A5002769042- Enzyme Structure and Function
- Protein Structure and Dynamics
- Advanced X-ray Imaging Techniques
- Biochemical and Molecular Research
- X-ray Spectroscopy and Fluorescence Analysis
- Mass Spectrometry Techniques and Applications
- RNA and protein synthesis mechanisms
- Enzyme Production and Characterization
- Protein purification and stability
- Crystallization and Solubility Studies
- Photosynthetic Processes and Mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- RNA modifications and cancer
- Machine Learning in Bioinformatics
- Bacteriophages and microbial interactions
- Particle Accelerators and Free-Electron Lasers
- Microbial Metabolic Engineering and Bioproduction
- Microbial metabolism and enzyme function
- Cancer Research and Treatments
- X-ray Diffraction in Crystallography
- Supramolecular Self-Assembly in Materials
- Molecular Biology Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Porphyrin Metabolism and Disorders
- Amino Acid Enzymes and Metabolism
Brookhaven National Laboratory
2008-2022
Brookhaven College
2017-2021
RIKEN BNL Research Center
2017
SPring-8
2008
Seoul National University
1993
George Mason University
1987
University of California, Los Angeles
1974-1983
MRC Laboratory of Molecular Biology
1974
Medical Research Council
1974
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCrystal structure of the complex porcine trypsin with soybean inhibitor (Kunitz) at 2.6 Å resolutionR. M. Sweet, H. T. Wright, J. Janin, C. Chothia, and D. BlowCite this: Biochemistry 1974, 13, 20, 4212–4228Publication Date (Print):September 1, 1974Publication History Published online1 May 2002Published inissue 1 September 1974https://pubs.acs.org/doi/10.1021/bi00717a024https://doi.org/10.1021/bi00717a024research-articleACS PublicationsRequest...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular architecture of the cephalosporins. Insights into biological activity based on structural investigationsRobert M. Sweet and Lawrence F. DahlCite this: J. Am. Chem. Soc. 1970, 92, 18, 5489–5507Publication Date (Print):September 1, 1970Publication History Published online1 May 2002Published inissue 1 September 1970https://pubs.acs.org/doi/10.1021/ja00721a032https://doi.org/10.1021/ja00721a032research-articleACS PublicationsRequest reuse...
Site-directed mutagenesis and Laue diffraction data to 2.5 Å resolution were used solve the structures of two sequential intermediates formed during catalytic actions isocitrate dehydrogenase. Both are distinct from enzyme-substrate enzyme-product complexes. Mutation key residues changed rate determining steps so that protein substrate within overall reaction pathway could be visualized.
Crystals of bovine trypsin were acylated at the reactive residue, serine 195, to form transiently stable p-guanidinobenzoate. Hydrolysis this species was triggered in crystals by a jump pH. The hydrolysis monitored three-dimensional Laue crystallography, resulting three x-ray diffraction structures, all from same crystal and each representing approximately 5 seconds exposure. structures analyzed nominal resolution 1.8 angstroms sufficient quality reproduce subtle features electron-density...
Two new macromolecular crystallography (MX) beamlines at the National Synchrotron Light Source II, FMX and AMX, opened for general user operation in February 2017 [Schneider et al. (2013). J. Phys. Conf. Ser. 425 , 012003; Fuchs (2014). 493 012021; (2016). AIP Proc. SRI2015 1741 030006]. FMX, micro-focusing Frontier MX beamline sector 17-ID-2 NSLS-II, covers a 5–30 keV photon energy range delivers flux of 4.0 × 10 12 photons s −1 1 Å into µm 1.5 to (V H) variable focus, expected reach 5...
Crystals of the protein B-phycoerythrin from red alga Porphyridium cruentum undergo twinning by merohedry in which two members twin are related rotation about (1,1,-1,0), a symmetry operation lattice but not crystals. Several methods compared for estimation volume fractions so that measured data can be corrected this twinning. The effect these corrections on final electron-density map is analyzed. Results show when fraction smaller crystal twinned specimen used structure determination...
Crystals of E. coli cytochrome b 1, alias bacterioferritin, were grown from a low ionic strength solution. The resulting monoclinic P 2 1 structure was solved by molecular replacement and refined using noncrystallographic symmetries applied to the fundamental unit, consisting two protein subunits single haem. From Patterson self-rotation results it shown that asymmetric unit crystal consists 12 such dimers corresponds complete, nearly spherical, molecule bacterioferritin ( M r = 450 kDa) 432...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure of an acyl-enzyme intermediate during catalysis: (guanidinobenzoyl)trypsinWalter F. Mangel, Paul T. Singer, Donna M. Cyr, Timothy C. Umland, Diana L. Toledo, Robert Stroud, James W. Pflugrath, and SweetCite this: Biochemistry 1990, 29, 36, 8351–8357Publication Date (Print):September 11, 1990Publication History Published online1 May 2002Published inissue 11 September...
Single crystal x-ray diffraction studies are proceeding on two phycobiliproteins: C-Phycocyanin from Anabaena variabilis and B-Phycoerythrin Porphyridium cruenturn consists of six a fl subunits.A three-dimensional dieaction study this light-harvesting protein (P63; = b 154.1 A, c 40.1 A) at 6-A resolution shows that the molecule is 110 A in diameter, 40 thick, with 20-A diameter central channel.It can be divided into three separate domains related by crystallographic 3 contains long,...
We describe a high throughput method for screening up to 1728 distinct chemicals with protein crystals on single microplate. Acoustic droplet ejection (ADE) was used co-position 2.5nL of protein, precipitant, and MiTeGen in situ-1 crystallization plate™ by co-crystallization or soaking. ADE-transferred droplets follow precise trajectory which allows all components be transferred through small apertures the microplate lid. The were large enough pass them, but so that they did not disrupt...
Abstract Short segments of polypeptide, from a protein for which the primary sequence but not three‐dimensional structure is known, are compared to library known structures. The basis comparison probability with residues in unknown segment might substitute through evolution structure. In test cases, structures that similar those treated as often found be one another and true “unknown” segment. This provides prediction local configuration (secondary structure) polypeptides.
Nostoc sp. strain MAC cyanobacteria were green in color when grown white light at 30 degrees C and contained phycobilisomes that had phycoerythrin phycocyanin a molar ratio of 1:1. Cells for 4 to 5 days or 39 turned brown greater than 2:1. In addition the change pigment composition, from cells missing 34.5-kilodalton, rod-associated peptide was present cells. The light-induced changes typical chromatic adaptation response cyanobacteria, but induction similar by growth new observation....
Here we present two robotic sample changers integrated into the experimental stations for macromolecular crystallography (MX) beamlines AMX and FMX, biological small-angle scattering (bioSAXS) beamline LiX. They enable fully automated unattended data collection remote access to beamlines. The system designs incorporate high-throughput, versatility, high-capacity, resource sharing robustness. All systems are centered around a six-axis industrial arm coupled with force torque sensor in-house...
An Introduction to the Symposium.- Symposium Lecture.- Developments in X-ray Technology and Their Contribution Structural Biology.- Some of Synchrotron Facilities for Biological Studies.- MacChess - A Macromolecular Diffraction Resource at Cornell High Energy Source.- Available Biophysics Research Stanford Radiation Laboratory.- Use Institute Solid State Physics Photon Factory.- Studies LURE Facility.- Beamlines European Molecular Biology Laboratory Outstation In Hamburg.- Source Daresbury...
Three-dimensional structures derived from X-ray diffraction of protein crystals provide a wealth information. Features and interactions important for the function macromolecules can be deduced catalytic mechanisms postulated. Still, many questions remain, example regarding metal oxidation states interpretation `mystery density', i.e. ambiguous or unknown features within electron density maps, especially at ∼2 Å resolutions typical most macromolecular structures. Beamline X26-C National...
A survey and analysis of Protein Data Bank (PDB) depositions from international synchrotron radiation facilities, based on the latest released PDB entries, are reported. The results (http://asdp.bnl.gov/asda/Libraries/) show that worldwide, every year since 1999, more than 50% deposited X-ray structures have used reaching 75% by 2003. In this web-based database, all entries among individual beamlines archived, synchronized with weekly release. Statistics regarding quality experimental data...
Native data, anomalous data at three wavelengths and an independent peak-wavelength set for SeMet-substituted protein have been collected from cryoprotected crystals of the TrpRS–adenylate product (TAM) complex to a resolution limit 1.7 Å. Independent phase sets were developed using SHARP improved by solvent flipping with SOLOMON molecular envelopes derived experimental densities for, respectively, SAD four different crystals, MAD their M(S)IRAS combinations native data....
This manuscript chronicles the evolution of software used originally to control a diffractometer at macromolecular crystallography beamline. The system has been augmented and rewritten. A modular carefully organized suite programs now handles whole experimental environment from single vantage point. It provides automatic logging experiment communication with user, all way an initial proposal perform work end data collection. included construction relational database organize details...
High throughput screening technologies such as acoustic droplet ejection (ADE) greatly increase the rate at which X-ray diffraction data can be acquired from crystals. One promising high application of ADE is to rapidly combine protein crystals with fragment libraries. In this approach, each soaks into a crystal either directly on collection media or moving conveyor belt then delivers beam. By simultaneously handling multiple combined specimens, these techniques relax automounter duty-cycle...