A5101721408- Lepidoptera: Biology and Taxonomy
- Plant and animal studies
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Environmental DNA in Biodiversity Studies
- Species Distribution and Climate Change
- Forest Insect Ecology and Management
- Insect and Arachnid Ecology and Behavior
- Insect-Plant Interactions and Control
- Ecology and Vegetation Dynamics Studies
- Insect Resistance and Genetics
- Rabies epidemiology and control
- Human-Animal Interaction Studies
- Wildlife Ecology and Conservation
- Coleoptera Taxonomy and Distribution
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Hymenoptera taxonomy and phylogeny
- Crustacean biology and ecology
- Fossil Insects in Amber
- Marine Biology and Ecology Research
- Insect Utilization and Effects
- Isotope Analysis in Ecology
- Plant Virus Research Studies
Capital Normal University
2016-2025
Langfang Normal University
2020
Science for Life Laboratory
2011-2013
KTH Royal Institute of Technology
2009-2013
Institute of Zoology
2008
Chinese Academy of Sciences
2008
Kyoto University
2006-2007
There is no generally accepted picture of where, when, and how the domestic dog originated. Previous studies mitochondrial DNA (mtDNA) have failed to establish time precise place origin because lack phylogenetic resolution in so far studied control region (CR), inadequate sampling. We therefore analyzed entire genomes for 169 dogs obtain maximal CR 1,543 across Old World a comprehensive geographical diversity. Hereby, detailed origins can first be suggested. obtained evidence that has single...
The advent in high-throughput-sequencing (HTS) technologies has revolutionized conventional biodiversity research by enabling parallel capture of DNA sequences possessing species-level diagnosis.However, polymerase chain reaction (PCR)-based implementation is biased the efficiency primer binding across lineages organisms.A PCR-free HTS approach will alleviate this artefact and significantly improve upon multi-locus method utilizing full mitogenomes.Here we developed a novel multiplex...
Abstract Background Explicit evolutionary models are required in maximum-likelihood and Bayesian inference, the two methods that overwhelmingly used phylogenetic studies of DNA sequence data. Appropriate selection nucleotide substitution is important because use incorrect can mislead inference. To better understand performance different model-selection criteria, we 33,600 simulated data sets to analyse accuracy, precision, dissimilarity, biases hierarchical likelihood-ratio test, Akaike...
Abstract Background A well-informed choice of genetic locus is central to the efficacy DNA barcoding. Current barcoding in animals involves use 5' half mitochondrial cytochrome oxidase 1 gene ( CO1 ) diagnose and delimit species. However, there no compelling a priori reason for exclusive focus on this region, it has been shown that performs poorly certain animal groups. To explore alternative regions, we compared universal region with other protein-coding genes eutherian mammals. Four...
Abstract Rapid and accurate identification of species is required for the biological control pest N octuoidea moths. DNA barcodes thin‐film biosensor chips are two molecular approaches that have gained wide attention. Here, we compare these methods a limited number moth species. Based on commonly used mitochondrial gene cytochrome c oxidase I (the standard barcode animal species), 14 probes were designed synthesized shared by national nature reserves in B eijing H ebei, C hina. Probes ranged...
Abstract Reliable assignment of an unknown query sequence to its correct species remains a methodological problem for the growing field DNA barcoding. While great advances have been achieved recently, identification from barcodes can still be unreliable if relevant biodiversity has insufficiently sampled. We here propose new notion membership barcoding—fuzzy membership, based on fuzzy set theory—and illustrate successful application four real data sets (bats, fishes, butterflies and flies)...
Integrative taxonomy is central to modern and systematic biology, including behavior, niche preference, distribution, morphological analysis, DNA barcoding. However, decades of use demonstrate that these methods can face challenges when used in isolation, for instance, potential misidentifications due phenotypic plasticity methods, incorrect identifications because introgression, incomplete lineage sorting, horizontal gene transfer Although researchers have advocated the integrative...
DNA barcoding as a method for species identification is rapidly increasing in popularity. However, there are still relatively few rigorous methodological tests of barcoding. Current distance-based methods frequently criticized treating the nearest neighbor closest relative via raw similarity score, lacking an objective set criteria to delineate taxa, or being incongruent with classical character-based taxonomy. Here, we propose artificial intelligence–based approach—inferring membership...
Studies of mitochondrial DNA (mtDNA) diversity indicate explicitly that dogs were domesticated, probably exclusively, in southern East Asia. However, Southwest Asia (SwAsia) has had poor representation and geographical coverage these studies. Other studies based on archaeological genome-wide SNP data have suggested an origin SwAsia. Hence, it been suspected mtDNA evidence for this scenario may remained undetected. In the first comprehensive investigation genetic among SwAsian dogs, we...
Dogs were present in pre-Columbian America, presumably brought by early human migrants from Asia. Studies of free-ranging village/street dogs have indicated almost total replacement these original European dogs, but the extent to which Arctic, North and South American breeds are descendants population remains be assessed. Using a comprehensive phylogeographic analysis, we traced origin mitochondrial DNA lineages for Inuit, Eskimo Greenland Alaskan Malamute, Chihuahua, xoloitzcuintli perro...
Summary Species identification via DNA barcodes has recently become an important and routine task in many biodiversity projects using sequence data. Here, we present BarcodingR, integrated software package that provides a comprehensive implementation of species methods, including artificial intelligence, fuzzy‐set, Bayesian kmer‐based are not readily available other packages. BarcodingR additionally new functions for barcode evaluation, barcoding gap analysis, delimitation comparison...
Gelechioidea represents the most diverse superfamily of tiny boring pests in Lepidoptera that pose a serious threat to agricultural and forestry economic crops. However, lack high-quality genome highly specialized species makes it difficult draw general conclusions about mechanism close binding relationship between In this study, based on second- third-generation sequencing reads, we constructed chromosome-level for Atrijuglans aristata, pest, specifically harms green husk cultivated...
Sorghum is a key global crop with substantial economic importance. Implementing green pest management for sorghum crucial promoting ecological balance and reducing reliance on chemical pesticides. This study assesses the impact of arthropod biodiversity yield quality. Over two years, using Malaise trapping DNA metabarcoding, we found that significantly enhanced diversity, increasing species richness by 5.63% shifting composition, notably abundance Hymenoptera. Although metrics were higher in...
Unlike distinct species, closely related species offer a great challenge for phylogeny reconstruction and identification with DNA barcoding due to their often overlapping genetic variation. We tested sibling group of pine moth pests in China standard cytochrome c oxidase subunit I (COI) gene two alternative internal transcribed spacer (ITS) genes (ITS1 ITS2). Five different phylogenetic/DNA analysis methods (Maximum likelihood (ML)/Neighbor-joining (NJ), "best close match" (BCM), Minimum...
Fruit flies in the family Tephritidae are economically important pests that have many species complexes. DNA barcoding has gradually been verified as an effective tool for identifying a wide range of taxonomic groups, and there several publications on rapid accurate identification fruit based this technique; however, comprehensive analyses large new taxa effectiveness rare. In study, we evaluated COI barcode sequences diagnosis using 1426 73 Bactrocera distributed worldwide. Tree-based...
We tested the performance of DNA barcoding in Acridoidea and attempted to solve species boundary delimitation problems selected groups using COI barcodes. Three analysis methods were applied reconstruct phylogeny. K2P distances used assess overlap range between intraspecific variation interspecific divergence. "Best match (BM)", "best close (BCM)", "all barcodes (ASB)" "back-propagation neural networks (BP-based method)" utilized test success rate identification. Phylogenetic concept network...
Pine moths (Lepidoptera; Bombycoidea; Lasiocampidae: Dendrolimus spp.) are among the most serious insect pests of forests, especially in southern China. Although COI barcodes (a standardized portion mitochondrial cytochrome c oxidase subunit I gene) can distinguish some members this genus, evolutionary relationships three morphospecies punctatus, D. tabulaeformis and spectabilis have remained largely unresolved. We sequenced whole genomes eight specimens, including punctatus wenshanensis....
Abstract For some groups of organisms, DNA barcoding can provide a useful tool in taxonomy, evolutionary biology, and biodiversity assessment. However, the efficacy depends on degree sampling per species, because large enough sample size is needed to reliable estimate genetic polymorphism for delimiting species. We used simulation approach examine effects four estimators related barcoding: mismatch distribution, nucleotide diversity, number haplotypes, maximum pairwise distance. Our results...
Abstract Dendrolimus spp. are important destructive pests of conifer forests, and punctatus Walker (Lepidoptera; Lasiocampidae) is the most widely distributed species. During periodic outbreaks, this species said to make “fire without smoke” because large areas pine forest can be quickly heavily damaged. Yet, little known about molecular mechanisms that underlie unique ecological characteristics insect. Here, we combined Pacific Biosciences (PacBio) RSII single‐molecule long reads...
Abstract Background Over the last decade, rapid development of high-throughput sequencing platforms has accelerated species description and assisted morphological classification through DNA barcoding. However, current barcoding methods cannot obtain full-length barcode sequences due to read length limitations (e.g. a maximum 300 bp for Illumina’s MiSeq system), or are hindered by relatively high cost low output number eight million reads per cell PacBio’s SEQUEL II system). Results Pooled...