperatures (25ºC 27ºC) 90-95% humidity. Although there was a significant increase in the period (p<0.05) tendency to oviposition at 25ºC, neither differences interval (days), until maximum peak of nor number emerging larvae during total emerged larvae. These temperature values are not critical embryological development species. Because 27ºC high humidity shorter, percentage is higher, we consider this be ideal...
In this study, multielemental analysis of Lonomia obliqua (Lepidoptera, Saturniidae) caterpillar was performed using energy dispersive X‐ray fluorescence and instrumental neutron activation techniques. This is poisonous has the ability to cause fatal hemorrhagic effects in humans after contact. The need study related morphological changes (mainly size color) observed some caterpillars used for preparation antilonomic serum (antivenom). samples were classified as healthy (caterpillars...