A5083948455- SARS-CoV-2 detection and testing
- Cancer Cells and Metastasis
- Biosensors and Analytical Detection
- Pancreatic and Hepatic Oncology Research
- Extracellular vesicles in disease
- Multiple Myeloma Research and Treatments
- Advanced biosensing and bioanalysis techniques
- Phagocytosis and Immune Regulation
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 and COVID-19 Research
- Membrane Separation Technologies
- Mycobacterium research and diagnosis
- Ferroptosis and cancer prognosis
- T-cell and B-cell Immunology
- Gut microbiota and health
- Cancer Treatment and Pharmacology
- Immune Cell Function and Interaction
- Caveolin-1 and cellular processes
- Viral gastroenteritis research and epidemiology
- Immune cells in cancer
- Cancer Genomics and Diagnostics
- Nanopore and Nanochannel Transport Studies
- Protein Degradation and Inhibitors
- COVID-19 Clinical Research Studies
University of Miami
2021-2024
Sylvester Comprehensive Cancer Center
2021-2024
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in wastewater has been used to track community infections of coronavirus disease-2019 (COVID-19), providing critical information for public health interventions. Since levels are dependent upon human inputs, we hypothesize that tracking can be improved by normalizing concentrations against indicators waste [Pepper Mild Mottle Virus (PMMoV), β-2 Microglobulin (B2M), and fecal coliform]. In this study, analyzed SARS-CoV-2 from two...
Natural killer (NK) cells are cytotoxic lymphocytes that accumulate within the tumor microenvironment and generally considered to be antitumorigenic. Using single-cell RNA sequencing functional analysis of multiple triple-negative breast cancer (TNBC) basal samples, we observed a unique subcluster Socs3 high CD11b − CD27 immature NK were present only in TNBC samples. These tumor-infiltrating expressed reduced granzyme signature and, mice, responsible for activating stem through Wnt...
Methods of wastewater concentration (electronegative filtration (ENF) versus magnetic bead-based (MBC)) were compared for the analysis severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), beta-2 microglobulin, and human coronavirus OC43. Using ENF as method, two quantitative polymerase chain reaction (qPCR) analytical methods also compared: volcano second generation (V2G)-qPCR reverse transcriptase (RT)-qPCR measuring three different targets virus responsible COVID-19 illness (N1,...
TPS713 Background: PDAC is characterized by its innate and acquired resistance to both MAPK pathway inhibition immune checkpoint (e.g. PD-1/PD-L1) (ICI) via multiple mechanisms. In preclinical models of PDAC, combined MEK STAT3 (MEKi+STAT3i) uncovers stromal plasticity attenuating cancer-associated fibroblasts (CAF) with IL-6/CXCL1-secretory phenotypes while enriching for Ly6a/CD34-expressing CAF mesenchymal stem cell-like features. This remodeling heterogeneity associated a striking...
Wastewater-based epidemiology (WBE) has been utilized to track community infections of Coronavirus Disease 2019 (COVID-19) by detecting RNA the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), within samples collected from wastewater. The correlations between and wastewater measurements can potentially change as SARS-CoV-2 evolves into new variations mutating. This study analyzed indicators human waste in two sewersheds different scales (University Miami (UM) campus Miami-Dade...
Abstract Background Pancreatic cancer is one of the most difficult cancers to detect early and patients die from complications arising due distant organ metastases. The lack bona fide biomarkers primary reasons for late diagnosis pancreatic cancer. It a multifactorial disease warrants novel approach identify biomarkers. Methods In order characterize proteome, Extracellular vesicles (EVs) isolated different in vitro conditions mimicking tumor-microenvironment interactions between epithelial...
Tumor cells dissociate from the primary site and enter into systemic circulation (circulating tumor cells, CTCs) either alone or as microemboli (clusters); latter having an increased predisposition towards forming distal metastases than single CTCs. The formation of clusters is, in part, created by contacts between cell–cell junction proteins and/or cytokine receptor pairs with other such neutrophils, platelets, fibroblasts, etc. In present study, we provide evidence for extravesicular (EV)...
Wastewater-based surveillance (WBS) is a noninvasive, epidemiological strategy for assessing the spread of COVID-19 in communities. This was based upon wastewater RNA measurements viral target, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The utility WBS has motivated research to measure targets beyond SARS-CoV-2, including pathogens containing DNA. objective this study establish necessary steps isolating DNA from by modifying long-standing RNA-specific extraction workflow...
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Background: Current strategies in circulating tumor cell (CTC) isolation pancreatic cancer heavily rely on the EpCAM and cytokeratin status. is generally not considered a good marker given its transitory change during Epithelial to Mesenchymal Transition (EMT) or reverse EMT. There need identify other surface markers capture complete repertoire of PDAC CTCs. The primary objective study characterize alternate biomarkers CTCs that express low negligible levels patients. Methods: Flow cytometry...
Abstract Wastewater, which contains everything from pathogens to pollutants, is a geospatially-and temporally-linked microbial fingerprint of given population. As result, it can be leveraged for monitoring multiple dimensions public health across locales and time. Here, we integrate targeted bulk RNA sequencing (n=1,419 samples) track the viral, bacterial, functional content over geospatially distinct areas within Miami Dade County 2020-2022. First, used amplicon (n=966) diverse SARS-CoV-2...
Abstract Background: Pancreatic cancer is one of the most difficult cancers to detect early and patients die from complications arising due distant organ metastases. The lack bona fide biomarkers primary reasons for late diagnosis this cancer. Most researchers have focused on screening strategies characterize that show promise in preclinical models but eventually fail a clinical scenario. multifactorial disease warrants multi-omics approach identify novel diagnosis. Methods: In order...