A5082084368- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Fermentation and Sensory Analysis
- Ubiquitin and proteasome pathways
- Cell death mechanisms and regulation
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- Studies on Chitinases and Chitosanases
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- DNA Repair Mechanisms
- Nuclear Receptors and Signaling
- Toxin Mechanisms and Immunotoxins
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Calpain Protease Function and Regulation
- Cancer-related molecular mechanisms research
- Transgenic Plants and Applications
Shanghai Micro Satellite Engineering Center
2014-2025
Fudan University
2013-2025
State Key Laboratory of Genetic Engineering
2008-2024
Shanghai Fudan Microelectronics (China)
2021-2022
East China University of Science and Technology
2020-2022
Ministry of Agriculture and Rural Affairs
2022
Agricultural Genomics Institute at Shenzhen
2022
Chinese Academy of Agricultural Sciences
2022
Kunming Medical University
2021
Huashan Hospital
2017
A genetic interaction network containing approximately 1000 genes and 4000 interactions was mapped by crossing mutations in 132 different query into a set of 4700 viable gene yeast deletion mutants scoring the double mutant progeny for fitness defects. Network connectivity predictive function because often occurred among functionally related genes, similar patterns tended to identify components same pathway. The exhibited dense local neighborhoods; therefore, position on partially is other...
Kluyveromyces marxianus, the known fastest-growing eukaryote on earth, has remarkable thermotolerance and capacity to utilize various agricultural residues produce low-cost bioethanol, hence is industrially important resolve imminent energy shortage crisis. Currently, poor ethanol tolerance hinders its operable application in industry, it necessary improve K. marxianus' resistance unravel underlying systematical mechanisms. However, this been seldom reported date. We carried out a wild-type...
Soy leghemoglobin, when bound to heme, imparts a meat-like color and flavor can serve as substitute for animal-derived proteins. Enhancing cellular heme synthesis improves the recombinant expression of leghemoglobin in yeast. To achieve high-level A (LBA) Kluyveromyces marxianus , food-safe yeast, large-scale modules were transferred into K. using yeast artificial chromosomes (KmYACs). These contained up 8 native heterologous genes promote supply precursors downstream synthesis. Next, eight...
Abstract Kluyveromyces marxianus is a food‐safe yeast with great potential for producing heterologous proteins. Improving the yield in K. remains challenge and incorporating large‐scale functional modules poses technical obstacle engineering. To address these issues, linear circular artificial chromosomes of (KmYACs) were constructed loaded disulfide bond formation from Pichia pastoris or . These contained up to seven genes maximum size 15 kb. KmYACs carried telomeres either Tetrahymena...
Taking into account its thermotolerance, high growth rate, and broad substrate spectrum, Kluyveromyces marxianus can be considered an ideal consolidated bioprocessing (CBP). A major obstacle to ethanol production using K. is the low of lignocellulolytic enzymes, which reduces cellulose hydrolysis production. Thus, further improvement enzyme expression secretion essential. To improve inulinase promoter signal sequence from was optimized through mutagenesis. T(-361)A mutation inside promoter,...
Kluyveromyces marxianus is an emerging yeast cell host for diverse products, but multiple-gene expression in K. faces challenges due to limited current knowledge of cis-regulatory elements and insertion loci. Our previous study transferred alien Saccharomyces cerevisiae chromosome I (R1) into marxianus, resulting the creation monochromosomal hybrid KS-R1. All R1 genes were actively transcribed, providing a series loci with varying transcriptional activities. Here, we explore use as novel...
N-glycosylation is a prevalent post-translational modification in eukaryotes, essential for regulating protein secretion. In Saccharomyces cerevisiae, glycosylation mutants have been shown to enhance the secretion of heterologous glycosylated proteins. However, whether these can also increase non-glycosylated proteins and growth defects associated with mutations be mitigated remains unclear. This study aimed characterize optimize enhanced secretory expression promising yeast host...
In this study, we employed directed evolution and site-directed mutagenesis to screen thermostable mutants of a family 11 xylanase from Neocallimastix patriciarum, found that the thermostability specific activity are both enhanced when mutations (G201C C60A) take place in interior hydrophobic region enzyme. Far-ultraviolet circular dichroism analysis showed melting temperatures (T(m)) G201C C60A-G201C higher than wild type by about 10 12 degrees C, respectively. At 72 their activities 4 6...
Posttranslational modification of p53 is a critical event in regulating the expression its target genes. acetylated at lysine 120 (K120) by acetyltranferases Tip60 (KAT5) and hMOF (KAT8) response to DNA damage. Identification cofactors for these two enzymes will shed light on mechanism which cells make choice between cell-cycle arrest apoptosis. It has been reported that ING5, member inhibitor growth (ING) family, involved p53-dependent pathways, but exact role unknown. In this study, we...
MicroRNAs (miRNAs) play important roles in various biological processes and are closely associated with the development of cancer. In fact, aberrant expression miRNAs has been implicated numerous cancers. cervical cancer, miR-203 levels decreased, although cause this remains unclear. study, we investigate molecular mechanisms regulating gene transcription. We identify transcription start site by 5' rapid amplification cDNA ends subsequently promoter region. Promoter analysis revealed that...
Abstract Background Porcine Parvovirus (PPV) is a Parvovirinae virus that can cause embryonic and fetal loss death mummification in affected pigs. Unlike conventional vaccines, virus-like particles (VLPs) inherit the natural structure of their authentic virions highly immunostimulatory induce strong humoral immune T cell responses with no risk pathogenicity. The production PPV VLPs still challenge based on traditional expression platforms due to low yields high culture costs. Kluyveromyces...
This study explored the application of
Increasing evidence suggests that lysosomal cysteine proteases cathepsins contribute to the progression of cell apoptosis. Here we found apoptosis ovarian cancer cells OV‐90 triggered by TNF was cathepsin B‐depended. Two B binding proteins, bikunin and TSRC1, were identified yeast two‐hybrid method interactions confirmed in vitro vivo. Overexpression could suppress TNF‐induced cells, TSRC1 overexpression had an opposite effect on The presented results suggest its interacting are involved...
Abstract Background DNA damage response (DDR) plays pivotal roles in maintaining genome integrity and stability. An effective DDR requires the involvement of hundreds genes that compose a complicated network. Because is highly conserved evolution, studies lower eukaryotes can provide valuable information to elucidate mechanism higher organisms. Fission yeast ( Schizosaccharomyces pombe ) has emerged as an excellent model for research recent years. To identify novel involved DDR, we screened...