A5061187992- Thyroid Disorders and Treatments
- S100 Proteins and Annexins
- Cardiac electrophysiology and arrhythmias
- Nutrition and Health in Aging
- Cardiac Arrhythmias and Treatments
- Musculoskeletal pain and rehabilitation
- Cardiovascular Syncope and Autonomic Disorders
- Neuropeptides and Animal Physiology
- Thyroid Cancer Diagnosis and Treatment
- Scoliosis diagnosis and treatment
- Protease and Inhibitor Mechanisms
- Computational Drug Discovery Methods
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Neuroscience and Neuropharmacology Research
- Hypothalamic control of reproductive hormones
- Atrial Fibrillation Management and Outcomes
- Heat shock proteins research
- Metabolism, Diabetes, and Cancer
- Melanoma and MAPK Pathways
- Neurogenesis and neuroplasticity mechanisms
- RNA Interference and Gene Delivery
- Enzyme function and inhibition
- Heparin-Induced Thrombocytopenia and Thrombosis
- Fungal Plant Pathogen Control
Hokkaido University
2024
Shimane University
2021-2022
University of Shimane
2021
City Hospital
2020
Nagoya City University
2009
Tottori University
1999
University of Yamanashi
1986-1996
Mie University
1981-1983
Shinshu University
1981
N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) and its derivatives are putative calmodulin antagonists that bind to inhibit Ca2+/calmodulin-regulated enzyme activities. Autoradiographic studies using tritiated W-7 showed this compound penetrates the cell membrane, is distributed mainly in cytoplasm, inhibits proliferation of Chinese hamster ovary K1 (CHO-K1) cells. Cytoplasmic [3H]W-7 was excluded completely within 6 hr after removal from culture medium....
Iodide is concentrated to a much lesser extent by papillary thyroid carcinoma as compared with the normal gland. The Na+/I- symporter (NIS) primarily responsible for uptake of iodide into cells. Our objective was compare NIS mRNA and protein expression in carcinomas those specimens thyroid. Northern blot analysis revealed 2.8-fold increase level versus Immunoblot using anti-human antibody that produced glutathione S-transferase fusion containing (amino acids 466-522) showed at 77 kD....
Two different calcium-binding proteins, S-100 and calmodulin, have been isolated from bovine brain by calcium-dependent affinity chromatography on calmodulin antagonist coupled to Sepharose. Calmodulin N-(6-aminohexyl)-5-chloro-l-napthdenesulfonamide (W-7) has epoxy-activated Sepharose 6B or cyanogen bromide-acitivated 4B. S-100-like protein bound W-7 in the presence of Ca2+ was eluted with buffer containing 4 m~ ethylene glycol bis(j3-aminoethyl ether)-N,NJ?’,iV-tetraacetic acid (EGTA)...
We have purified S-100 protein from bovine brain using Ca2+-dependent affinity chromatography on
We investigated the glucose transporter mRNAs expressed in FRTL5, a rat thyroid cell line, and their regulation by TSH means of polymerase chain reaction. FRTL5 cells as well tissue three types mRNAs: GLUT1 or erythrocyte/HepG2/brain isoform, GLUT2 pancreatic beta-cell/liver GLUT4 muscle/fat isoform. mRNA predominated, was minor, expression faint. Incubation with induced time- concentration-dependent increase levels, while levels were decreased. The response to evident at 3 h, maximal...
Postoperative adhesions are the major cause of postoperative complications including intestinal obstruction, infertility, and chronic pelvic pain. In order to reduce adhesions, Terumo Corporation (Tokyo, Japan) has developed an adhesion barrier system (TCD-11091) which is easy use at treatment site in various surgical procedures laparoscopic surgeries. We conducted a prospective randomized single-blind study patients who underwent laparotomy with ileostomy. One hundred twenty-six were...
To investigate the regulation of expression TSH receptor (TSHR) in extrathyroidal tissues, level TSHR messenger RNA (mRNA) and TSH-dependent signal transduction were determined isolated rat adipocytes cultured preadipocytes. The epididymal, sc, perirenal, but not interscapular brown adipose possessed mRNA increased cAMP responses to thus used as source Morphological analysis revealed that combination insulin T3 most effectively caused differentiation These differentiated preadipocytes...
The effects of a novel compound, 1-(3-chloroanilino)-4-phenylphthalazine (MY-5445), on cyclic nucleotide metabolism and in vitro aggregation human platelets were investigated. concentrations MY-5445 producing 50% inhibition platelet induced by 3 microM ADP, micrograms/ml collagen 100 arachidonic acid 0.07, 0.02 0.17 microM, respectively. Addition significantly elevated GMP content but had no effect AMP content, suggesting that the drug affects principally platelet. Although either adenylate...
Low back pain (LBP) is a common complaint in the elderly Japanese population. Although previous studies showed that height loss was associated with LBP, it remains unclear whether LBP body composition. The objective of present study to investigate composition and physical characteristics, including loss, were LBP. retrospectively registered, participants 2212 community-dwelling people aged over 60 years who participated Shimane CoHRE 2016. We investigated presence parameters (muscle, fat,...
Sarcopenia is intricately related to aging associated diseases, such as neuropsychiatric disorders, oral status, and chronic diseases. Dementia depression are interconnected also sarcopenia. The preliminary shift from robust sarcopenia (i.e., pre-sarcopenia) an important albeit underdiscussed stage the focus of this study. Identifying factors with pre-sarcopenia may lead prevention. To separately examine effects dementia on pre-sarcopenia/sarcopenia, we conducted multiple analyses. This...
Free radicals play an important role in the inflammatory process of sepsis. We hypothesized that edaravone, a novel free radical scavenger, can suppress pathophysiological events and prolong survival neonatal sepsis cecal ligation perforation (CLP) model. Of 32 3-day-old anesthetized mechanically ventilated piglets, 11 received CLP only, 10 edaravone treatment starting 30 min after CLP, constituted sham (control) group. Mean arterial pressure (MAP), heart rate, cardiac output, blood gas,...
We report here the ultrastructural localization of S-100b protein-like immunoreactivity in centriole, cilia, and basal body. Duodenum trachea guinea pigs rats were fixed immunostained by protein A-gold method. All centrioles, bodies observed showed clear immunoreactivity. Specific colloidal gold particles located over microtubules these cell organelles. However, other scattered throughout cytoplasm devoid Although functional significance remains to be elucidated, present results introduce...
Narrow pelvic inlet plane area and obesity as risk factors for anastomotic leakage after intersphincteric resection
Studies on the association between sodium-to-potassium (Na/K) ratio changes and blood pressure (BP) among older adults are limited. This 7-year longitudinal study examined Na/K (evaluated using spot urine tests) BP Japanese adults. Data were collected from 432 participants (mean age: 70.3±4.4; range: 65−84 years) in 2012 2019. Changes over 7 years calculated by subtracting baseline values noted during a follow-up survey. The median systolic diastolic (SBP) (DBP) after 4 (IQR, −7, 14) −1 −9,...
The expression of beta 2-adrenergic receptor (AR) mRNA was investigated in normal and neoplastic human thyroid tissues. A combination techniques for reverse transcribing into cDNA the incorporation 32P-gamma ATP polymerase chain reaction (PCR)-generated fragments allowed us to detect 2-AR surgically excised specimens. levels generated by PCR adenomas cancers were 3.3 6.9 times, respectively, as high that These findings suggest level is correlated with extent differentiation present study...
Depressive symptoms pose the risk of transitioning to depression, which increases mortality rates among older adults. In rural Japan, where population is aging, community bonds are dwindling, raising experiencing depressive symptoms. Building social support (SS) gaining attention as a means prevent depression; however, there lack research on suitable types SS resources. The present study hypothesized that from or family neighbors would reduce symptom community‐dwelling women and men in...
Parvalbumin (PA), one of the Ca2+-binding neuronal marker proteins, has been revealed to exist in myelinated axons posterior root spinal cord and peripheral nerve rats. To investigate role PA for genesis diabetic neuropathy, levels sciatic normal streptozotocin-induced rats were measured by radioimmunoassay (RIA) PA. The immunohistochemical distribution from both groups was also studied. RIA that significantly decreased when compared with those However, contents S-100 protein, another type...