A5026806378- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Air Traffic Management and Optimization
- Advanced Materials and Mechanics
- Health Systems, Economic Evaluations, Quality of Life
- Per- and polyfluoroalkyl substances research
- Adhesion, Friction, and Surface Interactions
- UAV Applications and Optimization
- Computational Drug Discovery Methods
- Aerospace and Aviation Technology
- Spacecraft Design and Technology
- Structural Analysis and Optimization
- Gene expression and cancer classification
- Autonomous Vehicle Technology and Safety
- Robotic Path Planning Algorithms
- Transgenic Plants and Applications
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Veterinary Equine Medical Research
- Genetics, Bioinformatics, and Biomedical Research
- Machine Learning in Healthcare
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Toxic Organic Pollutants Impact
- Nanofabrication and Lithography Techniques
VIR Biotechnology (United States)
2024
ID Genomics (United States)
2021-2022
Broad Institute
2015-2020
Harvard–MIT Division of Health Sciences and Technology
2015-2019
Princeton University
2013-2016
Massachusetts Institute of Technology
2016
Both intrinsic cell state changes and variations in the composition of stem populations have been implicated as contributors to aging. We used single-cell RNA-seq dissect variability hematopoietic (HSC) progenitor from young old mice two strains. found that cycle dominates within each population there is a lower frequency cells G1 phase among compared with long-term HSCs, suggesting they traverse through faster. Moreover, transcriptional HSCs during aging are inversely related those upon HSC...
Tissues contain exquisite vascular microstructures, and patterns of chemical cues for directing cell migration, homing, differentiation organ development function. 3D microfabrication by multi‐photon photolithography is a flexible, high‐resolution tool generating bioscaffolds. However, the combined fabrication scaffold microstructure simultaneously with patterning to create both geometrically chemically defined microenvironments remains be demonstrated. This study presents high‐speed method...
In experimental evolution, scientists evolve organisms in the lab, typically by challenging them to new environmental conditions. How best a desired trait? Should challenge be applied abruptly, gradually, periodically, sporadically? one apply chemical mutagenesis, and do strains with high innate mutation rate faster? What are ideal population sizes of evolving populations? There endless strategies, beyond those that can exposed individual labs. We therefore arranged community challenge,...
Early developmental specification can be modeled by differentiating embryonic stem cells (ESCs) to embryoid bodies (EBs), a heterogeneous mixture of three germ layers. Here, we combine single-cell transcriptomics and genetic recording characterize EB differentiation. We map transcriptional states along time course model cell fate trajectories branchpoints as progress distinct To validate this inferential model, propose an innovative inducible technique that leverages recombination generate...
Abstract As part of the process preparing sequencing libraries that include unique molecular identifiers (UMIs) such as many single cell RNA-seq (scRNA-seq) libraries, a diverse template must be amplified. During amplification, spurious chimeric molecules can formed between originating in different cells. While several computational and experimental strategies have been suggested to mitigate impact molecules, suitable approaches for scRNA-seq experiments do not exist. We demonstrate chimeras...
While the clinical approval process is able to filter out medications whose utility does not offset their adverse drug reaction profile in humans, it well suited characterizing lower frequency issues and idiosyncratic multi-drug interactions that can happen real world diverse patient populations. With a growing abundance of real-world evidence databases containing hundreds thousands records, now feasible build machine learning models incorporate individual information provide personalized...
The gradual in-plane compression of a solid film bonded to soft substrate can lead surface wrinkling and even the formation network folds for sufficiently high strain. An understanding how these initiate, propagate, interact with each other is still lacking. In previous study, we developed an experimental system observe wrinkle-to-fold transition layered elastic materials under biaxial compressive stresses. Here focus on dynamic interaction pair propagating compression. We find...
Inexpensive components that can be used to create an unmanned air vehicle (UAV) are becoming increasingly available. This accessibility opens up exciting applications: from automated resource transportation in developing countries the scale testing of integrated airspace systems. We present a novel UAV system which guidance, navigation, and control were performed on onboard iPhone 4 desired trajectory was supplied through ground based laptop. Additional UAVs modularly cooperatively interact...
Novel airspace systems that integrate the latest technology advancements must be tested before widespread adoption. Despite growing sophistication of simulation software, new practically implementation. To facilitate rapid prototyping such architectures we present flight test results a low cost, small scale, modular design. We incorporate data processing and control logic into an onboard iPhone 4 in UAV aircraft. The performs guidance, navigation, control, while receiving desired trajectory...
Advances in technology have partially ameliorated the increasing workload on air traffic controllers due to tremendous volume of traffic. However, higher level automation will be necessary, especially considering pressures current airspace architecture by unmanned aerial vehicles (UAVs). Here we present a relatively inexpensive, modular, and wireless networked airspace. We system as test bed for implementation distributed system, any associated ground based or airborne algorithms. As proof...
Regulatory agencies are beginning to recognize and regulate per-and polyfluoroalkyl substances (PFAS) as concerning environmental contaminants. In groundwater management, testing mitigation strategies desirable, but can be time cost-intensive processes. As a result, only fraction of all wells has been tested for PFAS levels, resulting in potentially extended drinking water exposure the meantime. this study, we build series machine learning models (including linear random forest regressors)...
Abstract Genetic interactions, defined as the non-additive phenotypic impact of combinations genes, are a hallmark mapping from genotype to phenotype. However, genetic interactions remain challenging systematically test given massive number possible combinations. In particular, while large-scale screening efforts in yeast have quantified pairwise that affect cell viability, or synthetic lethality, between all pairs genes well for limited three-way it has previously been intractable perform...
Abstract Recent growth in population scale sequencing initiatives involve both cohort and proportion of genome surveyed, with a transition from genotyping arrays to broader approaches. The resulting datasets can be challenging analyze. Here we introduce DNARecords novel sparse-compatible format for large genetic data. structure enables integration complex data types such as medical images drug structures towards the development machine learning methods predict disease risk response. We...