Abstract Warburg effect is an emerging hallmark of cancer cells with pyruvate kinase M2 (PKM2) as its key regulator. Curcumin extensively-studied anti-cancer compound, however, role in affecting metabolism remains poorly understood. Herein, we show that curcumin inhibits glucose uptake and lactate production (Warburg effect) a variety cell lines by down-regulating PKM2 expression, via inhibition mTOR-HIF1α axis. Stable silencing revealed required for proliferation cells. over-expression...

Abstract Background Insulin is tightly associated with cancer progression; however, mechanistic insights into such observations are poorly understood. Recent studies show that metabolic transformation critical to cell proliferation. Here, we attempt understand the role of insulin in promotion metabolism. To this end, regulating glycolytic enzyme pyruvate kinase M2 (PKM2) was examined. Results We observed up-regulated PKM2 expression, through PI3K/mTOR mediated HIF1α induction, but...

Metabolism of cancer cells with pyruvate kinase M2 (PKM2) at its centre stage has assumed a prime significance in research recent times. Cancer cell metabolism, characterized by enhanced glucose uptake, production lactate and anabolism is considered an ideal target for therapeutic interventions. Expression PKM2 switches metabolism favor cells, therefore, the present study was designed to investigate hitherto unknown effect resveratrol, phytoalexin, on expression resultant implications...

Preferential expression of the low-activity (dimeric) M2 isoform pyruvate kinase (PK) over its constitutively active splice variant M1 is considered critical for aerobic glycolysis in cancer cells. However, our results reported here indicate co-expression PKM1 and PKM2 their possible physical interaction We show that knockdown either or differentially affects net PK activity, viability, cellular ATP levels lung carcinoma cell lines H1299 A549. The stable isoforms A549 cells significantly...

Triple-negative breast cancer (TNBC) is an aggressive form of with limited treatment modalities and poor prognosis. Metabolic reprogramming in considered a hallmark therapeutic relevance. Here, we report disruption metabolic TNBC cells by silibinin via modulation EGFR-MYC-TXNIP signaling. assays combined LC-MS-based metabolomics revealed inhibition glycolysis other key biosynthetic pathways silibinin, to induce catastrophe cells. Silibinin-induced suppression resulted decreased cell biomass,...

Cancer cells ferment glucose, even under aerobic conditions, following a phenomenon known as the ‘Warburg effect.’ Hexokinase 2 (HK2) catalyzes crucial step of phosphorylation glucose for subsequent utilization in glycolysis and other pathways. HK2 has been proposed potential therapeutic target anti-cancer therapy because its enhanced expression glucose-dependent tumors. Here, we have employed structure-based virtual screening using in-house library to identify phytoconstituents which could...

Striking similarity exists between metabolic changes associated with embryogenesis and tumorigenesis. Chromobox proteins-CBX2/4/6/7/8, core components of canonical polycomb repressor complex 1, play essential roles in embryonic development aberrantly expressed breast cancer. Understanding how altered CBX expression relates to reprogramming cancer may reveal vulnerabilities therapeutic pertinence. Using transcriptomic metabolomic data from patients (N > 3000 combined), we performed...

The rapid rate of urban expansion due to a rise in population and economic growth is causing land use/land cover changes fringe areas Srinagar city especially the Dudhganga watershed. This paper analyzes that occurred points out major impact urbanization on landscape through multi-temporal Landsat satellite data (1991 2010) In order assess pattern urbanization, consumption ratio (LCR) absorption coefficient (LAC) have been estimated. results revealed throughout period 1991–2010, amount built...

The metabolism of cancer is remarkably different from that normal cells and confers a variety benefits, including the promotion other hallmarks. As rewired near-universal property cells, efforts are underway to exploit metabolic vulnerabilities for therapeutic benefits. In continued search safer effective ways treatment, structurally diverse plant-based compounds have gained substantial attention. Here, we present an extensive assessment role phytocompounds in modulating attempt make case...

The present study was designed to examine the functional relevance of two heterozygous mutations (H391Y and K422R), observed earlier by us in Bloom syndrome condition. Cells stably expressing exogenous wild-type or mutant PKM2 (K422R H391Y) co-expressing both wild type (PKM2-K422R PKM2-H391Y) were assessed for cancer metabolism tumorigenic potential. Interestingly, cells showed significantly aggressive as compared with either independently. A similar trend oxidative endurance, potential,...

Runt related transcription factor3 (RUNX3) is considered as a tumor suppressor gene (TSG) that functions through the TGF-β dependent apoptosis. Promoter methylation of CpG islands RUNX3 and overexpression enhancer zeste homolog 2 (EZH2) has been suggested to downregulate in cancer.Here, we studied expression EZH2 58 esophageal tumors along with paired adjacent normal tissue. mRNA levels, protein expressions cellular localizations were analyzed using real-time PCR immunohistochemistry,...

Nutrient utilization is dramatically altered when cells receive signals to proliferate. Characteristic metabolic changes enable meet the large biosynthetic demands associated with cell growth and division. Changes in rate-limiting glycolytic enzymes redirect metabolism support proliferation. Metabolic reprogramming cancer controlled largely by oncogenic activation of signal transduction pathways transcription factors. Although less well understood, epigenetic mechanisms may seem contribute...

Metabolic changes that contribute to differentiation are not well understood. Overwhelming evidence shows the critical role of glycolytic enzyme pyruvate kinase (PK) in directing metabolism proliferating cells. However, its differentiating cells is unclear. Here we studied PK phorbol 12-myristate 13-acetate (PMA)-induced megakaryocytic human leukemia K562 We observed PMA treatment decreased cancer-type anabolic but increased ATP production, along with up-regulated expression two isoforms...

O-6-methylguanine-DNA methyltransferase, DNA repair gene, has been found to be involved with the pathogenesis of esophageal cancer. hypermethylation and other factors have suggested downregulate methyltransferase. In this communication, methylation status methyltransferase gene corresponding protein expression in cancer from North India studied. all, 80 samples tumor tissue along adjacent normal as controls were analyzed for messenger RNA level expression, subcellular localization. The was...

Volleyball involves repeated forceful arm actions that produce a high incidence of shoulder injury. Shoulder rotator strength ratio is an important predictor the likelihood injury, especially, secondary impingement and instability. Therefore, assessment muscle external internal rotators imperative to establish profiles performance, ratios, mobility volleyball players.To reliability validity EN-TreeM dynamometer for measurement in players.Thirty male players aged 18-24 years, mean height...

Abstract A new inorganic ion exchanger zinc silicate has been synthesized. Its properties such as exchange capacity, heat effect and stability etc. have studied. Sorption of PAN over formed a chelate which showed greater selectivity for some metal ions especially Cu2+, Ni2+, Co2+, Fe3+, Ag+, Ag3+ Pt4+. Selectivity determined on the basis distribution coefficients these ions. Separations Pt(IV) from Fe(III), Au(III) Ag(I) Cu(II) reported. The recovery dilute solutions also