A5085200746- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Listeria monocytogenes in Food Safety
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Water Treatment and Disinfection
- Fecal contamination and water quality
- Bacterial biofilms and quorum sensing
- Environmental DNA in Biodiversity Studies
- Legionella and Acanthamoeba research
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Microbial infections and disease research
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- Animal Virus Infections Studies
- Parasitic Infections and Diagnostics
- Probiotics and Fermented Foods
- Aquaculture disease management and microbiota
- Microbial Community Ecology and Physiology
- Bacillus and Francisella bacterial research
- Identification and Quantification in Food
Institute of Environmental Science and Research
2016-2025
University of Canterbury
2006-2018
Bacteriophages infecting Salmonella spp. were isolated from sewage using soft agar overlays containing three serovars and assessed with regard to their potential control food-borne salmonellae. Two distinct phages, as defined by plaque morphology, structure host range, obtained a single sample of screened sewage. Phage FGCSSa1 had the broadest range six eight isolates neither two Escherichia coli isolates. Under optimal growth conditions for S. Enteritidis PT160, phage infection resulted in...
Resource-limited settings and supply chain difficulties faced throughout the COVID-19 pandemic prompted development of rapid alternative methods detecting SARS-CoV-2. These include reverse-transcription loop-mediated isothermal amplification (RT-LAMP), recombinase polymerase (RT-RPA), CRISPR-Cas12a fluorescence detection. We describe RT-LAMP, RT-RPA, assays for detection N E-gene amplicons SARS-CoV-2 optimization various assay components, including incubation temperatures, Cas12a enzymes,...
Wastewater treatment plants (WWTPs) receive and treat large volumes of domestic, industrial, urban wastewater containing pathogenic nonpathogenic microorganisms, chemical compounds, heavy metals, other potentially hazardous substances. WWTPs play an essential role in preserving human, animal, environmental health by removing many these toxic infectious agents, particularly biological hazards. contains complex consortiums bacterial, viral, archaeal, eukaryotic species, while bacteria WWTP...
Livestock-associated Staphylococcus aureus ST398 is a highly pathogenic species that causes infections in wide variety of animals, including humans. The bacteriophage (phage) vB_SauS_S1 was isolated originally using strain as its "isolating host", then the spot tests showed it able to infect 73.33% (22/30) isolates. Phage S1 assigned temperate phage based on genome analysis and phenotypic validation. Phylogenetic closely related phages tp310-2 SA137ruMSSAST121PVL. Following infection by S1,...
To use bacteriophages (phages) to control food-borne pathogenic bacteria, it will be necessary determine the conditions allowing optimal activity. start exploring these conditions, a Salmonella phage (P7) and Campylobacter (Cj6) were incubated with their respective hosts at 24 degrees C for up 2 h varying host cell concentrations, surviving cells enumerated. A quadratic polynomial equation was fitted inactivation data contour maps of against log(10) concentrations plotted. Inactivation by P7...
A previously described phage infecting Escherichia coli O157:H7 was added to raw and cooked beef pieces at concentrations ranging from 10 1 –10 8 plaque forming units/cm 2 either low (<100 CFU/cm ) or high (10 4 of host bacterial cells. Incubation for up 24 h performed 5℃ 24℃ simulate refrigerated room temperature storage/temperature abuse. Surviving bacteria were enumerated during the incubation period, with phages being counted first last sampling times. Significant reductions E. order...
To isolate and characterize bacteriophages (phages) that infect the foodborne pathogen Bacillus cereus.Two phages were isolated from soil based on their ability to form plaques four indicator hosts including thuringiensis subsp. israelensis, three isolates of B. cereus. The purified characterized by morphology, host range, single-step growth curves restriction enzyme digestion profiles. appeared be Myoviridae family structure in electron micrographs. lysed bacteria several species, produced...
Aim To isolate and characterize listeriaphages from seafood environments. Methods Results Listeriaphages (phages) isolated environments were distinguished by physical biological techniques including restriction digestion of phage DNA. Three phages belonged to order Caudovirales showed psychrotrophic characteristics. The had broad host ranges against 23 Listeria strains productive infection or at least adsorption. At 15 ± 1°C, adsorption rate constants the three ranged 8·93 × 10−9 3·24 10−11...
Bacteriophage-encoded endolysins have been identified as antibacterial candidates. However, the development of mainstream agents first requires a comprehensive biochemical understanding. This study defines atomic structure and enzymatic function Escherichia coli O157:H7 phage FAHEc1 endolysin, LysF1. Bioinformatic analysis suggests this endolysin belongs to T4 Lysozyme (T4L)-like family proteins contains highly conserved catalytic triad. We then solved LysF1 with x-ray crystallography 1.71...