A5021392314- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Hepatitis Viruses Studies and Epidemiology
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- Liver Disease and Transplantation
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Medical Research and Treatments
- COVID-19 and healthcare impacts
- COVID-19 Impact on Reproduction
- RNA Interference and Gene Delivery
- Traditional Chinese Medicine Studies
- Drug-Induced Hepatotoxicity and Protection
- COVID-19 diagnosis using AI
Huashan Hospital
2020-2025
Fudan University
2020-2025
National Institute for Communicable Disease Control and Prevention
2022-2024
National Center for Infectious Diseases
2022
Background & Aims: A notable proportion of CHB patients undergoing PEG-IFNα based therapy experience lagged serum HBeAg and/or HBV DNA disappearance in achieving HBsAg loss. In this study, we explored the molecular mechanisms behind clinical phenomenon, offering novel insights into sustainability chronic infection.
Chronic infection with hepatitis B virus (HBV) is associated liver cirrhosis and hepatocellular carcinoma. Upon of hepatocytes, HBV covalently closed circular DNA (cccDNA) exists as histone-bound mini-chromosome, subjected to transcriptional regulation similar chromosomal DNA. Here we identify high mobility group AT-hook 1 (HMGA1) protein a positive regulator transcription that binds conserved ATTGG site within enhancer II/core promoter (EII/Cp) recruits factors FOXO3α PGC1α. HMGA1-mediated...
Abstract Prolonged severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection has received much attention since it is associated with mortality and hypothesized as the cause of long COVID‐19 emergence a new variant concerns. However, prediction model for accurate prolonged still lacking. A total 2938 confirmed patients diagnosed by positive reverse transcriptase‐polymerase chain reaction tests were recruited retrospectively. This study cohort was divided into training set (70%...
Abstract Introduction The SARS-CoV-2 Omicron variant has decreased virulence and pathogenicity, yet the number of infections worldwide is unprecedentedly high, with rather high mortality severe disease rate. Chronic kidney (CKD) patients are particularly vulnerable to have unique clinical outcomes. Methods We retrospectively collected data from 2140 hospitalized infection March 29, 2022, May 17, 2022. Demographic characteristics, ancillary examination results, treatments were described....
Hepatitis B surface antigen (HBsAg) loss, namely, the functional cure, can be achieved through pegylated interferon (PEG-IFN)-based therapy. However, it is an unignorable fact that a small proportion of patients who cure develop HBsAg reversion (HRV) and related factors are not well described. A total 112 PEG-IFN-induced loss were recruited. HBV biomarkers biochemical parameters examined dynamically. RNA levels assessed in cross-sectional analysis. The primary endpoint was HRV, defined as...
The long-term impact, incidence and risk factors of thyroid dysfunction in chronic hepatitis B (CHB) patients receiving pegylated interferon (IFN) alpha (PegIFN-alpha) therapy remain unclear. We aim to investigate the safety CHB PegIFN-alpha. A retrospective observational study 425 with normal baseline function was carried out. Patients were followed up over 10 years assess after IFN. At end IFN therapy, 67 (15.8%) had developed dysfunction, 31 (46.3%) hyperthyroidism 64.4% presented...
HBx plays a significant role in the cccDNA epigenetic modification regulating hepatitis B virus (HBV) life cycle and hepatocyte proliferation carcinogenesis. By using sleeping-beauty transposon system, we constructed tetracycline-induced HBx-expressing stable cell line, SBHX21. with HiBiT tag can be quickly detected utilizing NanoLuc-based detection system. screening drug library SBHX21 cells, identified estradiol benzoate as novel anti-HBx agent. Estradiol also markedly reduced production...
Myeloid-derived suppressor cells (MDSCs) and CD4+ regulatory T (Tregs) expand during chronic hepatitis B virus (HBV) infection inhibit antiviral immunity. However, the relationship between effect frequencies of those immune suppressive after pegylated interferon α-2a (PegIFNα-2a) therapy is not clearly understood. This study aimed to investigate contribution monocytic MDSCs (mMDSCs) Tregs functional cure (HBsAg seroclearance) PegIFNα-2a evaluate on these cells.Flow cytometry analysis was...
The variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged repeatedly, especially the Omicron strain which is extremely infectious, so early identification patients who may develop critical illness will aid in delivering proper treatment and optimizing use resources. We aimed to validate a practical scoring model at hospital admission for predicting with infection illness.A total 2,459 were enrolled this retrospective study. Univariate multivariate logistic...
Low-level viremia is usually defined as a detectable but lower than 2000 IU/mL hepatitis B virus DNA level after 12 months or longer duration of antiviral therapy in chronic patients. In this study, we aimed to clarify the factors associated with lowlevel patients during long-term monotherapy tenofovir disoproxil fumarate entecavir.Chronic having received entecavir treatment for more were enrolled from October 2019 2021 at tertiary hospital Shanghai, China. accordance their levels, grouped...
The study aims to evaluate the effectiveness of a tenofovir alafenamide fumarate (TAF) and pegylated interferon alfa (PegIFN-α) regimen compared disoproxil (TDF) PegIFN-α therapy in patients with chronic hepatitis B (CHB).Patients who were treated combination TAF or TDF retrospectively enrolled. primary outcome measured was HBsAg loss rate. rates virological response, serological response for HBeAg, normalization alanine aminotransferase (ALT) also calculated. cumulative incidences between...
Background. Nucleotide analogues (NTs) monotherapy may have a more significant effect on reducing hepatitis B surface antigen (HBsAg) than nucleoside (NSs) due to their immunomodulatory function. However, this superiority remains unknown when combined with PEGylated interferon α (PegIFNα). Therefore, study aimed explore whether NTs antiviral effects NSs in combination therapy PegIFNα. Methods. Chronic (CHB) patients treated PegIFNα plus nucleos(t)ide (NAs) were retrospectively recruited....
Abstract Background: Nucleotide analogues (NTs) monotherapy may have a greater effect on reducing hepatitis B surface antigen (HBsAg) than nucleoside (NSs) due to their immunomodulatory function. However, this superiority remains unknown when combined with pegylated interferon α (PegIFNα). The study aimed explore whether NTs antiviral effects NSs in combination therapy PegIFNα. Methods: Chronic (CHB) patients treated PegIFNα plus nucleos(t)ide (NAs) were retrospectively recruited. Efficacy...
Objectives. The rapidly evolving organ failure and high short-run mortality of acute-on-chronic liver (ACLF) are inseparable from the role systemic inflammatory response. S100A8 S100A9 associated with excessive cytokine storm play a decisive part within process inflammation. We aimed to clarify them in predicting prognosis hepatitis B virus-related ACLF (HBV-ACLF). Methods. levels were analyzed plasma 187 transplant-free HBV-ACLF patients, 28 healthy controls 40 chronic (CHB) patients. mRNAs...
Abstract Background Since March 2022, the SARS-CoV-2 Omicron variant, mainly BA.2 sub-lineage, has swept throughout Shanghai, China and caused a wide range of infections. Patients with chronic kidney disease (CKD) are particularly vulnerable to subvariant have unique clinical manifestations outcomes, which not been studied yet. Methods We retrospective collected data from 2140 hospitalized patients infection 29, May 17, 2022. Demographic characteristics, symptoms, ancillary examination...