A5108003000- Spaceflight effects on biology
- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Space Exploration and Technology
- Medical and Biological Ozone Research
- Renal cell carcinoma treatment
- European Political History Analysis
- Mesenchymal stem cell research
- Anesthesia and Neurotoxicity Research
- High Altitude and Hypoxia
- Mitochondrial Function and Pathology
- Engineering and Materials Science Studies
- Science, Research, and Medicine
- Cancer Treatment and Pharmacology
- Breast Cancer Treatment Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Immunotherapy and Biomarkers
- Environmental Toxicology and Ecotoxicology
- 3D Printing in Biomedical Research
- Cell death mechanisms and regulation
- Osteoarthritis Treatment and Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Cardiovascular and Diving-Related Complications
- Heat shock proteins research
- Neonatal Respiratory Health Research
Centre d'études de l'emploi
2016
University of Lausanne
2016
University of Vienna
2015
Max Planck Institute for Legal History and Legal Theory
2015
Poliklinik für Zahnärztliche Prothetik
2015
University of Hohenheim
2011-2014
Charité - Universitätsmedizin Berlin
1986-2011
Freie Universität Berlin
2010
Humboldt-Universität zu Berlin
1986
Extracellular matrix proteins, adhesion molecules, and cytoskeletal proteins form a dynamic network interacting with signalling molecules as an adaptive response to altered gravity. An important issue is the exact differentiation between real microgravity responses of cells or cellular reactions hypergravity and/or vibrations. To determine effects on human cells, we used four DLR parabolic flight campaigns focused short-term (22 s), (1.8 g), vibrations ML-1 thyroid cancer cells. No signs...
This study focused on the effects of short-term microgravity (22 s) gene expression and morphology endothelial cells (ECs) evaluated gravisensitive signaling elements. ECs were investigated during four German Space Agency (Deutsches Zentrum für Luft- und Raumfahrt) parabolic flight campaigns. Hoechst 33342 acridine orange/ethidium bromide staining showed no signs cell death in after 31 parabolas (P31). Gene array analysis revealed 320 significantly regulated genes first parabola (P1) P31....
Abstract Many space missions have shown that prolonged flights may increase the risk of cardiovascular problems. Using a three‐dimensional clinostat, we investigated human endothelial EA.hy926 cells up to 10 days under conditions simulated microgravity (µg) distinguish transient from long‐term effects µg and 1g. Maximum expression all selected genes occurred after min clinorotation. Gene (osteopontin, Fas, TGF‐β 1 ) declined slightly upregulated levels or rose again (caspase‐3) fourth day...
Endothelial cells (ECs) form three-dimensional (3D) aggregates without any scaffold when they are exposed to microgravity simulated by a random positioning machine (RPM) but not under static conditions at gravity. Here we describe delayed type of formation 3D structures ECs that was initiated cultured on desktop RPM remained adherent for the first 5 days spread over neighboring cells, forming little colonies. After 2 weeks, tube-like (TSs) became visible in these cultures. They included...
When incubated under simulated microgravity (s-microg), endothelial cells (EC) form tubular structures that resemble vascular intimas. This delayed formation of 3D EC begins between the 5th and 7th day culturing conditions s-microg, when double-row cell assemblies become visible. With aim learning about this initial phase structure formation, we found NFkappaBp65 protein content was similar in all populations, but gene expression phosphokinase A catalytic subunit, Calpha, extracellular...
Tissue engineering is a strategy of cartilage regeneration, but scaffolds, required for 3D growth chondrocytes, are still problem.Searching possibilities to improve scaffold-free cartilage, we characterized human chondrocytes incubated on random positioning machine (RPM) simulate microgravity (microg).When cultured in simulated microg, start forming cell assemblies within 5 days. After 24h, could not detect caspase-3, Fas, p53 or Bcl-2 proteins these cells, Annexin V flow cytometry, however,...
Abstract Fibroblast growth factors interact with appropriate endothelial cell (EC) surface receptors and initiate intracellular signal cascades, which participate in modulating blood vessel growth. EC, upon exposure to basic fibroblast (bFGFs) undergo profound functional alterations, depend on their actual sensitivity involve gene expression de novo protein synthesis. We investigated the effects of bFGF signaling pathways EA.hy926 cells different environments. EC were cultured under normal...
Chlorophyllin kills mosquito larvae (Culex, Aedes) in the aquatic habitat at low concentrations via photodynamic reactions under irradiation. The effects of chlorophyllin were investigated cellular level using transparent Chaoborus crystallinus as a model system. Their transparency enabled situ fluorescence investigation, showing that accumulates intestine larvae. Uptake room temperature took about 2 h. signal peaked after 5 h incubation. up to 15 ng per treated was dissected and stained...
Background: Myocardium is very sensitive to gravitational changes. During a spaceflight cardiovascular atrophy paired with rhythm problems and orthostatic intolerance can occur. The aim of this study was investigate the impact basic fibroblast growth factor (bFGF) vascular endothelial (VEGF) on cardiac fibroblasts (CF) grown under altered gravity conditions. Methods: We examined influence exposure Random Positioning Machine (RPM) CF, derived from porcine hearts. focused growth, extracellular...
Cet article est la version retravaillée d'une introduction des auteur-e-s au volume Selbstzeugnis und Person. Transkulturelle Perspektiven, synthèse travaux du groupe de recherche « Selbstzeugnisse in transkultureller Perspektive » soutenu entre 2004 et 2012 par Deutsche Forschungsgemeinschaft (DFG). De nouveaux concepts sur les écrits personnels propres à visibiliser multiplicité diversité l'écriture soi dans différentes cultures y sont discutés. Dans un premier temps, interprètent...
Humane Zellen wurden für unterschiedlich lange Zeitspannen, von 22 Sekunden im Parabelflug bis hin zu 2 Wochen auf Satelliten, realer Schwerelosigkeit und Vergleich hierzu einer Random Positioning Machine einem 2D-Klinostaten simulierter ausgesetzt anschließend analysiert. Dabei Erkenntnisse gewonnen, welche nicht nur bei der Erklärung Gesundheitsproblemen Astronauten helfen können, sondern auch die Grundlagenforschung wichtig sind. Weiterhin zeigte sich, dass sich Knorpel-, Schilddrüsen-...