A5037752476- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Molecular Biology Techniques and Applications
- Genomics and Phylogenetic Studies
- Genetics, Bioinformatics, and Biomedical Research
- Biomedical and Engineering Education
- Gene expression and cancer classification
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Poxvirus research and outbreaks
- RNA modifications and cancer
- Bacillus and Francisella bacterial research
- Cancer Genomics and Diagnostics
- COVID-19 Clinical Research Studies
- Vibrio bacteria research studies
- Scientific Computing and Data Management
- Cancer-related molecular mechanisms research
- SARS-CoV-2 and COVID-19 Research
- Dental Research and COVID-19
- Gut microbiota and health
- Advanced Biosensing Techniques and Applications
- COVID-19 diagnosis using AI
- Sustainability and Climate Change Governance
- Nanopore and Nanochannel Transport Studies
- Bacterial Genetics and Biotechnology
University of Miami
2020-2024
Sylvester Comprehensive Cancer Center
2020-2024
University of Pennsylvania
2021
Cornell University
2007-2019
Core Laboratories (United States)
2009-2011
Harvard University
2005-2006
Harvard University Press
2006
Mass General Brigham
2006
Brigham and Women's Hospital
2005
International Centre for Diarrhoeal Disease Research
2005
To understand the health impact of long-duration spaceflight, one identical twin astronaut was monitored before, during, and after a 1-year mission onboard International Space Station; his served as genetically matched ground control. Longitudinal assessments identified spaceflight-specific changes, including decreased body mass, telomere elongation, genome instability, carotid artery distension increased intima-media thickness, altered ocular structure, transcriptional metabolic DNA...
Maize is an important crop species of high genetic diversity. We identified and genotyped several million sequence polymorphisms among 27 diverse maize inbred lines discovered that the genome was characterized by highly divergent haplotypes showed 10- to 30-fold variation in recombination rates. Most chromosomes have pericentromeric regions with suppressed appear influenced effectiveness selection during development may be a major component heterosis. found hundreds selective sweeps...
Abstract Background Pseudomonas aeruginosa is a ubiquitous environmental bacterium and an important opportunistic human pathogen. Generally, the acquisition of genes in form pathogenicity islands distinguishes pathogenic isolates from nonpathogens. We therefore sequenced highly virulent strain P. , PA14, compared it with previously (and less pathogenic) strain, PAO1, to identify novel virulence genes. Results The PA14 PAO1 genomes are remarkably similar, although has slightly larger genome...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in wastewater has been used to track community infections of coronavirus disease-2019 (COVID-19), providing critical information for public health interventions. Since levels are dependent upon human inputs, we hypothesize that tracking can be improved by normalizing concentrations against indicators waste [Pepper Mild Mottle Virus (PMMoV), β-2 Microglobulin (B2M), and fecal coliform]. In this study, analyzed SARS-CoV-2 from two...
Non-O1, non-O139 Vibrio cholerae can cause gastroenteritis and extraintestinal infections, but, unlike O1 O139 strains of V. , little is known about the virulence gene content non-O1, their phylogenetic relationship to other pathogenic . Comparative genomic microarray analysis four indicates that these are quite divergent from strains. Genomic sequence a strain (AM-19226) appeared particularly in experimental animals suggests this carries type III secretion system (TTSS) related TTSS2...
To fully understand how bacteria respond to their environment, it is essential assess genome-wide transcriptional activity. New high-throughput sequencing technologies make possible query the transcriptome of an organism in efficient unbiased manner. We applied a strand-specific method sequence bacterial transcripts using Illumina's technology. The resulting sequences were used construct profiles. Novel bioinformatics analyses developed and combination with proteomics data for qualitative...
Methods of wastewater concentration (electronegative filtration (ENF) versus magnetic bead-based (MBC)) were compared for the analysis severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), beta-2 microglobulin, and human coronavirus OC43. Using ENF as method, two quantitative polymerase chain reaction (qPCR) analytical methods also compared: volcano second generation (V2G)-qPCR reverse transcriptase (RT)-qPCR measuring three different targets virus responsible COVID-19 illness (N1,...
Whole‐genome association studies of complex traits in higher eukaryotes require a high density single nucleotide polymorphism (SNP) markers at genome‐wide coverage. To design high‐throughput, multiplexed SNP genotyping assays, researchers must first discover large numbers SNPs by extensively resequencing multiple individuals or lines. For discovery approaches using short read‐lengths that next‐generation DNA sequencing technologies offer, the highly repetitive and duplicated nature plant...
RNA-Seq has provided valuable insights into global gene expression in a wide variety of organisms. Using modified approach and Illumina's high-throughput sequencing technology, we globally identified 5′-ends transcripts for the plant pathogen Pseudomonas syringae pv. tomato str. DC3000. A substantial fraction obtained by this method were consistent with results using 5′RACE. As expected, many positioned short distance upstream annotated genes. We also captured within intergenic regions,...
The MetaSUB Consortium, founded in 2015, is a global consortium with an interdisciplinary team of clinicians, scientists, bioinformaticians, engineers, and designers, members from more than 100 countries across the globe. This network has continually collected samples urban rural sites including subways transit systems, sewage hospitals, other environmental sampling. These collections have been ongoing since 2015 continued when possible, even throughout COVID-19 pandemic. optimized their...
Wastewater-based epidemiology (WBE) has been utilized to track community infections of Coronavirus Disease 2019 (COVID-19) by detecting RNA the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), within samples collected from wastewater. The correlations between and wastewater measurements can potentially change as SARS-CoV-2 evolves into new variations mutating. This study analyzed indicators human waste in two sewersheds different scales (University Miami (UM) campus Miami-Dade...
Wastewater-based surveillance (WBS) is a noninvasive, epidemiological strategy for assessing the spread of COVID-19 in communities. This was based upon wastewater RNA measurements viral target, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The utility WBS has motivated research to measure targets beyond SARS-CoV-2, including pathogens containing DNA. objective this study establish necessary steps isolating DNA from by modifying long-standing RNA-specific extraction workflow...
The COVID-19 pandemic has had a profound, detrimental effect on economies and societies worldwide.Where the been controlled, extremely high rates of diagnostic testing for SARS-CoV-2 virus have proven critical, enabling isolation cases contact tracing.Recently, supplemented with wastewater measures to evaluate degree which communities infections.Whereas much done through traditional, centralized, clinical, or environmental laboratory methods, point-of-care successful in reducing time...