A5022406930- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- Macrophage Migration Inhibitory Factor
- Microtubule and mitosis dynamics
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Erythrocyte Function and Pathophysiology
- Hemoglobin structure and function
- Amyotrophic Lateral Sclerosis Research
- Blood properties and coagulation
- Neurogenetic and Muscular Disorders Research
- Ubiquitin and proteasome pathways
- Transgenic Plants and Applications
Scripps Research Institute
2024
Scripps (United States)
2024
Scripps Institution of Oceanography
2024
University of California, San Francisco
2016-2022
QB3
2022
Innovative Genomics Institute
2016
University of California, Berkeley
2016
We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA (Horlbeck et al., 2016). Here, we build on this observation with a comprehensive algorithm incorporates chromatin, position, and sequence features accurately predict highly effective single guide RNAs (sgRNAs) for targeting nuclease-dead Cas9-mediated transcriptional repression (CRISPRi) activation (CRISPRa). use design next-generation genome-scale CRISPRi CRISPRa libraries human mouse genomes. A screen essential...
CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions cell biology, genetics, biotechnology, but wider deployment CRISPRi screening has been constrained by large size single guide RNA (sgRNA) libraries challenges generating models with consistent knockdown. Here, we present next-generation sgRNA effector...
Cop9 signalosome (CSN) regulates the function of cullin-RING E3 ubiquitin ligases (CRLs) by deconjugating ubiquitin-like protein NEDD8 from cullin subunit. To understand physiological impact CSN on CRL network and cell proliferation, we combined quantitative mass spectrometry genome-wide CRISPR interference (CRISPRi) activation (CRISPRa) screens to identify factors that modulate viability upon inhibition small molecule CSN5i-3. components regulators strongly modulated antiproliferative...
The propeller-shaped blades of the PIEZO1 and PIEZO2 ion channels partition into plasma membrane respond to indentation or stretching lipid bilayer, thus converting mechanical forces signals that can be interpreted by cells, in form calcium flux changes potential. While PIEZO participate diverse physiological processes, from sensing shear stress blood flow vasculature detecting touch through mechanoreceptors skin, molecular details enable these mechanosensors tune their responses over a vast...
Abstract CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions cell biology, genetics, biotechnology, but wider deployment CRISPRi screening has been constrained by large size single guide RNA (sgRNA) libraries challenges generating models with consistent knockdown. Here, we present next-generation sgRNA...