A5000601534- Gut microbiota and health
- Diet and metabolism studies
- Pancreatic and Hepatic Oncology Research
- Tryptophan and brain disorders
- Cancer Cells and Metastasis
- Clostridium difficile and Clostridium perfringens research
- Pediatric Pain Management Techniques
- Pancreatitis Pathology and Treatment
- Extracellular vesicles in disease
- Anaerobic Digestion and Biogas Production
- Neuroinflammation and Neurodegeneration Mechanisms
- Ruminant Nutrition and Digestive Physiology
- Microbial Metabolic Engineering and Bioproduction
- 3D Surveying and Cultural Heritage
- Robotics and Sensor-Based Localization
- Stress Responses and Cortisol
- IoT Networks and Protocols
- Single-cell and spatial transcriptomics
- Advanced MIMO Systems Optimization
- Satellite Communication Systems
- Genomics and Phylogenetic Studies
- HIV/AIDS oral health manifestations
- Prenatal Substance Exposure Effects
- Gastrointestinal motility and disorders
- Cancer, Stress, Anesthesia, and Immune Response
University of Miami
2020-2025
Ningxia Medical University
2025
Sylvester Comprehensive Cancer Center
2021-2024
Stony Brook University
2024
State Key Laboratory of Industrial Control Technology
2022-2023
Yunnan University
2022
Beijing Institute of Petrochemical Technology
2020
University of Florida
2016-2019
Huazhong University of Science and Technology
2017
Fudan University
2008
Opioid crisis is an ongoing epidemic since the past several decades in United States. use-associated microbial dysbiosis emerging as a key regulator of intestinal homeostasis and behavioral responses to opioid. However, mechanistic insight into role community modulating host response unavailable. To uncover opioid-induced disrupting we utilized whole genome sequencing, untargeted metabolomics, mRNA sequencing identify changes microbiome, metabolome, transcriptome respectively. Morphine...
Summary Bacteria have been thought to follow only a few well‐recognized biochemical pathways when fermenting glucose or other hexoses. These chiseled in the stone of textbooks for decades, with most sources rendering them as they appear classic 1986 text by Gottschalk. Still, it is unclear how broadly these apply, given that were established and delineated biochemically model organisms. Here, we show often cannot explain fermentation products formed bacteria. In extensive analysis its kind,...
There has been a rapid increase in neonates born with history of prenatal opioid exposure. How exposure affects pain sensitivity offspring is interest, as this may perpetuate the epidemic. While few studies have reported hypersensitivity to thermal pain, potential mechanisms not described. This study posits that alterations gut microbiome underly prenatally methadone-exposed 3-week-old male offspring, which were generated using mouse model methadone Fecal samples collected from dams and...
This study aims to evaluate the clinical efficacy of pancreatic duct stenting in treatment SAP, providing reference for diagnosis and treatment. A retrospective analysis was conducted on data from patients with SAP admitted General Hospital Ningxia Medical University June 1, 2019 December 31, 2022. total 51 were included (33 males, 18 females). Patients divided into two groups based treatment: control group (n = 28) receiving conventional stent 23) undergoing addition Data collected analyzed...
Abstract The development of anxiety during protracted opioid withdrawal heightens the risk relapse into cycle addiction. Understanding mechanisms driving could facilitate therapeutics to prevent negative affect and promote continued abstinence. Our lab has previously established gut microbiome as a driver various side effects use, including analgesic tolerance somatic symptoms. We therefore hypothesized that contributes anxiety-like behavior withdrawal. In this study, we first mouse model...
Opioids, such as morphine, are used in the Neonatal Intensive Care Unit (NICU) for pain relief neonates. However, available evidence concerning benefits and harms of opioid therapy neonates remains limited. While previous studies have reported that neonatal morphine exposure (NME) results long-term heightened sensitivity, underlying mechanisms not well understood. This study proposes dysbiosis gut microbiome contributes to hypersensitivity following NME. Using an adolescent female murine...
The gut microbiome has emerged as a promising target for modulating adverse effects of opioid exposure due to its significant role in health and disease. Opioid use disorder (OUD) become increasingly prevalent, specifically women reproductive age, contributing an increased incidence offspring exposed opioids utero. Recent studies have shown that prenatal (POE) is associated with notable changes the maternal microbiome, subsequent implications offspring's other outcomes. However, mediating...
Fluorescent tracers have been used to measure solute transport, but transport kinetics not evaluated by comparison of radiolabeled tracers. Using Streptococcus equinus JB1 and other bacteria, the objective this study was determine if a fluorescent analogue glucose (2-NBDG) would be transported with same transporters as [14C]glucose. We uniquely modified technique for measuring so that tracer could also measured. Deploying S. JB1, we detect 2-NDBG quantitatively within 2 s. found Vmax 2-NBDG...
The functional relevance of intra-species diversity in natural microbial communities remains largely unexplored. guts two closely related honey bee species, Apis cerana and A. mellifera, are colonised by a similar set core bacterial species composed host-specific strains, thereby providing good model for an study. We aim to assess the mellifera gut microbiota. Honey workers were collected from four regions China. Their microbiomes investigated shotgun metagenomic sequencing, compositions...
The ongoing opioid epidemic has left millions of people suffering from use disorder due to the over-prescription highly addictive substances. Chronic exposure leads dependence, where absence drug results in negative symptoms withdrawal, often driving patients continue use; however, few therapeutic strategies are currently available combat cycle addiction and severity morphine withdrawal. This study investigates microbiome as a potential target for gut dysbiosis caused by been proven...
Abstract Background Pancreatic cancer is one of the most difficult cancers to detect early and patients die from complications arising due distant organ metastases. The lack bona fide biomarkers primary reasons for late diagnosis pancreatic cancer. It a multifactorial disease warrants novel approach identify biomarkers. Methods In order characterize proteome, Extracellular vesicles (EVs) isolated different in vitro conditions mimicking tumor-microenvironment interactions between epithelial...
Opioids, such as morphine and oxycodone, are widely used for pain management associated with chronic pancreatitis (CP); however, their impact on the progression sensitivity of CP has never been evaluated. This report investigates opioid use severity CP, sensitivity, gut microbiome. C57BL/6 mice were divided into control, morphine/oxycodone, either or oxycodone alone groups. was induced by administration caerulein (50ug/kg/h, i.p. hourly x7, twice a week 10 weeks). The mouse-to-pancreas...
This study characterized compositional and functional shifts in the intestinal oral microbiome HIV-positive patients on antiretroviral therapy compared to HIV-negative individuals. Seventy-nine specimens were collected from 5 12 control subjects five locations (colon brush, colon wash, terminal ileum [TI] TI saliva) during colonoscopy at patient visits. Microbiome composition was using 16S rRNA sequencing, function predicted bioinformatics tools (PICRUSt BugBase). Our analysis indicated that...
Few characteristics are more important to a bacterium than the substrates it consumes. It is hard identify what consumed by bacteria in natural communities, however, because most have not been cultured. In this study, we developed method that uses fluorescent substrate analogs, cell sorting, and DNA sequencing taken up bacteria. We deployed using 2[N-(7-nitrobenz-2-oxa-1,2-diaxol-4-yl)amino]-2-deoxyglucose (2-NBDG), glucose analog, of bovine rumen. This revealed over 40 different (amplicon...
Despite the many advancements in field of pain management, use intravenous opioids, such as morphine, neonates is still a challenge for clinicians and researchers, available evidence concerning long-term consequences an early exposure limited. In particular, little known neonatal morphine on gut microbiome, which has been identified key modulator health diseases. Consequently, purpose this study was to investigate those microbiome. Newborn mice were exposed either (5 mg/kg/day) or saline...
The gut microbial ecosystem exhibits a complex bidirectional communication with the host and is one of key contributing factors in determining mucosal immune homeostasis or an inflammatory state. Opioid use has been established to induce dysbiosis consistent increased intestinal tissue inflammation. In this study, we investigated role infiltrated cells morphine-induced damage mice. Results reveal significant increase chemokine expression tissues followed by neutrophil infiltration post...
Background and Purpose Opioids are commonly used for the management of cancer-associated pain chemotherapy-induced diarrhoea. The chemotherapeutic irinotecan (CPT-11) causes severe gastrointestinal (GI) toxicity due to deconjugation inactive metabolite SN-38 glucuronide (SN-38G) by bacterial β-glucuronidases active 7-ethyl-10-hydroxycamptothecin (SN-38). known cause gut microbial dysbiosis, this study evaluated whether CPT-11 anti-tumour efficacy GI exacerbated opioid co-administration....
The cycle of substance use disorder (SUD) leading to dependence is a complex process involving multiple neurocircuitries and brain regions. amygdala the core region that involved in processing withdrawal anxiety depressive-like behaviors. However, transcriptional changes each cell type within during SUD remains unknown. Here, we performed single-cell RNA sequencing classified all types mouse amygdala. We particularly focused on gene expression glial cells under dependent state compared...
When given excess carbohydrate, certain microbial species respond by storing energy (synthesizing reserve carbohydrate), but other dissipating the as heat (spilling energy). To determine importance of these responses in rumen community, this study quantified mixed ciliate protozoa vs bacteria to glucose. We hypothesized that ciliates would direct more glucose synthesis carbohydrate (and less spilling) than bacteria. Ciliates and were isolated from fluid using filtration centrifugation,...
The accurate and reliable high-altitude orientation estimation is of great significance for unmanned aerial vehicles (UAVs) localization, further assists them to conduct some fundamental functions, such as mapping, environmental monitoring, risk management. However, the traditional susceptible electromagnetic interference, high maneuverability, substantial scale variations. Hence, this paper aims present a new visual compass algorithm estimate UAV employing appearance geometry structure...