A5006096312- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- NF-κB Signaling Pathways
- Immune Cell Function and Interaction
- Histone Deacetylase Inhibitors Research
- Xenotransplantation and immune response
- Viral Infections and Immunology Research
- melanin and skin pigmentation
- Virus-based gene therapy research
South China Agricultural University
2021-2025
HDAC6 modulates viral infection through diverse mechanisms. Here, we investigated the role of in influencing pig cells with aim exploiting potential antiviral gene targets pigs. Using knockout and overexpression strategies, found that greatly reduced PRV VSV infectivity, whereas increased their infectivity PK15 cells. Mechanistic studies identified as a DNA damage inhibitor attenuated levels, which can further reduce type I IFN production to promote infection. Conversely, deficiency limit by...
Abstract Background As an important farm animal, pig functional genomic study can help understand the molecular mechanism related to key economic traits of pig, such as growth, reproduction, or disease. The genome‐scale library based on clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated endonuclease Cas9 (Cas9) system facilitates discovery genes involved in a specific function phenotype, allowing for effective “phenotype‐to‐genotype” strategy study. Methods...
Black coat color in pigs is determined by the dominant E allele at MC1R locus. Through comparing gene sequences between recessive e and ED1 alleles, we identified four missense mutations that could affect protein function for eumelanin synthesis. With aim of devising a genetic modification method pig manipulation, mutated Duroc breed to using CRISPR-mediated homologous recombination mutation substitutions The MC1R-modified generated replacement strategy displayed uniform black across body. A...
Gene-modified miniature pigs serve as alternative tissue and organ donors for xenotransplantation to alleviate the shortage of human allogenic organs. However, high copy number porcine endogenous retrovirus (PERV) genomes integrates with genome, which has a potential risk cross-species transmission hinders clinical practice xenotransplantation. Recently, CRISPR/Cas9 been used inactivate PERVs. Cas9 also triggers severe DNA damage at multiple integrated PERV sites in induces senescence...